In:
HortScience, American Society for Horticultural Science, Vol. 40, No. 4 ( 2005-07), p. 1015C-1015
Abstract:
Several plant defense genes, including an iris ribosomal-inactivating protein (I-RIP) and a maize β-glucanase (M-GLU) as well as a small antimicrobial peptide (5 kd) from Mirabilisjalapa (Mj-AMP) were separately introduced into tomato ( Lycopersiconesculentum ) cv. Sweet Chelsea using Agrobacteriumtumefaciens -mediated transformation. Transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using Southern blot hybridization. Transcription of I-RIP, M-GLU , and Mj-AMP genes in various transgenic lines was determined using Northern blot analysis. Plants of some transgenic lines were inoculated with a 2 × 10 4 to 3 × 10 4 conidial spores/mL suspension of the fungal pathogen Alternariasolani , the causal agent of tomato early blight disease. Several transgenic lines carrying either a M-GLU or Mj-AMP transgene showed a higher level of resistance to early blight than control (nontransgenic) plants. The implications of this approach on developing disease resistance in tomato will be discussed.
Type of Medium:
Online Resource
ISSN:
0018-5345
,
2327-9834
DOI:
10.21273/HORTSCI.40.4.1015C
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
2005
detail.hit.zdb_id:
2040198-X
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