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  • 2005-2009  (2,591)
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  • 1
    Online Resource
    Online Resource
    2b ORFs encoded by subgroup IB strains of cucumber mosaic virus induce differential virulence on Nicotiana species
    Microbiology Society ; 2007
    In:  Journal of General Virology Vol. 88, No. 9 ( 2007-09-01), p. 2596-2604
    Details
    In: Journal of General Virology, Microbiology Society, Vol. 88, No. 9 ( 2007-09-01), p. 2596-2604
    Abstract: Cucumber mosaic virus (CMV)-encoded 2b protein from subgroup IA or subgroup II was shown to be a determinant of virulence in many solanaceous hosts. In this study, the virulence of 2b proteins from subgroup IB strains was analysed using four intraspecies hybrid viruses, which were generated by precise replacement of the 2b open reading frame (ORF) in subgroup IA strain Fny-CMV with the 2b ORFs of four subgroup IB strains, Cb7-CMV, PGs-CMV, Rad35-CMV and Na-CMV, generating FCb7 2b -CMV, FPGs 2b -CMV, FRad35 2b -CMV and FNa 2b -CMV, respectively. FCb7 2b -CMV was more virulent than Fny-CMV, and was similar in phenotype to its parental virus Cb7-CMV on the three Nicotiana species tested. FNa 2b -CMV also was virulent on these host species, equivalent to Fny-CMV or Na-CMV. However, FRad35 2b -CMV only caused mild mosaic or undetectable symptoms on all the host species tested, and was less virulent than Fny-CMV or Rad35-CMV. FPGs 2b -CMV infected all the host species systemically, and induced either mosaic or barely visible symptoms, demonstrating that the inability of PGs-CMV to infect these three Nicotiana species was not due to its 2b protein. The diverse virulence was shown to be mediated by the 2b proteins rather than the C-terminal overlapping parts of the 2a proteins, and was associated with the level of viral progeny RNA accumulation in systemically infected leaves, but not with the rate of long-distance viral movement in host plants. Through analysis of encapsidation of viral RNAs, there was an apparent correlation between the virulence and the high level of encapsidated RNA 2 in virions of Fny-CMV, FCb7 2b -CMV and FNa 2b -CMV.
    Type of Medium: Online Resource
    ISSN: 0022-1317 , 1465-2099
    URL: Article
    DOI: 10.1099/vir.0.82927-0
    RVK:
    XA 53770
    RVK:
    WA 15000
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2007
    detail.hit.zdb_id: 2007065-2
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Size Consistency Study on the Single Reference Perturbation Theory with Two Hamiltonian Partitions
    Acta Physico-Chimica Sinica & University Chemistry Editorial Office, Peking University ; 2006
    In:  Acta Physico-Chimica Sinica Vol. 22, No. 12 ( 2006), p. 1547-1550
    Details
    In: Acta Physico-Chimica Sinica, Acta Physico-Chimica Sinica & University Chemistry Editorial Office, Peking University, Vol. 22, No. 12 ( 2006), p. 1547-1550
    Type of Medium: Online Resource
    ISSN: 1000-6818
    URL: Article
    DOI: 10.3866/PKU.WHXB20061222
    Language: English
    Publisher: Acta Physico-Chimica Sinica & University Chemistry Editorial Office, Peking University
    Publication Date: 2006
    detail.hit.zdb_id: 2474339-2
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  • 3
    Online Resource
    Online Resource
    A Snapshot of the Emerging Tomato Genome Sequence
    Wiley ; 2009
    In:  The Plant Genome Vol. 2, No. 1 ( 2009-03)
    Details
    In: The Plant Genome, Wiley, Vol. 2, No. 1 ( 2009-03)
    Abstract: The genome of tomato ( Solanum lycopersicum L.) is being sequenced by an international consortium of 10 countries (Korea, China, the United Kingdom, India, the Netherlands, France, Japan, Spain, Italy, and the United States) as part of the larger “International Solanaceae Genome Project (SOL): Systems Approach to Diversity and Adaptation” initiative. The tomato genome sequencing project uses an ordered bacterial artificial chromosome (BAC) approach to generate a high‐quality tomato euchromatic genome sequence for use as a reference genome for the Solanaceae and euasterids. Sequence is deposited at GenBank and at the SOL Genomics Network (SGN). Currently, there are around 1000 BACs finished or in progress, representing more than a third of the projected euchromatic portion of the genome. An annotation effort is also underway by the International Tomato Annotation Group. The expected number of genes in the euchromatin is ∼40,000, based on an estimate from a preliminary annotation of 11% of finished sequence. Here, we present this first snapshot of the emerging tomato genome and its annotation, a short comparison with potato ( Solanum tuberosum L.) sequence data, and the tools available for the researchers to exploit this new resource are also presented. In the future, whole‐genome shotgun techniques will be combined with the BAC‐by‐BAC approach to cover the entire tomato genome. The high‐quality reference euchromatic tomato sequence is expected to be near completion by 2010.
    Type of Medium: Online Resource
    ISSN: 1940-3372 , 1940-3372
    URL: Article
    DOI: 10.3835/plantgenome2008.08.0005
    Language: English
    Publisher: Wiley
    Publication Date: 2009
    detail.hit.zdb_id: 2440458-5
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  • 4
    Online Resource
    Online Resource
    Partially Matched Related Donor Transplantation Can Achieve Outcomes Comparable to Unrelated Donor Transplantation for Patients with Hematologic Malignancies.
    American Society of Hematology ; 2009
    In:  Blood Vol. 114, No. 22 ( 2009-11-20), p. 1197-1197
    Details
    In: Blood, American Society of Hematology, Vol. 114, No. 22 ( 2009-11-20), p. 1197-1197
    Abstract: Abstract 1197 Poster Board I-219 Abstract Purpose: To compare the outcomes of patients undergoing hematopoietic stem cell transplantation (HSCT) from partially matched related donors (PMRDs) or unrelated donors (URDs) for hematologic malignancies without the use of in vitro T cell depletion (TCD). Experimental Design: 297 consecutive patients were performed HSCT from URDs (n = 78) or PMRDs (n = 219) during the same time period. Incidences of graft-versus-host disease (GVHD), relapse, non-relapse mortality (NRM), overall survival (OS) and leukemia-free survival (LFS) are compared between the PMRD and URD groups. Results: All patients achieved full engraftment. The cumulative incidences of grades II to IV acute GVHD in the PMRD and URD cohorts were 47% (95% CI, 33%-62%) versus 31% (CI, 20%-42%, P = .033), with a relative risk (RR) = 1.72 (1.01-2.94), P = .046. The incidence of chronic GVHD did not differ significantly between the two cohorts (P = .17). Two-year incidences of NRM and relapse were 20% (CI, 15%-26%) versus 18% (CI, 10%-27%), with P = 0.98, and 12% (CI, 8%-16%) versus 18% (CI, 10%-27%), with P = .12, for the PMRD versus URD cohort respectively. Four-year OS and LFS were 74% (CI, 67%-80%) versus 74% (CI, 62%-85%), with P = .98, and 67% (CI, 59%-75%) versus 61% (CI, 47%-74%), with P = .74, respectively. Conclusions: Our comparisons demonstrate that every major end point, including relapse, NRM, OS and LFS, was comparable between the PMRD and URD groups. Disclosures: No relevant conflicts of interest to declare.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V114.22.1197.1197
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2009
    detail.hit.zdb_id: 1468538-3
    detail.hit.zdb_id: 80069-7
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  • 5
    Online Resource
    Online Resource
    Partially Matched Related Donor Transplantation Can Achieve Outcomes Comparable with Unrelated Donor Transplantation for Patients with Hematologic Malignancies
    American Association for Cancer Research (AACR) ; 2009
    In:  Clinical Cancer Research Vol. 15, No. 14 ( 2009-07-15), p. 4777-4783
    Details
    In: Clinical Cancer Research, American Association for Cancer Research (AACR), Vol. 15, No. 14 ( 2009-07-15), p. 4777-4783
    Abstract: Purpose: The study aimed to compare the outcomes of patients undergoing hematopoietic stem cell transplantation (HSCT) from partially matched related donors (PMRD) and unrelated donors (URD) for hematologic malignancies without the use of in vitro T cell depletion. Experimental Design: HSCT was done on 297 consecutive patients from URDs (n = 78) and PMRDs (n = 219) during the same time period. Incidences of graft-versus-host disease (GVHD), relapse, nonrelapse mortality, overall survival, and leukemia-free survival between the PMRD and URD groups were compared. Results: All patients achieved full engraftment. The cumct65ulative incidences of grades II to IV acute GVHD in the PMRD and URD cohorts were 47% [95% confidence interval (95% CI), 33-62%] versus 31% (CI, 20-42%; P = 0.033), with a relative risk of 1.72 (95% CI, 1.01-2.94; P = 0.046). The incidence of chronic GVHD did not differ significantly between the two cohorts (P = 0.17). The 2-year incidences of nonrelapse mortality and relapse were 20% (CI, 15-26%) versus 18% (CI, 10-27%), with P = 0.98, and 12% (CI, 8-16%) versus 18% (CI, 10-27%), with P = 0.12, for the PMRD versus the URD cohort, respectively. The 4-year overall survival and leukemia-free survival were 74% (CI, 67-80%) versus 74% (CI, 62-85%), with P = 0.98, and 67% (CI, 59-75%) versus 61% (CI, 47-74%), with P = 0.74, respectively. Conclusions: Our comparisons show that every major end point, including relapse, nonrelapse mortality, overall survival, and leukemia-free survival, was comparable between the PMRD and the URD groups.
    Type of Medium: Online Resource
    ISSN: 1078-0432 , 1557-3265
    URL: Article
    DOI: 10.1158/1078-0432.CCR-09-0691
    RVK:
    XA 36791
    Language: English
    Publisher: American Association for Cancer Research (AACR)
    Publication Date: 2009
    detail.hit.zdb_id: 1225457-5
    detail.hit.zdb_id: 2036787-9
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  • 6
    Online Resource
    Online Resource
    Gastric distention enhances FOS and calcitonin gene‐related peptide expression in the spinal cord and brain of rats
    Wiley ; 2006
    In:  Chinese Journal of Digestive Diseases Vol. 7, No. 1 ( 2006-01), p. 19-23
    Details
    In: Chinese Journal of Digestive Diseases, Wiley, Vol. 7, No. 1 ( 2006-01), p. 19-23
    Abstract: OBJECTIVE:  The purpose of this study was to determine the pathway and mode of transmission of visceral stimuli by investigating the distribution of the FOS and calcitonin gene‐related peptide (CGRP) proteins in the central nervous system. METHODS:  Twenty‐four Sprague‐Dawley rats were divided into three groups: study group ( n  = 12), sham control group ( n  = 6), and normal control group ( n  = 6). A balloon was implanted into the stomach of the rats in the study and sham control groups. After 48 h, the rats in the study group had the stomach distended (80 mmHg) for 2 h, after which they were killed and the antrum, thoracic spinal cord and brain were isolated or dissected. The expression of Fos and CGRP in these tissues was detected immunohistochemically. RESULTS:  FOS expression in the dorsal horn of the spinal cord, dorsal nucleus of the vagal nerve, nucleus of the solitary tract in the study rats was significantly higher than in the sham and normal controls. However, no difference was found between the three groups in FOS expression in the myenteric plexus. Similarly, gastric distention enhanced CGRP expression significantly in the spinal cord and medulla oblongata and correlated closely with FOS expression in these two areas. CONCLUSIONS:  Gastric distention can activate the limbic system, and CGRP plays an important role in the input of visceral stimuli.
    Type of Medium: Online Resource
    ISSN: 1443-9611 , 1443-9573
    URL: Issue
    URL: Article
    DOI: 10.1111/cdd.2006.7.issue-1
    DOI: 10.1111/j.1443-9573.2006.00239.x
    Language: English
    Publisher: Wiley
    Publication Date: 2006
    detail.hit.zdb_id: 2034220-2
    detail.hit.zdb_id: 2317117-0
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  • 7
    Online Resource
    Online Resource
    Key technologies and system design for various radio over fiber applications
    Elsevier BV ; 2009
    In:  The Journal of China Universities of Posts and Telecommunications Vol. 16 ( 2009-9), p. 29-34
    Details
    In: The Journal of China Universities of Posts and Telecommunications, Elsevier BV, Vol. 16 ( 2009-9), p. 29-34
    Type of Medium: Online Resource
    ISSN: 1005-8885
    URL: Article
    DOI: 10.1016/S1005-8885(08)60362-3
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2009
    detail.hit.zdb_id: 2365040-0
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  • 8
    Online Resource
    Online Resource
    Spin-glass state in hexagonal Li2.5Co0.5N
    Elsevier BV ; 2009
    In:  Materials Letters Vol. 63, No. 1 ( 2009-1), p. 133-135
    Details
    In: Materials Letters, Elsevier BV, Vol. 63, No. 1 ( 2009-1), p. 133-135
    Type of Medium: Online Resource
    ISSN: 0167-577X
    URL: Article
    DOI: 10.1016/j.matlet.2008.09.035
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2009
    detail.hit.zdb_id: 1491964-3
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  • 9
    Online Resource
    Online Resource
    Spin-glass-like behavior in rhombohedral Li(Mn,Cr)O2
    AIP Publishing ; 2007
    In:  Journal of Applied Physics Vol. 102, No. 11 ( 2007-12-01)
    Details
    In: Journal of Applied Physics, AIP Publishing, Vol. 102, No. 11 ( 2007-12-01)
    Abstract: The magnetic properties of Cr-doped rhombohedral LiMnO2 are investigated. Two paramagnetic regions are separated at 95K with a reduction in the effective moment due to the change in spin-orbit coupling. Spin-glass-like behavior is suggested at low temperature based on the dc magnetization and magnetic hysteresis measurements.
    Type of Medium: Online Resource
    ISSN: 0021-8979 , 1089-7550
    URL: Article
    DOI: 10.1063/1.2821359
    Language: English
    Publisher: AIP Publishing
    Publication Date: 2007
    detail.hit.zdb_id: 220641-9
    detail.hit.zdb_id: 3112-4
    detail.hit.zdb_id: 1476463-5
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  • 10
    Online Resource
    Online Resource
    Identification of Transcription Factor AP-1 Bound to Untranslated 5′ Regulatory Region DNA of mdr1 Gene with Atomic Force Microscopy.
    American Society of Hematology ; 2006
    In:  Blood Vol. 108, No. 11 ( 2006-11-16), p. 4195-4195
    Details
    In: Blood, American Society of Hematology, Vol. 108, No. 11 ( 2006-11-16), p. 4195-4195
    Abstract: Multidrug resistance(MDR) phenotype of cancer cells is a major obstacle for cancer chemotherapy, this phenotype is main due to the overexprssion of the mdr1 gene. Transcription factor AP-1, which is one of important regulate proteins in the promoter region of mdr1 gene. To date, no direct data of AP-1-DNA regulating complexes on mdr1 gene. A novel method for identifying DNA-binding proteins from image analysis using atomic force microscopy(AFM) was developed. This study is to image and map the structure of Untranslated 5′ regulatory region DNA of mdr1gene, identificate and analysis of transcription factor AP-1 bound to Untranslated 5′ regulatory region DNA of mdr1 gene complex with atomic force microscopy, to find out the molecule mechanism of multidrug resistance. Human leukemia adriamycin resistant strain K562/A02 was used as a target cells, transcription factor AP-1 was used as a target protein. Cultivate and PCR technique was used to amplify K562/A02 cells with the 769bp 5′regulatory region DNA of mdr1 gene, which fragment from −755 to +14. The amplified DNA products were purified by the PCR product purification kit, AP-1 of hela nuclear extract were purified by Sephadex spin-column filled with a bed of Sephadex G-100. The target DNA fragments were incubated with the target protein AP-1 at a 1:2 molar ratio in binding buffer and then immobilized the the AP-1-DNA complexes on a freshly cleaved mica surface which treated by MgCl2. AFM was used to idificate image of the structure of target DNA and the AP-1-DNA complexes. We show here the applicability of AFM in the quantitative analysis of the molecular mechanisms of DNA-protein interaction: The optimum DNA concentration to yield well-absorbed DNA molecular on the mica surface was found to be 10ng/ul. the contour of target DNA AFM image :the length of the DNA fragment measured by AFM image was 260.13± 2.29nm, the width was 11.88 ± 0.92nm, the mean height was 1.2nm(mean±SD, N = 50). Sephadex spin-column (Ultrafree- MC 0.22) can be used to purificate DNA and protein-DNA complex, the contour data of AP-1 protein AFM image:: the width of proteinlarge was 30±3.2nm, protein small was 19±2.8nm; the height of proteinlarge was 3.8±1.4nm, proteinsmall was 3.2±1.8nm (mean±SD, N = 30). we got the contour data of pro- tein-DNA complexes: the width of protein large was 28±2.7nm, the height was 3.4± 0.94nm (mean±SD, N = 8), the width of proteinsmall was 18±1.7nm, the height was 3.1±2.2nm (mean±SD, N = 22), and deduced the possible AP-1 site on mdr1DNA located between bases −126 and −115bp, this result is in close agreement with the expected −121 and −115 bp values. the overall connection efficiency of protein was about 10%. The AFM method to visualize individual biomolecules allows us to investigate the conformation of protein-DNA complexes.
    Type of Medium: Online Resource
    ISSN: 0006-4971 , 1528-0020
    URL: Article
    DOI: 10.1182/blood.V108.11.4195.4195
    RVK:
    XA 33000
    RVK:
    XA 10000
    Language: English
    Publisher: American Society of Hematology
    Publication Date: 2006
    detail.hit.zdb_id: 1468538-3
    detail.hit.zdb_id: 80069-7
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