In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 21 ( 2000-10-10), p. 11198-11202
Abstract:
Overexpression of c-Myc in immortalized cells increases cell
proliferation, inhibits cell differentiation, and promotes cell transformation. Recent evidence suggests that these effects, however,
do not necessarily occur when c-Myc is overexpressed in primary mammalian cells. We sought to determine the immediate effects of
transient overexpression of c-Myc in primary cells in
vivo by using recombinant adenovirus to overexpress human MYC in mouse liver. Mice were intravenously injected
with adenoviruses encoding MYC (Ad/Myc), E2F-1
(Ad/E2F-1), or β-galactosidase (Ad/LacZ). Transgene expression was detectable 4 days after injection. Expression of ectopic c-Myc was
immediately accompanied by enlarged and dysmorphic hepatocytes in the absence of significant cell proliferation or apoptosis. These
findings were not present in the livers of mice injected with Ad/E2F-1 or Ad/LacZ. Prominent hepatocyte nuclei and nucleoli were
associated with the up-regulation of large- and small-subunit ribosomal and nucleolar genes, suggesting that c-Myc may induce their expression
to increase cell mass. Our studies support a role for c-Myc in the in vivo control of vertebrate cell size and metabolism
independent of cell proliferation.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.200372597
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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