In:
Genomics, Proteomics & Bioinformatics, Oxford University Press (OUP), Vol. 1, No. 2 ( 2003-05-01), p. 101-107
Abstract:
We report a complete genomic sequence of rare isolates (minor genotype) of the SARS-CoV from SARS patients in Guangdong, China, where the first few cases emerged. The most striking discovery from the isolate is an extra 29-nucleotide sequence located at the nucleotide positions between 27,863 and 27,864 (referred to the complete sequence of BJ01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream of the N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29, suggests a significant genetic event and differentiates it from the previously reported genotype, the dominant form among all sequenced SARS-CoV isolates. A 17-nt segment of this extra sequence is identical to a segment of the same size in two human mRNA sequences that may interfere with viral genome replication and transcription in the cytosol of the infected cells. It provides a new avenue for the exploration of the virus-host interaction in viral evolution, host pathogenesis, and vaccine development.
Type of Medium:
Online Resource
ISSN:
1672-0229
,
2210-3244
DOI:
10.1016/S1672-0229(03)01014-3
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2003
detail.hit.zdb_id:
2233708-8
detail.hit.zdb_id:
2240213-5
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