In:
Journal of Dairy Research, Cambridge University Press (CUP), Vol. 75, No. 3 ( 2008-08), p. 257-261
Abstract:
Using an ultrafiltration membrane (molecular cut-off, 3000), low molecular weight compounds in bovine milk were collected (YM-3 filtrate). A hydrogen peroxide (H 2 O 2 )-like substance was generated in the YM-3 filtrate. This substance was undetected at 0 h, but increased in a time-dependent manner, peaking after 2 h of incubation at 38°C. After incubating the YM-3 filtrate with catalase and lactoperoxidase, the signal showing the presence of this substance disappeared. The substance was quantified using one chemiluminescence and three colorimetric H 2 O 2 detection systems. In all systems, their estimates were within the same range. The amount of substance, as estimated by the chemiluminescence H 2 O 2 detection system, was correlated with that estimated by the other three colorimetric systems ( r =0·98, 0·95 and 0·87). The substance was eluted at the same position as H 2 O 2 by gel filtration on Superdex 30. Thus, the substance had the same characteristics as H 2 O 2 . An H 2 O 2 -generating substance in either the YM-3 filtrate or whey had a molecular mass of about 600. In this study, we clarify that bovine milk is capable of generating H 2 O 2 by utilizing a low molecular weight compound. Thus, we present a new type of H 2 O 2 -supplying system in bovine milk.
Type of Medium:
Online Resource
ISSN:
0022-0299
,
1469-7629
DOI:
10.1017/S0022029908003154
Language:
English
Publisher:
Cambridge University Press (CUP)
Publication Date:
2008
detail.hit.zdb_id:
2000010-8
SSG:
22
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