In:
American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 66, No. 9 ( 2005-09-01), p. 1584-1589
Abstract:
Objective —To evaluate cytokine production by
equine alveolar macrophages after exposure to lipopolysaccharide (LPS), Aspergillus fumigatus , and
hay dust, and determine the effect of clenbuterol on the cytokine response. Animals —6 horses. Procedures —Alveolar macrophages were exposed to
PBS solution (negative control), LPS, hyphae and conidia of Aspergillus fumigatus (AF), or a suspension of
hay dust (HDS) and incubated for 24 hours at 37°C. Concentrations of tumor necrosis factor (TNF)-α and
interleukin (IL)-1β were measured in the supernatant. The procedure was repeated with cells that were concurrently
incubated with 0.5µM clenbuterol. Results —Exposure to HDS and AF significantly
increased production of TNF-α by equine alveolar macrophages. The increase in TNF-α produced in
response to HDS and AF was 5 and 7 times as great, respectively, as the increase measured in response to
LPS. The concentration of IL-1β in the supernatant was significantly increased after exposure of cells to
AF. Clenbuterol was effective at inhibiting TNF-α production by cells exposed to LPS, HDS, or AF. Conclusions and Clinical Relevance —Increased
production of TNF-α and IL-1 indicated that the proinflammatory cytokines produced by alveolar
macrophages in response to allergens may play a role in recurrent airway obstruction (RAO) in horses.
Equine alveolar macrophages are not only a primary pulmonary defense mechanism but may also influence
the pathogenesis of equine RAO. The β 2 -adrenoceptor
agonist clenbuterol, a drug that is commonly used for treatment of equine RAO, promotes immediate
bronchodilation and may also contribute to downward modulation of the inflammatory response.
( Am J Vet Res 2005;66:1584–1589)
Type of Medium:
Online Resource
ISSN:
0002-9645
DOI:
10.2460/ajvr.2005.66.1584
Language:
Unknown
Publisher:
American Veterinary Medical Association (AVMA)
Publication Date:
2005
detail.hit.zdb_id:
2056942-7
SSG:
22
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