In:
Journal of Cellular Biochemistry, Wiley, Vol. 86, No. 2 ( 2002-01), p. 331-339
Abstract:
Induction of heme oxygenase (HO)‐1 during inflammation has been demonstrated in many cell types, but the contribution of inflammatory molecules nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) has remained unresolved. Here we show that NO donors including sodium nitroprusside (SNP) and spermine nonoate (SP‐NO), and PGE 2 significantly stimulate HO‐1 expression in RAW264.7 macrophages, associated with alternative induction on NO and PGE 2 in medium, respectively. NO donors also show the inductive effect on cyclo‐oxygenase 2 protein and PGE 2 production. In the presence of lipopolysaccharide and interferon‐γ (LPS/IFN‐γ), HO‐1 protein was induced slightly but significantly, and SNP, SP‐NO, and PGE 2 enhanced HO‐1 protein induced by LPS/IFN‐γ. L ‐Arginine analogs N ‐nitro‐ L ‐arginine methyl ester ( L ‐NAME) and N ‐nitro‐ L ‐arginine (NLA) significantly block HO‐1 protein induced by LPS/IFN‐γ associated with a decrease in NO (not PGE 2 ) production. And, NSAIDs aspirin and diclofenase dose dependently inhibited LPS/IFN‐γ‐induced HO‐1 protein accompanied by suppression of PGE 2 (not NO) production. PD98059 (a specific inhibitor of MEKK), but not SB203580 (a specific inhibitor of p38 kinase), attenuated PGE 2 (not SP‐NO) induced HO‐1 protein. Under UVC (100 J/m 2 ) and UVB (50 J/m 2 ) irradiation, PGE 2 or SP‐NO treatment prevents cells from UVC or UVB‐induced cell death, and HO‐1 inhibitor tin protoporphyrin (SnPP) reverses the preventive effects of PGE 2 and SP‐NO. The protective activity induced by PGE 2 on UVC or UVB irradiation‐induced cell death was blocked by MAPK inhibitor PD98059 (not SB203580). These results demonstrated that inflammatory molecules NO and PGE 2 were potent inducers of HO‐1 gene, and protected cells from UV‐irradiation‐induced cell death through HO‐1 induction. J. Cell. Biochem. 86: 331–339, 2002. © 2002 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0730-2312
,
1097-4644
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
1479976-5
SSG:
12
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