In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 144, No. 4 ( 2007), p. 267-274
Abstract:
〈 i 〉 Background: 〈 /i 〉 The 19-kD protein of buckwheat (BW) has been suggested to be a major allergen, but its characteristics and clinical significance are poorly defined. 〈 i 〉 Methods: 〈 /i 〉 cDNA of the 19-kD BW allergen was cloned and expressed in 〈 i 〉 Escherichia coli 〈 /i 〉 . Allergenicity and cross-allergenicity were confirmed by inhibition immunoblotting or by ELISA inhibition. The recombinant (r19-kD) protein was assessed for clinical utility in the diagnosis of BW reactivity in 18 BW-allergic and 19 BW-asymptomatic sensitized subjects using receiver operating characteristic analysis. 〈 i 〉 Results: 〈 /i 〉 The 19-kD BW allergen, which is composed of 135 amino acids, has a weak homology to the vicilin-like allergens of cashew ( 〈 i 〉 Ana 〈 /i 〉 o 1), English walnut ( 〈 i 〉 Jug r 〈 /i 〉 2) and 7 S globulin from 〈 i 〉 Sesamum indicum. 〈 /i 〉 The r19-kD protein can inhibit sIgE binding to native 19-kD BW allergen. The maximum percentage inhibition of sIgE binding to crude BW extract was 56%. About 83.3% of the BW allergy patients had sIgE bound to r19-kD protein, compared to only 1 of the 19 BW-asymptomatic sensitized subjects. The areas under the receiver operating characteristic curves for the skin prick tests [0.925 (95% confidence interval: 0.839–1.012), p 〈 0.001] as well as r19-kD protein sIgE ELISAs [0.860 (95% confidence interval: 0.725–0.995), p 〈 0.001] were higher than that of BW sIgE coated allergen particle test results [0.803 (95% confidence interval: 0.661–0.945), p = 0.002] . 〈 i 〉 Conclusions: 〈 /i 〉 The 19-kD BW allergen may be the major allergen from BW. For the diagnosis of clinical reactivity to BW, the r19-kD protein sIgE ELISA test was more discriminative than the coated allergen particle sIgE measurement using whole BW extract.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
2007
detail.hit.zdb_id:
1482722-0
Permalink