In:
Advanced Synthesis & Catalysis, Wiley, Vol. 349, No. 8-9 ( 2007-06-04), p. 1393-1398
Abstract:
The kinetic resolution of a series of acetates of arylaliphatic tertiary alcohols was studied using recombinant esterase variants from Bacillus subtilis (BS2) expressed in E. coli . Highest enantioselectivities (E 〉 100) were achieved in the synthesis of 1,1,1‐trifluoro‐2‐phenylbut‐3‐yn‐2‐ol and three para ‐substituted analogues using BS2 mutant G105A. With mutant E188D only two compounds were converted with E 〉 100. For a thiophene analogue or compounds with small variations in the aliphatic chain substantially lower conversions and/or enantioselectivity were observed, which also varied with the BS2 variant used. Thus, small changes in the substrate structure and point mutations in the esterase had a remarkable influence on both activity and enantioselectivity.
Type of Medium:
Online Resource
ISSN:
1615-4150
,
1615-4169
DOI:
10.1002/adsc.v349:8/9
DOI:
10.1002/adsc.200600641
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
2041384-1
detail.hit.zdb_id:
2033084-4
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