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  • Artikel  (3)
  • 2000-2004  (3)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    International journal of legal medicine 113 (2000), S. 215-220 
    ISSN: 1437-1596
    Schlagwort(e): Key words FABP ; Troponin ; Myoglobin ; Fibronectin ; Myocardial infarction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract The study was undertaken to evaluate the kinetics and distribution patterns of several immunohistochemical markers in ischemically and hypoxically damaged myocardium. The myocardium of 8 cases of acute myocardial infarction (AMI), 8 cases of diagnosed acute cardiac death (ACD) and 12 cases of acute exogenic hypoxia (AEH) due to CO poisoning or hanging were analysed for depletion of the cardiac antigens FABP, troponin C and T, desmin and myoglobin, loss of CD59 and deposition of the plasma antigens fibrinogen, fibronectin and the terminal complement complex C5b-9. The visualisation of the terminal complement complex was positive as early as 30 min after onset of symptoms of AMI. Depletion of cellular antigens started earlier than the deposition of plasma antigens. The deposition of fibronectin and fibrinogen began earlier than the detection of C5b-9 but later than the depletion of the cellular antigens. Our findings indicate that for the immunohistochemical detection of very early myocardial damage, the depletion of myoglobin is at least of the same rank or better than depletion of FABP and troponin.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    International journal of legal medicine 114 (2000), S. 50-55 
    ISSN: 1437-1596
    Schlagwort(e): Key words C5b-9 ; NP57 ; Putrefaction ; Autolysis ; Myocardial infarction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract Samples of heart tissue were investigated in two series for the detectability of myocardial necrosis after artificial and natural putrefaction, respectively. In the first series heart tissue with and without infarction was artificially subjected to humid and dry autolysis and putrefaction. In the second investigation heart tissue was obtained from exhumed bodies after periods of burial ranging between 10 and 929 days. Besides histology a variety of immunohistochemical markers were applied and C5b-9 gave positive results even after long periods of artificial and natural putrefaction. From the methods tested, this was by far the most sensitive method with a high robustness against putrefaction. NP57, which indicates neutrophilic leucocytes could be demonstrated considerably longer after humid putrefaction than after dry putrefaction. The time limits of detection were considerably longer than for H & E. These two methods are the methods of choice for the detection of myocardial infarction and leucocyte infiltration in advanced stages of putrefaction.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Rechtsmedizin 10 (2000), S. 51-55 
    ISSN: 1434-5196
    Schlagwort(e): Schlüsselwörter MtDNA ; PCR ; DNA-Sequenzierung ; Rechtsmedizinische Spurenkunde ; Key words MtDNA ; PCR ; DNA sequencing ; Forensic science
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Beschreibung / Inhaltsverzeichnis: Abstract Over the last decade mtDNA analysis has gained high importance for forensic identification casework, archeological research as well as for evolutionary and population genetic studies. The structure of the mitochondrial DNA is described with particular consideration of the non-coding hypervariable regions. Furthermore the importance of ¶sequence polymorphisms for evolution biological investigations is discussed. The forensic value of the size and the stucture of sequence databases is underlined. Experimental studies dealing with mtDNA analysis of extracts from teeth, hair shafts and contaminated evidential material are presented. Finally three forensic criminal cases solved by mtDNA analysis are reported.
    Notizen: Zusammenfassung Die Analyse mitochondrialer DNA fand während der letzten Dekade zunehmend Anwendung in der rechtsmedizinischen Praxis, in der Archäologie sowie in evolutions- und populationsgenetischen Studien. Die Struktur der mitochondrialen DNA unter besonderer Berücksichtigung der nichtkodierenden hypervariablen Regionen wird beschrieben. Ferner wird die Bedeutung der Sequenzpolymorphismen für evolutionsbiologische Untersuchungen erörtert. Der forensische Wert von Sequenzdatenbanken sowie Anforderungen an deren Umfang und deren Struktur werden erläutert. Ergebnisse experimenteller Arbeiten mit dem Ziel der Gewinnung mitochondrialer DNA aus bodengelagerten Zähnen, Haarschäften sowie kontaminierten Spuren werden dargestellt. Schließlich werden drei Spurenfälle vorgestellt, die mit Hilfe der mtDNA Analyse geklärt werden konnten.
    Materialart: Digitale Medien
    Standort Signatur Einschränkungen Verfügbarkeit
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