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  • 1
    Online Resource
    Online Resource
    Laser Techniques for Fluid Mechanics : Selected Papers from the 10th International Symposium Lisbon, Portugal July 10-13 2000. (2002)
    Berlin, Heidelberg :Springer Berlin / Heidelberg,
    Details
    Keywords: Laser Doppler velocimeter--Congresses. ; Electronic books.
    Type of Medium: Online Resource
    Pages: 1 online resource (555 pages)
    Edition: 1st ed.
    ISBN: 9783662082638
    URL: https://ebookcentral.proquest.com/lib/geomar/detail.action?docID=3099591
    DDC: 620.1064
    Language: English
    Note: Laser Techniques for Fluid Mechanic Selected Papers from the 10th International Symposium, Lisbon, Portugal 10-13 July, 2000 -- Laser Techniques for Fluid Mechanics -- Copyright -- Preface -- The Editors -- Table of Contents -- CHAPTER I. VELOCITY -- I.1. Light-in-flight holography with switched reference beams for cross-correlation in deep volume PIV -- I.2. 3D-PTV experiments of anomalous, steady transport of a conservative tracer in porous media -- I.3. Investigation of the spatio-temporal flow structure in the log-law region of a turbulent boundary layer by means of multi-plane stereo particle image velocimetry -- I.4. 3-Component Doppler laser-two-focus velocimetry applied to a transonic centrifugal compressor -- I.5.Two-point chirp laser Doppler velocimeter using a powerful fibre-coupled green Nd: YAG ring laser -- I.6. Maximum likelihood approaches for sound field measurement using LDV -- CHAPTER II. SCALAR -- II.1. Experimental investigation of heat transfer phenomena during direct contact condensation in the presence of non condensable gas by means of the linear raman spectroscopy -- II.2. On the accuracy of scalar dissipation measurements by laser Rayleigh scatering -- II.3. Demonstration of the applicability of a Background Oriented Schlieren (BOS) method -- II.4. Transient temperature measurement of unburned gas using optic heterodyne interferometry -- CHAPTER III. MULTI-PHASE -- III.1. Generic formulation of a generalized Lorenz-Mie theory for pulsed laser illumination. -- III.2. Measurement and prediction of the gaussian beam effect in the phase Doppler technique -- III.3. Measurement of spray flow by an improved Interferometric Laser Imaging Droplet Sizing (ILIDS) system -- III.4. Two-dimensional imaging of sizes and number densities of nanoscaled particles. , III.5. Simultaneous measurement of velocity and particle size profiles with the reference beam technique -- III.6. Instantaneous whole field measurement of velocity and size of air microbubbles in two-phase flows using DDPIV -- III.7. Liqid PIV measurements around a single gas slug rising through stagnant liquid in vertical pipes -- III.8. PIV applied to landslide generated impulse waves -- CHAPTER IV. TURBULENT FLOWS -- IV.1. Behaviour of trailing vortices in the vicinity of the ground -- IV.2. Investigation of the mixing process in an axisymmetric turbulent jet using PIV and LIF -- IV.3. LDA-measurements of jets in crossflow for effusion cooling applications -- IV.4. Characterisation of confinement and impingement effects on the near field of axisymmetric jets -- IV.5. PIV measurements in co-flowing jets subjected to axial forcing. Vorticity and strain field structure -- IV.6. Laser-optical observation of chaotic mixing structure in a stirred vessel -- IV.7. Stereoscopic PIV measurement of a lobed jet mixing flow -- IV.8. Point and planar LIF for velocity-concentration correlations in a jet in cross flow -- IV.9. Structure of wall-eddies at Reθ ≥ 106 -- CHAPTER V. COMBUSTION AND ENGINES -- V.1. Computer tomography of infra-red absorption and its application to internal-combustion engines -- V.2. A sensor for measuring CO2 gas temperature and concentration using 2µm DFB semiconductor laser -- V.3. Measurement of instantaneous 2-D velocity field and local chemiluminescence in a premixed-spray flame by PIV and MICRO system -- V.4. Phase discrimination inside a spray: LDV measurements using fluorescent seeding particles (FLDV) -- V.5. Effect of strain rate on NOx emission in opposed impinging jet flame combustor -- V.6. Fluid image velocimetry of flow in the recirculation zone of a bluff body stabilized and controlled burner -- Authors' Index.
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  • 2
    Electronic Resource
    Electronic Resource
    Tethering the N-terminus of the prion protein compromises the cellular response to oxidative stress (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 84 (2003), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The role of the N-terminal half of the prion protein (PrPC) in normal cellular function and pathology remains enigmatic. To investigate the biological role of the N-terminus of PrP, we examined the cellular properties of a construct of murine PrP, PrP-DA, in which the N-terminus is tethered to the membrane by an uncleaved signal peptide and which retains the glycosyl-phosphatidylinositol anchor. Human neuroblastoma SH-SY5Y cells expressing PrP-DA were more susceptible to hydrogen peroxide and copper induced toxicity than wtPrP expressing cells. The PrP-DA expressing cells had an increased level of intracellular free radicals and reduced levels of superoxide dismutase and glutathione peroxidase as compared to the wtPrP expressing cells. The membrane topology, cell surface location, lipid raft localisation, intracellular trafficking and copper-mediated endocytosis of PrP-DA were not significantly different from wtPrP. However, cells expressing PrP-DA accumulated an N-terminal fragment that was resistant to proteinase K. The data presented here are consistent with the N-terminal region of PrPC having a role in the cellular response to oxidative stress, and that tethering this region of the protein to the membrane compromises this function through the accumulation of a protease-resistant N-terminal fragment, similar to that seen in some forms of human prion disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2003.01529.x
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  • 3
    Electronic Resource
    Electronic Resource
    The quarternary molecular architecture of TetA, a secondary tetracycline transporter from Escherichia coli (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 38 (2000), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: TetA, a tetracycline cation/proton antiporter, was expressed in Escherichia coli with a C-terminal tag of six histidines, solubilized in dodecyl maltoside and purified in a single step using Ni2+ affinity chromatography. Two-dimensional crystals were obtained after reconstitution of purified protein with lipids. Electron microscopy of negatively stained crystals revealed a trigonal symmetry, from which we infer that this secondary transporter has a trimeric structure. An overall molecular envelope can be described by a triangle of side ≈ 100 Å enclosing a central stain-filled depression. These dimensions are consistent with those obtained from projection views of single, isolated TetA particles that also display a trimeric architecture, confirming that the threefold symmetry is not simply a consequence of crystal-packing interactions. These data represent the first direct view of the quarternary arrangement of any antibiotic efflux pump. They are fully consistent with biochemical data on TetA, which indicate that it functions as a multimer and that the monomer consists of two domains, one of which plays the major part in oligomerization interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.2000.02149.x
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  • 4
    Electronic Resource
    Electronic Resource
    Paediatric lumbar epidurals: a comparison of 21-G and 23-G catheters in patients weighing less than 10 kg (2000)
    Oxford, UK : Blackwell Science Ltd
    Pediatric anesthesia 10 (2000), S. 0 
    Details
    ISSN: 1460-9592
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Using the database of our pain management team, we examined the records of 254 patients weighing between 4.5 kg and 10 kg who received an epidural for postoperative analgesia. We looked at the incidence of catheter related problems in two groups of patients in whom either a 21-G (18-G short Tuohy needle) or a 23-G catheter (19-G short Tuohy needle) was used. There was a significantly higher incidence of difficulty in threading the catheter, kinking and occlusion in the 23-G catheter group. There were more leaks in the 21-G catheter group but the difference was not significant and both groups had a comparable incidence of clinically relevant leakages. No dural tap or difficulty in using either needle were reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-9592.2000.00507.x
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  • 5
    Electronic Resource
    Electronic Resource
    Merkel cell carcinoma and multiple cutaneous squamous cell carcinomas in a patient with pityriasis rubra pilaris (2002)
    Melbourne, Australia : Blackwell Science Asia Pty. Ltd.
    Australasian journal of dermatology 43 (2002), S. 0 
    Details
    ISSN: 1440-0960
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A 79-year-old female was diagnosed with Merkel cell carcinoma (MCC) and multiple cutaneous squamous cell carcinomas (SCC) occurring on a background of pityriasis rubra pilaris. At the time of initial diagnosis and treatment for upper limb MCC, axillary nodal metastases were clinically evident. In the ensuing months, she developed multiple rapidly progressing SCC and eventually a left arm soft tissue deposit of metastatic MCC. Treatment involved multiple courses of fractionated radiotherapy. The salient clinical features and supporting evidence for this case are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1440-0960.2002.00552.x
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  • 6
    Electronic Resource
    Electronic Resource
    Cyclopentenone prostaglandins—new allies in the war on inflammation (2000)
    [s.l.] : Nature America Inc.
    Nature medicine 6 (2000), S. 137-138 
    Details
    ISSN: 1546-170X
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Although the effects of anti-inflammatory drugs have been well studied, little is known about the signaling pathways involved in the inflammatory response. The recent finding that cyclopentenone prostaglandins inhibit IκB kinase provides a new explanation for the anti-inflammatory effects of ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/72224
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  • 7
    Electronic Resource
    Electronic Resource
    Disruption of an SF2/ASF-dependent exonic splicing enhancer in SMN2 causes spinal muscular atrophy in the absence of SMN1 (2002)
    [s.l.] : Nature Publishing Group
    Nature genetics 30 (2002), S. 377-384 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Alteration of correct splicing patterns by disruption of an exonic splicing enhancer may be a frequent mechanism by which point mutations cause genetic diseases. Spinal muscular atrophy results from the lack of functional survival of motor neuron 1 gene (SMN1), even though all affected individuals ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/ng854
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  • 8
    Electronic Resource
    Electronic Resource
    A mechanism for exon skipping caused by nonsense or missense mutations in BRCA1 and other genes (2001)
    [s.l.] : Nature America Inc.
    Nature genetics 27 (2001), S. 55-58 
    Details
    ISSN: 1546-1718
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Point mutations can generate defective and sometimes harmful proteins. The nonsense-mediated mRNA decay (NMD) pathway minimizes the potential damage caused by nonsense mutations. In-frame nonsense codons located at a minimum distance upstream of the last exon-exon junction are recognized as ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/83762
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  • 9
    Electronic Resource
    Electronic Resource
    Analysis of Four Human Microsporidian Isolates by MALDI-TOF Mass Spectrometry (2003)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 50 (2003), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Spores of four species of microsporidia isolated from humans were analyzed by matrix-assisted laser desorption ioni-zation time of flight mass spectrometry (MALDI-TOF MS) and specific biomarkers were found for each. The microsporidia analyzed included three species, Encephalitozoon cuniculi, Encephalitozoon hellem, and Encephalitozoon intestinalis and the fourth organism is the recently described Brachiola algerae. Whole spores, spore shells, and soluble fractions were applied directly to the MALDI target without further purification steps. MALDI-TOF MS analysis of both whole spores and soluble fractions of the four isolates revealed a group of unique, characteristic, and reproducible spectral markers in the mass range of 2,000-8,000 Da. Statistical analysis of the averaged centroided masses uncovered two distinct sets of unique peptides or biomarkers, one originated from whole spores and the other from soluble fractions, that can differentiate the four microsporidian species studied. MALDI-TOF MS analysis of whole organisms is a rapid, sensitive, and specific option to characterize microsporidian isolates and has the potential for several applications in parasi-tology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00110.x
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  • 10
    Electronic Resource
    Electronic Resource
    X-ray study of the orientational order of a concentrated dispersion of kaolinite under flow (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Applied crystallography online 34 (2001), S. 573-579 
    Details
    ISSN: 1600-5767
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences , Physics
    Notes: Diffraction with high-energy X-rays is used to determine the orientational alignment of kaolinite particles dispersed in water as they flow down a pipe. Angle-dispersive and energy-dispersive diffraction methods are compared. Detailed studies of samples as thick as 1 cm were possible using energy-dispersive diffraction. A tomographic technique with the sample scanned and rotated in the beam allows maps of the alignment to be determined. The alignment across a pipe of rectangular section was determined. A strong effect caused by the walls was observed, the plate-like particles tending to lie parallel to the wall near the boundary of the pipe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0021889801009530
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