In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 22 ( 2000-10-24), p. 12176-12181
Abstract:
We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic
2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related
minichromosomes. The Halobacterium NRC-1 genome codes
for 2,630 predicted proteins, 36% of which are unrelated to any previously reported. Analysis of the genome sequence shows the presence
of pathways for uptake and utilization of amino acids, active sodium-proton antiporter and potassium uptake systems, sophisticated
photosensory and signal transduction pathways, and DNA replication, transcription, and translation systems resembling more complex
eukaryotic organisms. Whole proteome comparisons show the definite archaeal nature of this halophile with additional similarities to the
Gram-positive Bacillus subtilis and other bacteria. The
ease of culturing Halobacterium and the availability of
methods for its genetic manipulation in the laboratory, including construction of gene knockouts and replacements, indicate this
halophile can serve as an excellent model system among the archaea.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.190337797
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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