In:
International Journal of Cancer, Wiley, Vol. 97, No. 3 ( 2002-01-20), p. 313-322
Abstract:
Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are well‐known factors that induce neovascularization in many tumors. The molecular mechanisms that regulate tumor angiogenesis in human chondrosarcoma are not clear. We assessed in this work the angiogenic activities of a human chondrosarcoma cell line (OUMS‐27) in vivo and determined the efficacies of angiogenic factors derived from OUMS‐27 cells on human umbilical vein endothelial cells (HUVECs) in vitro . Tumor xenografts induced an increase in the formation of neovessels, but the distributions of Ki‐67 antigen, VEGF and bFGF were unaffected. We also demonstrated that OUMS‐27 cells secreted VEGF 165 into the culture medium and that it was the maximal angiogenic factor to stimulate endothelial proliferation and migration in chondrosarcoma. Anti‐VEGF antibodies induced an approximately 70% inhibition of these responses of HUVECs, but did not have any effect on OUMS‐27 cells. Anti‐bFGF antibodies suppressed not only the activities of HUVECs but also the growth of tumor cells in vitro . We indicate that angiogenesis is principally elicited by VEGF 165 and that tumorigenesis is mainly regulated by bFGF stored in the extracellular matrix of OUMS‐27 cells. The present study may offer the availability of combination therapies for inhibition of VEGF and bFGF action on vascular endothelial cells and chondrosarcoma cells, respectively. © 2001 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0020-7136
,
1097-0215
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
218257-9
detail.hit.zdb_id:
1474822-8
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