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  • Cambridge University Press (CUP)  (11)
  • 2005-2009  (11)
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  • Cambridge University Press (CUP)  (11)
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  • 2005-2009  (11)
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  • 1
    Online Resource
    Online Resource
    Cloning of 3′-end full-length cDNA encoding for MT-I and MT-III in yak and analysis of their sequences
    Cambridge University Press (CUP) ; 2009
    In:  Chinese Journal of Agricultural Biotechnology Vol. 6, No. 3 ( 2009-12), p. 207-214
    Details
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 6, No. 3 ( 2009-12), p. 207-214
    Abstract: Domestic yak ( Bos grunnien s) metallothionein-I (MT-I) and metallothionein-III (MT-III) cDNA 3′-end full-length sequences (331 and 378 bp; GenBank accession MT-I no. AY758557, MT-III no. DQ492300) from the total RNA of liver and brain tissues were amplified and cloned by reverse transcription polymerase chain reaction (RT-PCR) and 3′-rapid amplification of cDNA ends (RACE) using the primers Y MT-I SP and M13 primers M4, and Y MT-III SP and M13 primers M4, respectively, which included the MT-I (183 bp) coding sequence, MT-III (207 bp) cDNA coding sequences, and also the tailing signal AATAAA and Poly(A) at the 3′-ends of the MT-I and MT-III, respectively. The analyses showed that the yak cDNA sequence coding for MT-I protein was composed of 61 amino acids, including 20 cysteines having a conserved tripeptide structure, for example C–X–C, C–C–X–C–C, C–X–X–C and so on. The yak cDNA sequence coding for MT-III protein was composed of 68 amino acids, including 19 cysteines having both MT-III-specific conserved tripeptides and the same conserved tripeptides as MT-I, including T, CPCP, GEGAEA and so on. They were all comparatively conservative in molecular evolution. These structures indicated that MT-III had the same physiological functions regarding heavy metals (detoxification and so on) as MT-I, and also different specific functions from MT-I, including inhibition of neuron growth.
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    URL: Article
    DOI: 10.1017/S1479236209990441
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2009
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  • 2
    Online Resource
    Online Resource
    Isolation and sequence analysis of the floral homeotic gene BAP2 in Brassica rapa
    Cambridge University Press (CUP) ; 2006
    In:  Chinese Journal of Agricultural Biotechnology Vol. 3, No. 2 ( 2006-08), p. 141-146
    Details
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 3, No. 2 ( 2006-08), p. 141-146
    Abstract: The Arabidopsis homeotic gene AP2 is essential for floral meristem development and organ specification. In this study, we report the isolation and sequence comparison of the AP2 homologue of Brassica rapa ( BAP2 ). The results showed that the BAP2 DNA sequence was 2138 bp in length and contained nine introns. It shared 90% identity with AP2 in the coding region. The putative BAP2 polypeptide contained a nuclear localization signal (NLS) sequence and two copies of highly conserved AP2 domain, suggesting that BAP2 may function similarly to AP2 . Two nucleotide variations were detected in DNA sequences from wild-type and apetalous B. rapa plants at the BAP2 locus, while the putative polypeptides were identical. We propose that BAP2 is not likely to be responsible for the apetalous mutation in B. rapa .
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    URL: Article
    DOI: 10.1079/CJB2006101
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2006
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  • 3
    Online Resource
    Online Resource
    CRS-PCR polymorphisms of the GHR gene and its relationship with milk production traits in Chinese Holstein cows
    Cambridge University Press (CUP) ; 2009
    In:  Chinese Journal of Agricultural Biotechnology Vol. 6, No. 3 ( 2009-12), p. 215-219
    Details
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 6, No. 3 ( 2009-12), p. 215-219
    Abstract: Single-nucleotide polymorphisms (SNPs) of exon 8 of the GHR gene were detected in Chinese Holstein cows by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The results showed that exon 8 of the GHR gene digested by Tas I could be divided into two kinds of alleles and three kinds of genotypes. The frequencies of allele A and T were 0.6339 and 0.3661, respectively. The frequencies of genotypes AA, AT and TT were 0.459, 0.350 and 0.191, respectively. Sequencing showed one single nucleotide mutation T→A at 4962 bp of the gene in genotype TT when compared with genotype AA, and this mutation resulted in an amino acid change of phenylalanine (TTT)→tyrosine (TAT). The result of χ 2 testing indicated that the genotypic frequency of the GHR gene digested by Tas I did not fit with Hardy–Weinberg equilibrium in this population ( P 〈 0.05). The least-square mean of the fat percentage of this population was higher for genotype TT than for genotype AA ( P 〈 0.05).
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    URL: Article
    DOI: 10.1017/S1479236209990283
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2009
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  • 4
    Online Resource
    Online Resource
    Effect of surfactants on the inertialess instability of a two-layer film flow
    Cambridge University Press (CUP) ; 2007
    In:  Journal of Fluid Mechanics Vol. 591 ( 2007-11-25), p. 495-507
    Details
    In: Journal of Fluid Mechanics, Cambridge University Press (CUP), Vol. 591 ( 2007-11-25), p. 495-507
    Abstract: The effect of insoluble surface and interfacial surfactants on the inertialess instability of a two-fluid film flow down an inclined plane is investigated based on a normal mode analysis. The results reveal that the inertialess instability of relatively long waves can be predominantly weakened by a surface surfactant and enhanced by an interfacial surfactant. For sufficiently large viscosity ratio of the upper layer to the lower one, a destabilizing influence of the surface surfactant is also detected; this is thus a rare example demonstrating the possible destabilizing effect of the surfactant on the flow with a free surface. When the upper layer is less viscous and hence the instability due to the viscosity stratification disappears, a new instability can be triggered by the presence of an interfacial surfactant. Both the surfactants on the surface and the interface can stabilize or destabilize the short-wave instabilities, which occur for negligible surface and interfacial tensions.
    Type of Medium: Online Resource
    ISSN: 0022-1120 , 1469-7645
    URL: Article
    DOI: 10.1017/S0022112007008646
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2007
    detail.hit.zdb_id: 1472346-3
    detail.hit.zdb_id: 218334-1
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  • 5
    Online Resource
    Online Resource
    Effects of wall suction/injection on the linear stability of flat Stokes layers
    Cambridge University Press (CUP) ; 2006
    In:  Journal of Fluid Mechanics Vol. 551, No. -1 ( 2006-3-9), p. 303-
    Details
    In: Journal of Fluid Mechanics, Cambridge University Press (CUP), Vol. 551, No. -1 ( 2006-3-9), p. 303-
    Type of Medium: Online Resource
    ISSN: 0022-1120 , 1469-7645
    URL: Article
    DOI: 10.1017/S0022112005008554
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2006
    detail.hit.zdb_id: 1472346-3
    detail.hit.zdb_id: 218334-1
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  • 6
    Online Resource
    Online Resource
    Nesting biology and population dynamics of Jankowski's Bunting Emberiza jankowskii in Western Jilin, China
    Cambridge University Press (CUP) ; 2008
    In:  Bird Conservation International Vol. 18, No. 2 ( 2008-06), p. 153-163
    Details
    In: Bird Conservation International, Cambridge University Press (CUP), Vol. 18, No. 2 ( 2008-06), p. 153-163
    Abstract: Jankowski's Bunting Emberiza jankowskii is endemic to China, Russia and Northern Korea, and was listed as a ‘Vulnerable’ species. The population in Dagang Forestry of western Jilin is one of the small remaining discrete breeding populations in the species' range. Very little information on the nesting biology and population dynamics has previously been published. We studied the nesting biology from 1999 to 2002 and population dynamics of the bunting from 1999 to 2006 (except 2003). A total of 74 nesting attempts were monitored. Jankowski's Bunting breeding season began in late April and usually ended in late July. Both sexes participated in nest-building, feeding young and defending the nest. Mean full clutch size for three years combined was 5.26 ± 0.76 eggs, and ranged from four to seven. Clutch size decreased with nest-initiation date. Mean hatching rate was 41.2%. Overall probability of Mayfield nest success to fledging was low for the three years, averaging 0.218 ± 0.007. The factors leading to low nest success include nest parasitization, nest predation, human activities and nest abandonment. Low survival of Jankowski's Bunting nests may be a factor in declining populations and the slow recovery of populations because of low recruitment at the population and the individual level. The population of Jankowski's Bunting in the Dagang Forestry grassland was small and declined dramatically from 1999 to 2006. The main threat is habitat loss and fragmentation due to agriculture, tree planting and housing following human colonization of the region. The habitat has been reduced in extent by c. 70% since the 1960s. In addition, grazing by domestic livestock dramatically destroyed their preferred vegetation. Furthermore, the restriction to several small, discrete sites makes the bunting inherently vulnerable to catastrophic and stochastic events that can eliminate subpopulations. Jankowski's bunting is one of the most threatened species in China and faces an unpredictable future. Maintaining the structure and general composition of remaining Jankowski's Bunting nesting habitat is important to ensure continued presence of this species in western Jilin and worldwide.
    Type of Medium: Online Resource
    ISSN: 0959-2709 , 1474-0001
    URL: Article
    DOI: 10.1017/S0959270908000154
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2008
    detail.hit.zdb_id: 2037673-X
    SSG: 12
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  • 7
    Online Resource
    Online Resource
    Mechanism of the long-wave inertialess instability of a two-layer film flow
    Cambridge University Press (CUP) ; 2008
    In:  Journal of Fluid Mechanics Vol. 608 ( 2008-08-10), p. 379-391
    Details
    In: Journal of Fluid Mechanics, Cambridge University Press (CUP), Vol. 608 ( 2008-08-10), p. 379-391
    Abstract: This paper provides an intuitive interpretation of the long-wave inertialess instability of a two-layer film flow. The underlying mechanism is elucidated by inspecting the longitudinal perturbation velocity associated with the surface and interfacial deflections. The velocity is expressed by the composition of three parts, related to the shear stress at the free surface, the continuity condition at the interface, and the pressure disturbance induced by gravity. The effect of each velocity component on the evolutions of the surface and the interface is examined in detail. Specifically, the growth of the free surface is caused by the continuity-induced first-order velocity disturbance associated with an additional phase shift between the surface and interfacial waves, while the growth of the interface is due to the pressure-driven flow. The proposed mechanism gives an alternatively reliable prediction of the wave velocity and growth rate.
    Type of Medium: Online Resource
    ISSN: 0022-1120 , 1469-7645
    URL: Article
    DOI: 10.1017/S0022112008002437
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2008
    detail.hit.zdb_id: 1472346-3
    detail.hit.zdb_id: 218334-1
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  • 8
    Online Resource
    Online Resource
    Effect of surfactants on the long-wave stability of oscillatory film flow
    Cambridge University Press (CUP) ; 2006
    In:  Journal of Fluid Mechanics Vol. 562 ( 2006-9), p. 345-
    Details
    In: Journal of Fluid Mechanics, Cambridge University Press (CUP), Vol. 562 ( 2006-9), p. 345-
    Type of Medium: Online Resource
    ISSN: 0022-1120 , 1469-7645
    URL: Article
    DOI: 10.1017/S002211200600139X
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2006
    detail.hit.zdb_id: 1472346-3
    detail.hit.zdb_id: 218334-1
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  • 9
    Online Resource
    Online Resource
    AFLP fingerprinting analysis of genetic polymorphism of 12 indigenous chicken breeds
    Cambridge University Press (CUP) ; 2007
    In:  Chinese Journal of Agricultural Biotechnology Vol. 4, No. 1 ( 2007-04), p. 33-38
    Details
    In: Chinese Journal of Agricultural Biotechnology, Cambridge University Press (CUP), Vol. 4, No. 1 ( 2007-04), p. 33-38
    Abstract: A total of six amplified fragment length polymorphism (AFLP) primer combinations were used to detect genetic variation of pooled DNA in a sample of 12 chicken breeds indigenous to China, and AFLP DNA fingerprinting of each chicken breed was constructed. Polymorphic bands, specific bands and genetic similarity coefficients of 12 chicken breeds were calculated from AFLP data. A total of 279 polymorphic bands were generated by the six primer combinations, giving, on average, 46.5 polymorphic markers detected per primer combination. Nine specific bands were produced in the pooled DNA of Jiuyuan black and Dongxiang black chickens. However, one specific band was produced in the pooled DNA of Wenchang and Xingyi bantam chickens. An unweighted-pair-group method using average linkages (UPGMA) cluster analysis revealed that the 12 chicken breeds could be divided into three groups. Genetic similarity coefficients and the UPGMA tree of the 12 chicken breeds were consistent with their breeding history as well as their geographical distribution. Based on AFLP DNA fingerprinting, genetic diversity, genetic relationship and identification of chicken breeds can be analysed.
    Type of Medium: Online Resource
    ISSN: 1479-2362 , 1479-2370
    URL: Article
    DOI: 10.1017/S1479236207001234
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2007
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  • 10
    Online Resource
    Online Resource
    Recognition of features of parts subjected to motion using ARTMAP incorporated in a flexible vibratory bowl feeder system
    Cambridge University Press (CUP) ; 2006
    In:  Artificial Intelligence for Engineering Design, Analysis and Manufacturing Vol. 20, No. 1 ( 2006-02), p. 19-34
    Details
    In: Artificial Intelligence for Engineering Design, Analysis and Manufacturing, Cambridge University Press (CUP), Vol. 20, No. 1 ( 2006-02), p. 19-34
    Abstract: The recognition and identification of parts are important processes in modern manufacturing systems. Although machine vision systems have played an important role in these tasks, there are still challenges in performing these tasks in which parts may be in motion and subjected to noise. Using a flexible vibratory bowl feeder system as a test bed to simulate motion of parts subjected to noise, scanned signatures of part features are acquired using fiber optic sensors and a data acquisition system. Because neural networks have been shown to exhibit good pattern recognition capability, ARTMAP, a neural network that learns patterns under supervision, was incorporated into the feeder system. The pattern recognition capability of the feeder system is dependent on a set of parameters that characterized ARTMAP, the sampling rate of the data acquisition system, and the mean speed of the vibrating parts. The parameters that characterized ARTMAP are the size of an input vector, the vigilance, threshold value of the nonlinear noise suppression function, and the learning rate. Through extensive training and testing of the ARTMAP within the feeder system, it was shown that high success rates of recognition of parts features in motion under noisy conditions can be obtained provided these parameters of ARTMAP are appropriately selected.
    Type of Medium: Online Resource
    ISSN: 0890-0604 , 1469-1760
    URL: Article
    DOI: 10.1017/S0890060406060021
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2006
    detail.hit.zdb_id: 2025375-8
    SSG: 9,11
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