In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 57, No. 7 ( 2007-07-01), p. 1396-1401
Abstract:
Two novel strains belonging to the phylum Bacteroidetes [formerly the Cytophaga – Flexibacter – Bacteroides (CFB) group], designated Gsoil 040 T and Gsoil 052 T , were isolated from the soil of a ginseng field in Pocheon province, South Korea. A polyphasic approach was used to characterize the taxonomic position of the novel strains. Both strains were Gram-negative, aerobic, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the novel isolates belong to the genus Chitinophaga but are clearly separated from the recognized species of this genus; gene sequence similarities between the novel isolates and type strains of recognized species ranged from 91.2 to 96.5 %. One exception was found; strain Gsoil 052 T and the type strain of Chitinophaga filiformis had a gene sequence similarity of 99.6 % but had a DNA–DNA relatedness value of 38 %. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; major fatty acids, iso-C 15 : 0 and C 16 : 1 ω 5 c ; major hydroxy fatty acid, iso-C 17 : 0 3-OH and major polyamine, homospermidine) supported the affiliation of both strains Gsoil 040 T and Gsoil 052 T to the genus Chitinophaga . The results of physiological and biochemical tests enabled the genotypic and phenotypic differentiation of the novel strains from the other recognized species of the genus Chitinophaga . Therefore, it is suggested that the new isolates represent two novel species, for which the names Chitinophaga ginsengisegetis sp. nov. [type strain Gsoil 040 T (=KCTC 12654 T =DSM 18108 T )] and Chitinophaga ginsengisoli sp. nov. [type strain Gsoil 052 T (=KCTC 12592 T =DSM 18017 T )] are proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.64688-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2007
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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