In:
Toxicology and Industrial Health, SAGE Publications, Vol. 25, No. 7 ( 2009-08), p. 473-478
Abstract:
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate, in which the molecular etiology of the defect is poorly characterized. Recently, transforming growth factor-β3 (TGF-β3) has been indicated to play an essential role in the development of palatal shelves. In this developmental toxicity study, we investigated the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of TGF-β3 in fetal mice. Pregnant C57BL/6 mice were exposed to corn oil or TCDD (32 μg/kg/day 64 μg/kg/day, per os) at embryonic day 10 (ED10), a drastic inhibition of palatal shelves was induced. By using RT-PCR (reverse transcription-polymerase chain reaction) and Western blot, the expressions of TGF-β3 was investigated. We found that the expression of TGF-β3 was gradually up-regulated in TCDD-treated group. These results suggest that cleft palate can be induced by TCDD exposure, the modification of TGF-β3 is related to its pathogenesis.
Type of Medium:
Online Resource
ISSN:
0748-2337
,
1477-0393
DOI:
10.1177/0748233709345940
Language:
English
Publisher:
SAGE Publications
Publication Date:
2009
detail.hit.zdb_id:
2010891-6
SSG:
12
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