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Immunization with Attenuated Salmonella typhimurium Producing Catalase in Protection against Gastric Helicobacter pylori Infection in Mice (2003)

Chen, Minhu ; Chen, Jie ; Liao, Wenjun ; [et al.]

Oxford, UK : Blackwell Science Ltd

Helicobacter 8 (2003), S. 0

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ISSN: 1523-5378

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Aim. To evaluate the protective effect of live attenuated Salmonella typhimurium expressing catalase against gastric Helicobacter pylori infection in mice, and to explore the underlying mechanisms of the protective immune reaction.Materials and Methods The H. pylori catalase gene was introduced into attenuated S. typhimurium strain SL3261. C57BL/6 mice were orally immunized with the SL3261 vaccine strain expressing catalase or with SL3261 alone or phosphate-buffered saline (PBS). Mice were sacrificed 4 weeks after immunization and 5 weeks after H. pylori challenge, respectively.Results. All PBS control mice were infected. Eight of 13 (61.5%) mice immunized with the SL3261 vaccine strain and three of 14 (21%) mice immunized with SL3261 alone showed protection against H. pylori infection. Serum anti-H. pylori IgG2a levels of S. typhimurium-immunized mice were higher than those of PBS controls, both before and after H. pylori challenge, while there were no differences for IgG1 and IgA. Similarly, mRNA expression of interleukin (IL)-2, IL-12 and interferon-γ in the gastric mucosa of S. typhimurium-immunized mice was significantly higher than that of PBS controls both before and after challenge. Moreover, S. typhimurium-immunized mice were characterized by marked infiltration of lymphocyte and mononuclear cells in the gastric mucosa after challenge. IL-4 and IL-10 were not detected in any of the three groups. IL-6 expression was increased in the PBS group compared with the S. typhimurium-immunized groups after challenge.Conclusions. This study demonstrates that oral immunization of mice with catalase delivered by an attenuated S. typhimurium strain offers protection against H. pylori infection. This protective immunity was mediated through a predominantly Th1-type response and was associated with post-immunization gastritis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1523-5378.2003.00182.x

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Reduction of the Thrombogenicity of Polyethylene Membranes by Radiation Grafting (2000)

Wu, Minghong ; Bao, Borong ; Chen, Jie

Springer

Journal of radioanalytical and nuclear chemistry 246 (2000), S. 457-461

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ISSN: 1588-2780

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering

Notes: Abstract A new anti-thrombosis dialytic membrane with a hydrophilic-hydrophobic microphase structure was prepared by pre-irradiation grafting of β-hydroxyethyl methacrylate (HEMA) and styrene (St) onto polyethylene (PE) membranes. The effects of reaction conditions on the degree of grafting were determined, and the properties of the grafted films were investigated. Compared with PE grafted with hydrophilic monomer, the antithrombogenicity and permeability of the PE-g-(HEMA-co-St) were 30 and 15 times higher than that of the ungrafted films, respectively, if the volume ratio (HEMA:St) is about 1:1.