In:
Proceedings of the Royal Society B: Biological Sciences, The Royal Society, Vol. 276, No. 1676 ( 2009-12-07), p. 4149-4154
Abstract:
This study uses molecular and cytogenetic methods to determine the origin of a B chromosome in some males of the wasp Trichogramma kaykai . This so-called paternal sex ratio (PSR) chromosome transmits only through sperm and shortly after fertilization triggers degeneration of the paternal genome, while keeping itself intact. The resulting embryos develop into haploid B-chromosome-carrying males. Another PSR chromosome with a very similar mode of action is found in the distantly related wasp Nasonia vitripennis and its origin was traced by transposon similarity to the genus Trichomalopsis , which is closely related to Nasonia . To determine whether both PSR chromosomes have a similar origin we aimed to reveal the origin of the Trichogramma PSR chromosome. Using fluorescent in situ hybridization, we discovered a major satellite repeat on the PSR chromosome, the 45S ribosomal DNA. Analysis of the internal transcribed spacer 2 (ITS2) of this repeat showed the presence of multiple ITS2 sequences on the PSR chromosome resembling either the ITS2 of T. oleae or of T. kaykai . We therefore conclude that the Trichogramma PSR chromosome originates from T. oleae or a T. oleae -like species. Our results are consistent with different origins for the PSR chromosomes in Trichogramma and Nasonia .
Type of Medium:
Online Resource
ISSN:
0962-8452
,
1471-2954
DOI:
10.1098/rspb.2009.1238
Language:
English
Publisher:
The Royal Society
Publication Date:
2009
detail.hit.zdb_id:
1460975-7
SSG:
12
SSG:
25
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