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  • 2005-2009  (1)
  • 2000-2004  (3)
  • 1975-1979  (6)
  • 1960-1964  (5)
  • 1
    Book
    Book
    New York : Micropaleontology Press
    Type of Medium: Book
    Pages: VI, 198 S , Ill., graph. Darst
    Series Statement: Micropaleontology 46, Suppl. 1
    Language: English
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 7 (1960), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Three strains of Hypotrichomonas acosta were isolated in axenic culture. Attempts to develop a defined medium directly from a defined medium suitable for Tetrahymena pyriformis were unsuccessful. Development of partly defined media by substitution for undefined materials in Diamond's medium were more successful. Horse serum was replaced by 1 mg. % TEM-4T (a diacetyl tartaric acid ester of monoglycerides from tallow) and 0.5 mg.% cholesterol. Yeast extract was replaced by a mixture of ribonucleotides. Inclusion of several additional components permitted reducing the Trypticase concentration from 2% to 0.25%.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 7 (1960), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Hypotrichomonas gen nov., with a feeble undulating membrane but lacking a costa, is placed in the family Monocercomonadidae Kirby of the order Trichomonadida Kirby. H. acosta (Moskowitz) from squamate reptiles is designated as the type species. It has 3 unequal anterior flagella. The proximal part of its recurrent flagellum is attached to the surface of the body along an accessory filament. A low membrane with typically a few undulations extends between the accessory filament and the body surface. The membrane, which varies greatly in length in relation to the length of the body among strains of the species, is not supported by a costa. The recurrent flagellum continues as a free posterior flagellum beyond the end of the undulating membrane and terminates in an acroneme. The capitulum of the axostyle, spatulate or scoop-shaped, is closely applied to the anteriorly located nucleus. The capitulum extends anteriorly into a crescent-shaped pelta. The relatively stout trunk of the axostyle is straight or twisted. It projects for some distance from the posterior body surface, and the terminal segment of the projecting part tapers gradually to a point. The parabasal apparatus consists of a rod- or shaped body and of a filament or filaments.In division, the full complement of flagella in each of the mastigonts is restored relatively late during division but before cytokinesis. One of the daughter individuals retains the parental undulating membrane; the other develops a new organelle. The development of the new membrane is first marked by the appearance of an acroneme, presumably recurrent flagellum, early in division. The proximal part of this flagellum becomes progressively attached to the body surface. The process of attachment is paralleled by differentiation of the accessory filament and is followed by the appearance of the undulating membrane. The parental axostyle is resorbed during division, and each daughter individual develops this organelle de wove. In the very early stages of division the parabasal consisted of two arms and in all more advanced stages of a single rod-shaped body applied to a single filament.The significance of Hypotrichonionas in the evolution of trichomonads is discussed. Its morphology and morphogenesis suggest that this genus may represent an important step in the evolution of Trichomonadidae from Monocercomonadidae.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 23 (1976), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. It was demonstrated with the aid of light and electron microscope studies that Sorites marginalis (Lamarck) harbors zooxanthellae. The hosts were scraped from Thalassia testudinum Konig growing in Key Largo Sound (Florida, U.S.A.) and immediately preserved in appropriate fixatives. Zooxanthellae were distributed unevenly throughout all the chamberlets; only a few symbiotes were found in the embryonic chambers and the inner or outer chambers, but the intermediate chambers were packed with symbiotes. The outer chambers contained many food vacuoles in addition to symbiotes. Some zooxanthellae might have been in the process of degeneration or digestion. The symbiotes were found to have a typical dinoflagellate nucleus, a single large lobate cortical chloroplast with one stalked pyrenoid, an accumulation body, and many starch granules. The nonmotile stage of the zooxanthella was similar, but perhaps not identical, to Symbiodinium microadriaticum Freudenthal from various hosts.The foraminiferan host is heterokaryotic with hundreds of generative (small) nuclei and scores of vegetative (large) nuclei. Most of the generative nuclei were found in the embryonic apparatus and the inner chambers. Most of the vegetative nuclei were found in the inner and outer chambers.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 23 (1976), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The life cycles of 3 strains of Allogromia laticollaris, a monothalamous foraminiferan, have been studied. Each of the strains had a different, nonclassical, and basically apogamic, life cycle. The Cold Spring Harbor (CSH) strain regularly alternated between 2 agamontic forms: agamont I (uninucleate and diploid) and agamont II (multinucleate and diploid). The complete life cycle took 26 days. Sexual reproduction was rare (0.01%) and autogamous. Small numbers of organisms also underwent budding, binary fission, and cytotomy. The life cycles of the Towd Point (TPA) and Sippewissett (SIP) strains were comparatively abbreviated. Agamont II dominated their typical life cycles, which were completed in 16-18 days. The life cycle of SIP was basically a continuous cycling of the agamont II phase. Approximately 75% of the schizozoites of the TPA strain developed into agamont II. The other 25% alternated between agamont II and agamont I phase.In the CSH strain schizozoites with ∼ 8 (range 5-15) nuclei characterized newly formed agamonts II. More nuclei (∼ 25) were found in the other 2 strains. The nuclei in young agamonts II underwent rapid morphologic changes leading to a “mushroom-like” chromosome appearance and extensive RNA synthesis. Nucleolar material accumulated at the nuclear periphery and eventually was discharged to the cytoplasm. Karyokinesis took place without the breakdown of the nuclear membrane.The single nucleus of young agamont I forms was proportionally quite large. The S1 phase occurred quite early (2-5 days) in this part of the life cycle. RNA in the CSH strain formed a compact, subcortical, coarsely granular ring, while in the TPA it was cortical and differentiated into finely granular matrix with randomly distributed coarse granules. During the G2 phase the nucleus became further enlarged and eventually amoeba-form. Intermediate stages in nuclear breakdown were not found.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Several trichomonads from poikilotherms (Hypotrichomonas acosta, Monocercomonas colubrorum and M. sp., Trichomonas batrachorum, and Tritrichomonas augusta) grew in a nearly-defined medium to a level of 10–40% that permitted with media containing casein digests; T. augusta (4 strains) grew best. The defined medium contained TEM-4T (a semisynthetic fat based on beef-tallow fatty acids) and ascorbyl “palmitate” as sources of fatty acids. Besides ascorbyl palmitate, thiomalic and ascorbic acids, and tocopherol served as reducers and antiöxidants. High concentrations of nucleotides (adenylic, cytidylic, guanylic, and uridylic acids) seemed necessary. Indigo carmine was a useful redox indicator; the flagellates never grew in the oxidized zone. Extra trace elements and amino acids or rich natural media permitted better growth at about 34.5°C—the upper temperature limit for these trichomonads, raising the issue of a biochemical basis of a thermal barrier to the invasion of warmblooded hosts. Except for the limited carbohydrate in the nucleotides, carbohydrate could be omitted, the main substrates being a combination of glycerol, acetate, and gylcero-phosphate, posing the question of the identity of the anaerobic, presumably non-glycolytic, energy-yielding pathway.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 10 (1963), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Two new dinoflagellates, isolated from Long Island Sound and maintained axenically, are described. Gyrodinium instriatum n. sp. changes shape and size as cultures age. Glenodinium halli n. sp. is a delicately-armored form whose plate structure differs slightly from the present diagnosis of the genus, but not sufficiently like any other genus in morphology or life history for inclusion elsewhere. In G. halli the size of the motile form and predominance of motile or vegetative stages are nutrient dependent. Both dinoflagellates illustrate the need for morphological descriptions based upon continuous culture methods in defined media.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 24 (1977), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 10 (1963), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A polymorphic allogromiid (our strain NF) was isolated in monoxenic culture but attempts at its axenic culture failed. Growth of monoxenic cultures was stimulated by various metals and vitamins. The morphology and life cycle of this allogromiid with a prominent collar have been studied in detail. It varied in form from ovoid to elongate bioral “Shepheardella-like” forms and to irregular polyoral organisms. The organisms ranged in length from 56–385 μ (118 μ± 50.54) and in width from 35–385 μ (99 μ± 39.34). The number of nuclei per organism averaged 8.9 ± 6.3 (range 1–40). Reproduction of “Shepheardella-like” forms was by binary fission. Three types of budding have been observed and, rarely, schizogony. No evidence for sexual reproduction has thus far been seen.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Inc
    The @journal of eukaryotic microbiology 52 (2005), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Various methodological approaches are being used to characterize Symbiodinium spp isolated from Marginopora vertebralis and Amphisorus hemprichii and Amphisorus sp. from the Indo-pacific. While most of the cells in culture are non-motile vegetative cells, dinospores occur in batch cultures. We find consistant variance among the isolates in the duration and time of appearance of motile forms. Nitrate (0.146 mM) supports higher populations of the isolates than NH4 (0.146 mM). Higher concentrations of NH4 inhibit growth. Although Fensome et al. (1993) characterized the Order Suessiales (and Family Symbiodiniaceae) as gymnnodiniphycideans in which the amphiesmal vesicals are arranged in 7–10 longitudinal series we were unable to detect any plates on the surfaces of our isolates in the SEM. We were unsuccessful in revealing plates on non-motile forms by freeze fracture but they were present after treatment with various chemical agents (e.g. H2O2, HOCl−) and enzymes (cellulase, chitinase). They are not polygonal or arranged in the patterns anticipated for the Symbiodiniaceae. Polyclonal antibody studies of the Symbiodinium-like isolates are in agreement with molecular systematic studies in the sense that there is a common relation. The foram endosymbiotic dinoflagellates share some common surface antigens with each other and with the few invertebrate isolates we have tested. Studies of the foraminiferal symbionts' plastids revealed considerable morphological diversity. We isolated a diversity of endosymbionts from the mantle of Tridacna maxima from the Red Sea and the Marshall Islands. These included Symbiodinium, Amphidinium, Tetraselmis, and an unidentified chlorophyte.
    Type of Medium: Electronic Resource
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