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  • Microbiology Society  (13)
  • 2005-2009  (13)
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  • Microbiology Society  (13)
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  • 2005-2009  (13)
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  • 1
    Online Resource
    Online Resource
    Bombyx mori nucleopolyhedrovirus ORF56 encodes an occlusion-derived virus protein and is not essential for budded virus production
    Microbiology Society ; 2008
    In:  Journal of General Virology Vol. 89, No. 5 ( 2008-05-01), p. 1212-1219
    Details
    In: Journal of General Virology, Microbiology Society, Vol. 89, No. 5 ( 2008-05-01), p. 1212-1219
    Abstract: Bombyx mori nucleopolyhedrovirus ORF56 ( Bm56 ) is a baculovirus core gene that is highly conserved in all baculoviruses that have had their genomes sequenced to date. Its transcripts in BmNPV-infected cells could be detected from 12 h post-infection (p.i.) and the encoded protein could be detected at 16 h p.i. by using a polyclonal antibody against glutathione S -transferase–Bm56 fusion protein. Western blot analysis showed that Bm56 is a structural component of the occlusion-derived virus nucleocapsid. Subsequent confocal microscopy revealed that Bm56 was distributed in the outer nuclear membrane and the intranuclear region of infected cells. To investigate the role of Bm56 in virus replication, a Bm56 -knockout bacmid of BmNPV was constructed via homologous recombination in Escherichia coli . The Bm56 deletion had no effect on budded virus (BV) production in cultured cells; however, the deletion affected occlusion-body morphogenesis. A larval bioassay demonstrated that the Bm56 deletion did not reduce infectivity, whereas it resulted in a 50 % lethal time that was 16–18 h longer than that of the wild-type bacmid at every dose used in this study. These results indicate that Bm56 facilitates efficient virus production in vivo ; however, it is not essential for BV production in vitro .
    Type of Medium: Online Resource
    ISSN: 0022-1317 , 1465-2099
    URL: Article
    DOI: 10.1099/vir.0.83633-0
    RVK:
    XA 53770
    RVK:
    WA 15000
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2008
    detail.hit.zdb_id: 2007065-2
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment
    Microbiology Society ; 2006
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 56, No. 2 ( 2006-02-01), p. 439-442
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 56, No. 2 ( 2006-02-01), p. 439-442
    Abstract: An aerobic, yellow-pigmented, Gram-negative bacterium, designated strain S13 T , was isolated from freshwater sediment of Taihu Lake in central China. The taxonomy of strain S13 T was studied by using phenotypic and phylogenetic methods. Cells of strain S13 T were rod-shaped, non-motile and with a size range of 0·35–0·55×1·5–2·5 μm. The nearly complete 16S rRNA gene of strain S13 T was amplified and sequenced. A blast search and phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain S13 T was related to members of the genus Flavobacterium , with the highest sequence similarity of 93·8 % to Flavobacterium columnare (ATCC 23463 T ). Cells contained menaquinone-6 (MK-6) as the major respiratory quinone and the genomic DNA G+C content was 41 mol%. The major fatty acids were iso-C 15 : 0 (28·2 %) and iso-C 17 : 1 ω 9 c (19·0 %). It is proposed that S13 T (=CGMCC 1.3801 T =JCM 13331 T ) represents the type strain of a novel species, Flavobacterium saliperosum sp. nov.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.64065-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2006
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Zhouia amylolytica gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from sediment of the South China Sea
    Microbiology Society ; 2006
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 56, No. 12 ( 2006-12-01), p. 2825-2829
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 56, No. 12 ( 2006-12-01), p. 2825-2829
    Abstract: Three Gram-negative, non-spore-forming strains were isolated from sediment from the South China Sea, China, and their taxonomic positions were investigated using a polyphasic approach. Strains HN-171 T , HN-172 and HN-181 grew optimally at 30 °C, in the presence of 4.5–5.0 NaCl % (w/v) and at pH 7.2–7.4. They contained MK-6 as the predominant respiratory quinone and contained iso-C 15 : 1 G, iso-C 15 : 0 , summed feature 4 (iso-C 15 : 0 2-OH and/or C 16 : 1 ω 7 c / t ) and C 15 : 0 as the major fatty acids. The DNA G+C content of strain HN-171 T was 34.5 mol%. Phylogenetic analyses based on 16S rRNA gene sequences demonstrated that strain HN-171 T , together with strains HN-172 and HN-181, formed a distinct evolutionary lineage within the family Flavobacteriaceae . The 16S rRNA gene sequences of strains HN-171 T , HN-172 and HN-181 shared 99.8–100 % similarity with each other, and the sequence of strain HN-171 T exhibited similarity values below 90.2 % with those of other members of the family Flavobacteriaceae . The closest relative of HN-171 T was Coenonia anatina LMG 14382 T (90.2 %). On the basis of their phenotypic and phylogenetic properties, the three isolates represent a novel genus and a novel species, for which the name Zhouia amylolytica gen. nov., sp. nov. is proposed. The type strain is HN-171 T (=CGMCC 1.6114 T =JCM 14016 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.64587-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2006
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Micrococcus flavus sp. nov., isolated from activated sludge in a bioreactor
    Microbiology Society ; 2007
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 57, No. 1 ( 2007-01-01), p. 66-69
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 57, No. 1 ( 2007-01-01), p. 66-69
    Abstract: Bacterial strain LW4 T was isolated from activated sludge of a wastewater-treatment bioreactor. Cells of strain LW4 T were Gram-positive cocci, with a diameter of 0.7–1.0 μm. Colonies produced on LB agar plates were yellow, smooth, circular and 0.5–1.5 mm in diameter. Strain LW4 T was aerobic and grew over the temperature range 26–34 °C and pH range 5–9, with optimal growth at 30.5–31.5 °C and pH 6.0–6.2. The cell-wall peptidoglycan of strain LW4 T contained amino acid residues of lysine, glutamic acid, alanine, glycine and aspartic acid. The most abundant cellular fatty acids of strain LW4 T were anteiso-C 15 : 0 (32.15 %) and iso-C 15 : 0 (31.65 %). Major respiratory quinones were MK-8(H 2 ) (57.3 %) and MK-7(H 2 ) (32.9 %). The DNA G+C content was 71.4 mol% ( T m ). 16S rRNA gene sequence analysis indicated that strain LW4 T was phylogenetically related to members of the genus Micrococcus , with similarities ranging from 96.5 to 97.3 %. Levels of DNA–DNA relatedness of strain LW4 T to Micrococcus luteus DSM 20030 T , Micrococcus lylae DSM 20315 T and Micrococcus antarcticus AS 1.2372 T were 55, 48 and 36 %, respectively. Based on these results, it is concluded that strain LW4 T represents a novel species of the genus Micrococcus , for which the name Micrococcus flavus sp. nov. is proposed. The type strain is strain LW4 T (=CGMCC 1.5361 T =JCM 14000 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.64489-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2007
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov.
    Microbiology Society ; 2005
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 55, No. 2 ( 2005-03-01), p. 699-702
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 55, No. 2 ( 2005-03-01), p. 699-702
    Abstract: An aerobic, motile, Gram-positive strain, DX3-12 T , was isolated from coastal sediment of the Eastern China Sea in Fujian Province, China, and was identified by means of polyphasic taxonomy. On the basis of 16S rRNA gene sequence similarity, strain DX3-12 T was closely related to members of the genera Planomicrobium and Planococcus . 16S rRNA gene sequence similarities between strain DX3-12 T and Planomicrobium mcmeekinii , Planomicrobium koreense , Planomicrobium okeanokoites , Planococcus psychrophilus and Planococcus alkanoclasticus were 97·8, 97·8, 97·1, 97·5 and 96·4 %, respectively. Chemotaxonomic and physiological properties of strain DX3-12 T supported its affiliation to the genus Planomicrobium . Strain DX3-12 T and Planomicrobium mcmeekinii exhibited common phenotypic properties such as the ability to reduce nitrate, but they could be distinguished from each other by physiological and biochemical tests, e.g. acid production from glucose, growth temperature and NaCl concentration requirements. DNA–DNA hybridization further distinguished strain DX3-12 T from Planomicrobium mcmeekinii at the species level. Therefore, it is proposed that strain DX3-12 T (=AS 1.3454 T =JCM 12466 T ) represents a novel species, Planomicrobium chinense sp. nov. Based on 16S rRNA gene sequence similarities, signature nucleotides, cell morphology and physiological and biochemical properties, Planococcus psychrophilus and Planococcus alkanoclasticus have been transferred to the genus Planomicrobium as Planomicrobium psychrophilum comb. nov. (type strain DSM 14507 T =MTCC 3812 T ) and Planomicrobium alkanoclasticum comb. nov. (type strain NCIMB 13489 T =CIP 107718 T ), respectively.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.63340-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2005
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Asticcacaulis taihuensis sp. nov., a novel stalked bacterium isolated from Taihu Lake, China
    Microbiology Society ; 2005
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 55, No. 3 ( 2005-05-01), p. 1239-1242
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 55, No. 3 ( 2005-05-01), p. 1239-1242
    Abstract: A novel stalked bacterium, designated strain T3-B7 T , was isolated from sediment of Taihu Lake, Jiangsu Province, China, and its taxonomy was studied by using a polyphasic approach. Cell morphology, physiological and biochemical properties, and polar lipids indicated that strain T3-B7 T represented a member of the genus Asticcacaulis . Based on 16S rRNA gene sequence similarity analysis, strain T3-B7 T was found to be phylogenetically related to Asticcacaulis biprosthecium DSM 4723 T (98·5 %) and Asticcacaulis excentricus DSM 4724 T (95·0 %), but could be differentiated from these two species on the basis of the number and position of prosthecae, assimilation of sugars, nitrate reduction and tolerance to NaCl. Levels of DNA–DNA relatedness of strain T3-B7 T to A. biprosthecium DSM 4723 T and A. excentricus DSM 4724 T were 37·1 and 18·0 %, respectively. The G+C content of strain T3-B7 T was 59 mol% ( T m ). It is concluded that strain T3-B7 T represents a novel species of the genus Asticcacaulis , for which the name of Asticcacaulis taihuensis sp. nov. is proposed. The type strain is T3-B7 T (=AS 1.3431 T =JCM 12463 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.63334-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2005
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Sanguibacter marinus sp. nov., isolated from coastal sediment
    Microbiology Society ; 2005
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 55, No. 5 ( 2005-09-01), p. 1755-1758
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 55, No. 5 ( 2005-09-01), p. 1755-1758
    Abstract: A Gram-positive, coryneform bacterium, strain 1-19 T , was isolated from coastal sediment from the Eastern China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that the organism formed a robust clade with the type species of the genus Sanguibacter and displayed less than 97 % gene sequence similarity. Phenotypic characteristics supported the assignment of this organism to the genus Sanguibacter . A range of biochemical features distinguished it from all other Sanguibacter species with validly published names. On the basis of a polyphasic taxonomical analysis, it is proposed that this bacterium is a novel species of Sanguibacter , for which the name Sanguibacter marinus sp. nov. is proposed. The type strain is 1-19 T (=CGMCC 1.3457 T =JCM 12547 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.63471-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2005
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Yangia pacifica gen. nov., sp. nov., a novel member of the Roseobacter clade from coastal sediment of the East China Sea
    Microbiology Society ; 2006
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 56, No. 3 ( 2006-03-01), p. 529-533
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 56, No. 3 ( 2006-03-01), p. 529-533
    Abstract: An aerobic, Gram-negative bacterial isolate, strain DX5-10 T , was isolated from coastal sediment of the East China Sea. The taxonomy of strain DX5-10 T was studied by phenotypic and phylogenetic methods. Strain DX5-10 T was motile, formed faint-yellowish colonies and was positive for catalase reaction and weakly positive for oxidase reaction. The nearly complete 16S rRNA gene of strain DX5-10 T was obtained and sequence analysis indicated that strain DX5-10 T represented an independent lineage within the Roseobacter clade of Alphaproteobacteria . Strain DX5-10 T was phylogenetically related to members of the genera Roseobacter , Loktanella , Roseisalinus , Silicibacter , Antarctobacter , Sulfitobacter , Salipiger , Ruegeria and Roseivivax , and the sequence identities among them were less than 95·0 %. The predominant respiratory ubiquinone of strain DX5-10 T was Q-10 and the DNA G+C content of strain DX5-10 T was 63·3 mol%. Therefore, strain DX5-10 T represents a novel species of a novel genus, for which the name Yangia pacifica gen. nov., sp. nov. is proposed, with the type strain DX5-10 T (=CGMCC 1.3455 T =JCM 12573 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.64013-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2006
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 9
    Online Resource
    Online Resource
    Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter
    Microbiology Society ; 2007
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 57, No. 2 ( 2007-02-01), p. 219-222
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 57, No. 2 ( 2007-02-01), p. 219-222
    Abstract: A novel marine bacterial strain, HY1 T , was isolated from sediment of the South China Sea. The strain was aerobic and heterotrophic and formed saffron yellow-pigmented colonies on marine agar 2216. Cells were non-motile, Gram-negative rods, frequently occurring in chains. blastn searches revealed that the 16S rRNA gene sequence of strain HY1 T showed high similarity with those of members of the genera Gillisia (91.7–93.8 %) and Salegentibacter (92.6–93.5 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain clustered with members of both Salegentibacter and Gillisia and phylogenetic trees constructed using three different methods (neighbour-joining, maximum-parsimony and minimum-evolution) indicated that strain HY1 T clustered more frequently with members of the genus Salegentibacter . The DNA G+C content of strain HY1 T was 44.4 mol% and its major cellular fatty acids (⩾5 % of the total fatty acids) were iso-15 : 1 (5.0 %), iso-15 : 0 (6.8 %), anteiso-15 : 0 (6.4 %), 15 : 0 (10.4 %), iso-16 : 0 (13.5 %), summed feature 3 (comprising iso-15 : 0 2-OH and/or 16 : 1 ω 7 c ; 6.3 %), iso-17 : 0 3-OH (5.2 %) and 17 : 0 2-OH (5.0 %). Cells contained menaquinone 6. Based on the phylogenetic and phenotypic analyses, strain HY1 T should be classified as representing a novel species within the genus Salegentibacter , for which the name Salegentibacter catena sp. nov. is proposed. The type strain is HY1 T (=CGMCC 1.6101 T =JCM 14015 T ). Based on this study and on previously described Salegentibacter species, an emended description of the genus Salegentibacter is given.
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.64658-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2007
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Wenxinia marina gen. nov., sp. nov., a novel member of the Roseobacter clade isolated from oilfield sediments of the South China Sea
    Microbiology Society ; 2007
    In:  International Journal of Systematic and Evolutionary Microbiology Vol. 57, No. 8 ( 2007-08-01), p. 1711-1716
    Details
    In: International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 57, No. 8 ( 2007-08-01), p. 1711-1716
    Abstract: An aerobic and heterotrophic, Gram-negative bacterial isolate, strain HY34 T , was isolated from sediment of an oilfield in the South China Sea, China. The taxonomy of strain HY34 T was studied by phenotypic and phylogenetic methods. Strain HY34 T formed faint-pink colonies on marine agar 2216. Cells of strain HY34 T were non-motile, ovoid or short rods. Strain HY34 T was positive for catalase and oxidase, and nitrate was reduced to nitrite. The nearly complete 16S rRNA gene sequence of strain HY34 T was obtained and sequence analysis showed that it, together with the genus Rubellimicrobium , formed a distinct clade close to some members of the Roseobacter clade in the family Rhodobacteraceae , and it showed highest sequence similarities to Oceanicola granulosus HTCC2516 T (93.8 %), Silicibacter lacuscaerulensis ITI-1157 T (93.3 %), Dinoroseobacter shibae DFL 12 T (93.3 %) and Rubellimicrobium thermophilum C-lvk-R2A-2 T (92.2 %). Bacteriochlorophyll a was not detected. The ubiquinone system was Q-10. The major polar lipids were phosphatidylglycerol, phosphatidylcholine and an unidentified glycolipid. The major fatty acids ( 〉 10 %) were C 18 : 1 ω 7 c and C 16 : 0 . The DNA G+C content of this strain was 69.4 mol%. A polyphasic analysis supported the conclusion that this strain represents a novel genus and species, which we designated Wenxinia marina gen. nov., sp. nov. The type strain of Wenxinia marina is HY34 T (=CGMCC 1.6105 T =JCM 14017 T ).
    Type of Medium: Online Resource
    ISSN: 1466-5026 , 1466-5034
    URL: Article
    DOI: 10.1099/ijs.0.64825-0
    Language: English
    Publisher: Microbiology Society
    Publication Date: 2007
    detail.hit.zdb_id: 215062-1
    detail.hit.zdb_id: 2056611-6
    SSG: 12
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