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  • 2005-2009  (326)
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  • 2005-2009  (326)
Year
  • 1
    Online Resource
    Online Resource
    Elsevier BV ; 2008
    In:  Materials Research Bulletin Vol. 43, No. 8-9 ( 2008-08), p. 2397-2402
    In: Materials Research Bulletin, Elsevier BV, Vol. 43, No. 8-9 ( 2008-08), p. 2397-2402
    Type of Medium: Online Resource
    ISSN: 0025-5408
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2008
    detail.hit.zdb_id: 1491970-9
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  • 2
    Online Resource
    Online Resource
    Oxford University Press (OUP) ; 2006
    In:  The Plant Cell Vol. 18, No. 2 ( 2006-02-01), p. 412-421
    In: The Plant Cell, Oxford University Press (OUP), Vol. 18, No. 2 ( 2006-02-01), p. 412-421
    Abstract: In this study, we reveal a mechanism by which plants regulate inorganic phosphate (Pi) homeostasis to adapt to environmental changes in Pi availability. This mechanism involves the suppression of a ubiquitin-conjugating E2 enzyme by a specific microRNA, miR399. Upon Pi starvation, the miR399 is upregulated and its target gene, a ubiquitin-conjugating E2 enzyme, is downregulated in Arabidopsis thaliana. Accumulation of the E2 transcript is suppressed in transgenic Arabidopsis overexpressing miR399. Transgenic plants accumulated five to six times the normal Pi level in shoots and displayed Pi toxicity symptoms that were phenocopied by a loss-of-function E2 mutant. Pi toxicity was caused by increased Pi uptake and by translocation of Pi from roots to shoots and retention of Pi in the shoots. Moreover, unlike wild-type plants, in which Pi in old leaves was readily retranslocated to other developing young tissues, remobilization of Pi in miR399-overexpressing plants was impaired. These results provide evidence that miRNA controls Pi homeostasis by regulating the expression of a component of the proteolysis machinery in plants.
    Type of Medium: Online Resource
    ISSN: 1532-298X
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2006
    detail.hit.zdb_id: 623171-8
    detail.hit.zdb_id: 2004373-9
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Springer Science and Business Media LLC ; 2008
    In:  BMC Evolutionary Biology Vol. 8, No. 1 ( 2008-12)
    In: BMC Evolutionary Biology, Springer Science and Business Media LLC, Vol. 8, No. 1 ( 2008-12)
    Abstract: Various expansions or contractions of inverted repeats (IRs) in chloroplast genomes led to fluxes in the IR-LSC (large single copy) junctions. Previous studies revealed that some monocot IRs contain a trnH-rps19 gene cluster, and it has been speculated that this may be an evidence of a duplication event prior to the divergence of monocot lineages. Therefore, we compared the organizations of genes flanking two IR-LSC junctions in 123 angiosperm representatives to uncover the evolutionary dynamics of IR-LSC junctions in basal angiosperms and monocots. Results The organizations of genes flanking IR-LSC junctions in angiosperms can be classified into three types. Generally each IR of monocots contains a trnH-rps19 gene cluster near the IR-LSC junctions, which differs from those in non-monocot angiosperms. Moreover, IRs expanded more progressively in monocots than in non-monocot angiosperms. IR-LSC junctions commonly occurred at polyA tract or A-rich regions in angiosperms. Our RT-PCR assays indicate that in monocot IR A the trnH-rps19 gene cluster is regulated by two opposing promoters, S10 A and psbA . Conclusion Two hypotheses are proposed to account for the evolution of IR expansions in monocots. Based on our observations, the inclusion of a trnH-rps19 cluster in majority of monocot IRs could be reasonably explained by the hypothesis that a DSB event first occurred at IR B and led to the expansion of IRs to trnH , followed by a successive DSB event within IR A and lead to the expansion of IRs to rps19 or to rpl22 so far. This implies that the duplication of trnH-rps19 gene cluster was prior to the diversification of extant monocot lineages. The duplicated trnH genes in the IR B of most monocots and non-monocot angiosperms have distinct fates, which are likely regulated by different expression levels of S10 A and S10 B promoters. Further study is needed to unravel the evolutionary significance of IR expansion in more recently diverged monocots.
    Type of Medium: Online Resource
    ISSN: 1471-2148
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2008
    detail.hit.zdb_id: 2041493-6
    detail.hit.zdb_id: 3053924-9
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Elsevier BV ; 2008
    In:  Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Vol. 69, No. 2 ( 2008-2), p. 664-669
    In: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Elsevier BV, Vol. 69, No. 2 ( 2008-2), p. 664-669
    Type of Medium: Online Resource
    ISSN: 1386-1425
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2008
    detail.hit.zdb_id: 2016492-0
    SSG: 11
    SSG: 21
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  • 5
    Online Resource
    Online Resource
    Oxford University Press (OUP) ; 2005
    In:  Plant Physiology Vol. 137, No. 3 ( 2005-03-01), p. 1037-1048
    In: Plant Physiology, Oxford University Press (OUP), Vol. 137, No. 3 ( 2005-03-01), p. 1037-1048
    Abstract: Vernalization is required to induce flowering in cabbage (Brassica oleracea var Capitata L.). Since FLOWERING LOCUS C (FLC) was identified as a major repressor of flowering in the vernalization pathway in Arabidopsis (Arabidopsis thaliana), two homologs of AtFLC, BoFLC3-2 and BoFLC4-1, were isolated from cabbage to investigate the molecular mechanism of vernalization in cabbage flowering. In addition to the sequence homology, the genomic organization of cabbage FLC is similar to that of AtFLC, except that BoFLC has a relatively smaller intron 1 compared to that of AtFLC. A vernalization-mediated decrease in FLC transcript level was correlated with an increase in FT transcript level in the apex of cabbage. This observation is in agreement with the down-regulation of FT by FLC in Arabidopsis. Yet, unlike that in Arabidopsis, the accumulation of cabbage FLC transcript decreased after cold treatment of leafy plants but not imbibed seeds, which is consistent with the promotion of cabbage flowering by vernalizing adult plants rather than seeds. To further dissect the different regulation of FLC expression between seed-vernalization-responsive species (e.g. Arabidopsis) and plant-vernalization-responsive species (e.g. cabbage), the pBoFLC4-1∷BoFLC4-1∷GUS construct was introduced into Arabidopsis to examine its vernalization response. Down-regulation of the BoFLC4-1∷GUS construct by seed vernalization was unstable and incomplete; in addition, the expression of BoFLC4-1∷GUS was not suppressed by vernalization of transgenic rosette-stage Arabidopsis plants. We propose a hypothesis to illustrate the distinct mechanism by which vernalization regulates the expression of FLC in cabbage and Arabidopsis.
    Type of Medium: Online Resource
    ISSN: 1532-2548 , 0032-0889
    RVK:
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2005
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Oxford University Press (OUP) ; 2006
    In:  Plant Physiology Vol. 141, No. 3 ( 2006-07-01), p. 1000-1011
    In: Plant Physiology, Oxford University Press (OUP), Vol. 141, No. 3 ( 2006-07-01), p. 1000-1011
    Abstract: We recently demonstrated that microRNA399 (miR399) controls inorganic phosphate (Pi) homeostasis by regulating the expression of UBC24 encoding a ubiquitin-conjugating E2 enzyme in Arabidopsis (Arabidopsis thaliana). Transgenic plants overexpressing miR399 accumulated excessive Pi in the shoots and displayed Pi toxic symptoms. In this study, we revealed that a previously identified Pi overaccumulator, pho2, is caused by a single nucleotide mutation resulting in early termination within the UBC24 gene. The level of full-length UBC24 mRNA was reduced and no UBC24 protein was detected in the pho2 mutant, whereas up-regulation of miR399 by Pi deficiency was not affected. Several characteristics of Pi toxicity in the pho2 mutant were similar to those in the miR399-overexpressing and UBC24 T-DNA knockout plants: both Pi uptake and translocation of Pi from roots to shoots increased and Pi remobilization within leaves was impaired. These phenotypes of the pho2 mutation could be rescued by introduction of a wild-type copy of UBC24. Kinetic analyses revealed that greater Pi uptake in the pho2 and miR399-overexpressing plants is due to increased Vmax. The transcript level of most PHT1 Pi transporter genes was not significantly altered, except PHT1;8 whose expression was enhanced in Pi-sufficient roots of pho2 and miR399-overexpressing compared with wild-type plants. In addition, changes in the expression of several organelle-specific Pi transporters were noticed, which may be associated with the redistribution of intracellular Pi under excess Pi. Furthermore, miR399 and UBC24 were colocalized in the vascular cylinder. This observation not only provides important insight into the interaction between miR399 and UBC24 mRNA, but also supports their systemic function in Pi translocation and remobilization.
    Type of Medium: Online Resource
    ISSN: 1532-2548 , 0032-0889
    RVK:
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2006
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Georg Thieme Verlag KG ; 2009
    In:  Planta Medica Vol. 75, No. 15 ( 2009-12), p. 1591-1596
    In: Planta Medica, Georg Thieme Verlag KG, Vol. 75, No. 15 ( 2009-12), p. 1591-1596
    Type of Medium: Online Resource
    ISSN: 0032-0943 , 1439-0221
    Language: English
    Publisher: Georg Thieme Verlag KG
    Publication Date: 2009
    detail.hit.zdb_id: 2037089-1
    SSG: 15,3
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  • 8
    In: Phytochemistry, Elsevier BV, Vol. 69, No. 11 ( 2008-08), p. 2200-2204
    Type of Medium: Online Resource
    ISSN: 0031-9422
    RVK:
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2008
    detail.hit.zdb_id: 2000313-4
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Future Medicine Ltd ; 2007
    In:  Pharmacogenomics Vol. 8, No. 6 ( 2007-06), p. 669-673
    In: Pharmacogenomics, Future Medicine Ltd, Vol. 8, No. 6 ( 2007-06), p. 669-673
    Abstract: Vita Genomics, Inc., centered in Taiwan and China, aims to be a premier genomics-based biotechnological and biopharmaceutical company in the Asia–Pacific region. The company focuses on conducting pharmacogenomics research, in vitro diagnosis product development and specialty contract research services in both genomics and pharmacogenomics fields. We are now initiating a drug rescue program designed to resurrect drugs that have failed in the previous clinical trials owing to low efficacies. This program applies pharmacogenomics approaches using biomarkers to screen subsets of patients who may respond better or avoid adverse responses to the test drugs. Vita Genomics, Inc. has envisioned itself as an important player in the healthcare industry offering advanced molecular diagnostic products and services, revolutionizing thedrug-development process and providing pharmacogenomic solutions.
    Type of Medium: Online Resource
    ISSN: 1462-2416 , 1744-8042
    Language: English
    Publisher: Future Medicine Ltd
    Publication Date: 2007
    SSG: 15,3
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  • 10
    Online Resource
    Online Resource
    Optica Publishing Group ; 2009
    In:  Applied Optics Vol. 48, No. 26 ( 2009-09-10), p. 4942-
    In: Applied Optics, Optica Publishing Group, Vol. 48, No. 26 ( 2009-09-10), p. 4942-
    Type of Medium: Online Resource
    ISSN: 0003-6935 , 1539-4522
    Language: English
    Publisher: Optica Publishing Group
    Publication Date: 2009
    detail.hit.zdb_id: 207387-0
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