In:
European Journal of Mass Spectrometry, SAGE Publications, Vol. 10, No. 5 ( 2004-10), p. 657-664
Abstract:
Duchenne muscular dystrophy (DMD) is a neuromuscular disease linked to the lack of dystrophin, a submembrane protein, leading to muscle weakness and associated with a defect of the lipid metabolism. A study of the fatty acid composition of glycerophos-phatidylcholines by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) and tandem mass spectrometry (MS/MS) enabled us to characterize a change in the lipid composition of dystrophic cells at the time of the differentiation. This modification has been used as a marker to identify with profiling and imaging MALDI-ToF MS regenerating areas in sections of an mdx mouse leg muscle. It is the first time that such a slight change in fatty acid composition has been observed directly on tissue slices using mass spectrometry. This approach will be useful in monitoring the treatment of muscular regeneration.
Type of Medium:
Online Resource
ISSN:
1469-0667
,
1751-6838
Language:
English
Publisher:
SAGE Publications
Publication Date:
2004
detail.hit.zdb_id:
2021540-X
detail.hit.zdb_id:
2021340-2
SSG:
11
SSG:
12
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