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  • 1
    Electronic Resource
    Electronic Resource
    Distribution of the 1,25 dihydroxy-vitamin D3 receptor in the bursa of Fabricius of chicken (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 838-841 
    Details
    ISSN: 1420-9071
    Keywords: Vitamin D3 receptor ; dihydroxy-vitamin D3 ; bursa of Fabricius ; ontogeny of immune system ; B lymphocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The vitamin D3 metabolite 1,25(OH)2D3 is probably involved in B lymphocyte ontogeny. We therefore determined the distribution of the 1,25(OH)2D3 receptor in the bursa of Fabricius and spleen cells of 7-day-old chicks, by immunohistochemistry using a monoclonal antibody against the chick intestinal cell 1,25(OH)2D3 receptor. The bursa cells of young (7-day-old) chicks contained large amounts of receptor while the spleen cells did not. The bursa cells of older (35-day-old) chicks contained fewer receptors, but the number of receptors in the spleen increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01922468
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of multidrug resistance (mdr) gene(s) in primary lymphoid organs of chicken immune system during embryonic development (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 137-140 
    Details
    ISSN: 1420-9071
    Keywords: Chicken ; thymus ; bursa of Fabricius ; MDR ; mdr genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The presence of a multidrug resistance (MDR) related protein, P-170, in normal and pathological lymphoid cells has been described. The present report evaluates the expression of themdr 1 gene by using the reverse Polymerase Chain Reaction (PCR) on cells obtained from the thymus and bursa of chicken embryos starting from day 12 until hatching. Results show that the thymic cells are positive from day 12 to the end of the observation period. In contrast,mdr mRNA was detected in the bursa from day 14 to day 17 of embryonic life. Possible relationships between the expression ofmdr and the development of T and B lymphocytes are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01929357
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro morphological characterization of the bursal reticuloepithelial (REp) cells of chicken (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 1060-1063 
    Details
    ISSN: 1420-9071
    Keywords: Bursa of Fabricius ; cell culture ; cytokeratin ; REp cells ; electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The REp cells of the bursa follicle medulla of chicken were isolated in vitro. Culture of the REp cells was maintained over a period of 10 days and the cells were observed at 3 and 10 days by means of transmission electron microscopy (TEM) and immunofluorescence. The use of an anticytokeratin monoclonal antibody confirmed their epithelial nature. TEM observations showed the presence of desmonsomes and tonofilaments, which are characteristic of epithelial cells. Furthermore, to some extent the cells regenerated in vitro the network they form in vivo. Though the growth rate becomes slower with time, the features of the REp cells do not significantly change.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940673
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  • 4
    Electronic Resource
    Electronic Resource
    An immunohistochemical study of immunological phenomena in minor salivary glands in patients with Sjögren's syndrome (1995)
    Springer
    Rheumatology international 15 (1995), S. 51-55 
    Details
    ISSN: 1437-160X
    Keywords: Sjögren's syndrome ; HLA molecules ; Adhesion molecules ; TCR γδ cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Using different monoclonal antibodies, we performed an immunofluorescent technique on labial salivary glands in order to investigate the immunological phenomena involved in Sjögren's syndrome (SS). An aberrant expression of HLA-DR molecules was detected on cytoplasm of epithelial labial salivary cells in 9 out of 19 (47%) patients, with SS. No such expression was found in 8 patients without SS or in 3 normal controls. HLA-DQ molecules were demonstrated also in two out of ten SS patients without HLA-DR. A lymphocytic infiltration was not correlated with the expression of class II molecules. T cells bearing γδ receptors were not detected. The intracellular adhesion molecules (ICAM-1) and lymphocyte function associated antigen-1 (LFA-1) were not found on epithelial glandular salivary cells of patients and controls. In conclusion, these data suggested that the absence of ICAM-1 and LFA-1 in salivary cells and the absence of infiltrating T cells bearing γδ receptors exclude their immunopathogenetic role in SS; moreover, these data demonstrated that the aberrant expression of HLA class II molecules on epithelial salivary cells of patients with SS is not a phenomenon correlated with the lymphocytic infiltration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262708
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  • 5
    Electronic Resource
    Electronic Resource
    Mutagenic activity and chemical analysis of airborne particulates collected in Pisa (Italy) (1994)
    Springer
    Bulletin of environmental contamination and toxicology 52 (1994), S. 465-473 
    Details
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197838
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  • 6
    Electronic Resource
    Electronic Resource
    Phytoplankton distribution in relation to sea ice, hydrography and nutrients in the northwestern Weddell Sea in early spring 1988 during EPOS (1992)
    Springer
    Polar biology 12 (1992), S. 225-235 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Phytoplankton biomass and distribution of major phytoplankton groups were investigated in relation to sea ice conditions, hydrography and nutrients along three north-south transects in the north western Weddell Sea in early spring 1988 during the EPOS Study (European Polarstern Study), Leg 1. Three different zones along the transects could be distinguished: 1) the Open Water Zone (OWZ) from 58° to 60°S with high chlorophyll a concentrations up to 3.5 μg l−1; 2) the Marginal Ice Zone (MIZ) from 60° to about 62.5° with chlorophyll a concentrations between 0.1 and 0.3 μg l−1, and 3) the closed pack-ice zone (CPI) from 62.5° to 63.2°S with chlorophyll a concentrations below 0.1 μgl−1. Nutrient concentrations increased towards the south showing winter values under the closed pack-ice. Centric diatoms such as Thalassiosira gravida and Chaetoceros neglectum forming large colonies dominated the phytoplankton assemblage in terms of biomass in open water together with large, long chain forming, pennate diatoms, whereas small pennate diatoms such as Nitzschia spp., and nanoflagellates prevailed in ice covered areas. Fairly low concentrations of phytoplankton cells were encountered at the southernmost stations and many empty diatom frustules were found in the samples. The enhanced phytoplankton biomass in the Weddell-Scotia-Confluence area is achieved through sea ice melting in the frontal zone of two different water masses, the Weddell and the Scotia Sea surface waters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238264
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  • 7
    Electronic Resource
    Electronic Resource
    Multilobated B cell lymphoma (Pinkus variant) with isolated muscular localization in a patient with systemic sclerosis: a case report (1996)
    Springer
    Annals of hematology 73 (1996), S. 43-45 
    Details
    ISSN: 1432-0584
    Keywords: Key words Systemic sclerosis ; non-Hodgkin's lymphoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The association of systemic sclerosis (SSc) and non-Hodgkin's lymphoma (NHL) is a rare event whose pathogenesis remains to be clarified. We describe a case of NHL in a patient with SSc, in whom the NHL was particular in terms of clinical presentation (primitive muscle and bone involvement) and histologic type (large multilobated B-cell variant). The lymphoma was clinically aggressive but responded well to therapy. Surveillance for the development of "atypical" NHL is suggested in SSc cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002770050200
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  • 8
    Electronic Resource
    Electronic Resource
    Quantitative analysis of hepatitis C virus activity in vivo in different groups of untreated patients (1997)
    Springer
    Archives of virology 142 (1997), S. 465-472 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Highly sensitive competitive PCR (cPCR) and competitive reverse transcription PCR (cRT-PCR) methodologies were recently developed and applied for quantifying viral DNA and RNA species (including HCV RNA) present in clinical samples at low concentration. In this study, we used cRT-PCR to compare the viral load of 118 untreated patients with HCV infection and different clinical conditions (80 patients with chronic hepatitis, 18 infected subjects with persistently normal ALT levels and various degrees of liver injury, 10 HCV infected subjects that tested positive for anti-LKM1 antibodies, and 10 patients with HCV infection and cryoglobulinemia). The results indicate that while great individual variability of HCV viremia is detectable even among patients with similar clinical conditions, the mean HCV RNA copy number in samples from patients with different clinical conditions was similar in all groups with the single exception of patients that tested positive for anti-liver-kidney microsomal auto-antibodies type 1 (anti-LKM1); interestingly, lower HCV viremia levels were revealed in these anti-LKM1-positive cases with liver disease of uncertain pathogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050093
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  • 9
    Electronic Resource
    Electronic Resource
    Phytoplankton and particulate matter at the Weddell / Scotia Confluence (47°W) in summer 1989, as a final step of a temporal succession (EPOS project) (1997)
    Springer
    Polar biology 18 (1997), S. 1-9 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During January 1989, phytoplankton biomass and species composition were studied in a north / south transect at the Weddell / Scotia Confluence (47°W), between 57° and 61°30′S. Results showed a diatom bloom in the Scotia Sea (chlorophyll a 1.9 μg l−1, particulate organic carbon 239 μg l−1), dominated by Fragilariopsis cylindrus, Dactyliosolen antarcticus and Chaetoceros dichaeta. Low chlorophyll a / phaeopigments ratios (about 1.4) and silicate concentrations (15 μmol l−1) suggested that this was an advanced bloom phase, probably linked to high grazing pressure. Minimum chlorophyll a values of 0.1–0.2 μg l−1 and particulate organic carbon 46 μg l−1 were found at the Weddell / Scotia Front and in a subsurface layer of the Weddell Sea Water. In the southern part of the transect (61°30′S), in the Weddell Sea, a second surface maximum was found (chlorophyll a 0.9 μg l−1, particulate organic carbon 120 μg l−1), but with a different species composition, with Cryptomonas sp. dominant. Our results show a succession within the diatom community in the Weddell / Scotia Confluence Waters when comparing the three EPOS legs. In the Weddell Sea from spring to summer, nanoflagellates, with only a minor contribution from diatoms, persist over a long period with little change in the community structure. We suggest that the frontal system, together with the receding ice edge and the grazing pressure of either krill or protozooplankton, are mainly responsible for the phytoplankton distribution patterns found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003000050152
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  • 10
    Electronic Resource
    Electronic Resource
    Single jet structure ine + e − annihilation (1993)
    Springer
    The European physical journal 58 (1993), S. 71-75 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract By using JETSET 7.2 ase + e − event generator at different c.m. energies, we studiedsingle jet multiplicity distributions in different rapidity andp T intervals. Good NB behavior is found and related clan structure analysis is performed. Observed differences in the behavior of the 2-and 3-jet samples can be understood in terms of the relative contribution of single quark and gluon jet to the 3-jet sample, which are obtained by selecting event by event in this sample the highest and the lowest energy jet respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01554081
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