In:
Canadian Journal of Physiology and Pharmacology, Canadian Science Publishing, Vol. 86, No. 12 ( 2008-12), p. 860-865
Kurzfassung:
It is well known that choline has protective effects on ischemic arrhythmias. We designed the present study to evaluate the antiarrhythmic effects of choline and to detect its related mechanisms in aconitine-induced rat and ouabain-induced guinea pig models of arrhythmia. Laser scanning confocal microscopy and patch-clamp technique were utilized to study the action of choline on intracellular calcium concentration and L-type calcium current (I Ca-L ) of cardiac myocytes. M 3 receptor antagonist 4-DAMP (4-diphenylacetoxy-N-methylpiperidine-methiodide) was applied preliminarily to evaluate the role of the M 3 receptor. Choline significantly increased the survival time of arrhythmic rats and guinea pigs, delayed the onset of arrhythmias and ventricular tachycardia, and decreased the arrhythmia score. The overload of intracellular Ca 2+ induced by aconitine or ouabain was reduced in isolated myocytes pretreated with choline. Choline reduced the increased density of I Ca-L induced by aconitine or ouabain. Moreover, the beneficial effects of choline were reversed by 4-DAMP. Choline produced antiarrhythmic actions on arrhythmia models by stimulating the cardiac M 3 receptor. The mechanism may be related to the improvement of Ca 2+ handling.
Materialart:
Online-Ressource
ISSN:
0008-4212
,
1205-7541
Sprache:
Englisch
Verlag:
Canadian Science Publishing
Publikationsdatum:
2008
ZDB Id:
2004356-9
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