In:
Yeast, Wiley, Vol. 20, No. 8 ( 2003-06), p. 677-685
Abstract:
The TRP1 gene encoding N‐(5′‐phosphoribosyl)‐anthranilate isomerase was isolated from the yeast Yarrowia lipolytica , in which only a few genetic marker genes are available. The Y. lipolytica TRP1 gene ( YlTRP1 ) cloned by complementation of Y. lipolytica trp1 mutation was found to be a functional homologue of Saccharomyces cerevisiae TRP1 . Since YlTRP1 could be used for counterselection in medium containing 5‐fluoroanthranilic acid (5‐FAA), we constructed TRP blasters that contained YlTRP1 flanked by a direct repeat of a sequence and allowed the recycling of the YlTRP1 marker. Using the TRP blasters the sequential disruption of target genes could be carried out within the same strain of Y. lipolytica . The nucleotide sequence of the YlTRP1 gene has been deposited at GenBank under Accession No. AF420590. Copyright © 2003 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0749-503X
,
1097-0061
Language:
English
Publisher:
Wiley
Publication Date:
2003
detail.hit.zdb_id:
1479172-9
SSG:
12
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