ISSN:
1432-1440
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Description / Table of Contents:
Summary For quantitative determination of fecal protein losses the following procedure was employed: The feces were homogenized in a blender. Depending on th consistency of the feces, especially in normal persons water had to be added in a ratio 2:1 or 1:1 (w/w). An aliquot was centrifuged in a high speed centrifuge (Servall SS1) at 16000 rpm for 5 hours. The supernatant was filtered. The proteins of 1–2 ml of this fluid were precipitated with ethanol (end concentration 80%). After repeated washings of this precipitate with ethanol, the N-content was determined by a Mikrokjehldahl-method. Another aliquot of the original mixture was freeze dried, thus determining the dry wight and the whole fluid content of the feces vice verse. From these data, the daily protein losses were calculated. 10 normal persons had a mean protein loss of 0,51 g a day. (range 0.19–0.85 g). In patients with ulcerative colitis or enterocolitis this figures went up to 13,7 g/24 hours. One patient with protein loosing enteropathy due to intestinal lymphangiectasis excreted between 6 and 36 g proteins a day.
Notes:
Zusammenfassung Es wird eine Methode zur quantitativen Bestimmung der in den Faeces ausgeschiedenen Proteine beschrieben. Mit dieser Methode fand man bei zehn darmgesunden Versuchspersonen eine Proteinausscheidung von 0,19–0,85, im Mittel 0,51 g/24 h. Vier Patienten mit Colitis ulcerosa schieden demgegenüber zwischen 3,2 und 13,7 g Protein/24 h, ein Patient mit enteralem Proteinverlust infolge intestinaler Lymphangiektasie sogar zwischen 6,2 und 35 g/Protein/24 h aus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01727821
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