In:
ChemBioChem, Wiley, Vol. 8, No. 5 ( 2007-03-26), p. 513-520
Abstract:
The function of a gramicidin hybrid ion channel in living Chinese hamster ovary (CHO) cells was investigated by the patch clamp method. The synthetic ion channel 1 consists of two cyclohexyl ether amino acids that link two minigramicidin strands. With 1 at a concentration of 1.0 μ M , an increase in the whole‐cell membrane conductance was observed after 1.37 min. The conductance showed larger currents when Cs + was used as charge carrier than when Na + and K + were used. In single‐channel recordings with Cs + as charge carrier, the substance showed comparable single‐channel amplitudes in the membrane of living cells and artificial black lipid bilayers. In addition to functioning as a cation channel, compound 1 appeared to be a water channel. Exposure of the CHO cells to an extracellular hypoosmotic solution did not substantially change the cell volume. Extracellular hypoosmotic conditions in the presence of 1 increased the cell size to 146.5 % that of the control. Thus, the synthetic hybrid channel 1 can function as a cation channel with some Cs + specificity, and as a water channel in CHO cells.
Type of Medium:
Online Resource
ISSN:
1439-4227
,
1439-7633
DOI:
10.1002/cbic.200600384
Language:
English
Publisher:
Wiley
Publication Date:
2007
detail.hit.zdb_id:
2020469-3
SSG:
12
Permalink