In:
Journal of AOAC INTERNATIONAL, Oxford University Press (OUP), Vol. 94, No. 4 ( 2011-07-01), p. 1100-1105
Abstract:
An HPLC/MS/MS method for the determination of arbidol in human plasma was developed. Arbidol and internal standard (loratadine) were extracted from alkaline plasma with tert-butyl methyl ether and analyzed on a Zorbax SB C18 column (30 × 2.1 mm id, 3.5 µm particle size). The detection was by monitoring arbidol at m/z 479.1 → 434.1 and the internal standard at m/z 383.2 → 337.2. The method was validated according to U.S. Food and Drug Administration guidelines. The calibration curve was linear over the range of 0.5–500 ng/mL using a 100 µL sample volume. The intraday and interday precisions were less than 6.5%, and acceptable values were obtained for accuracy, recovery, and sensitivity. The developed method was selective, simple, sensitive, and easily applicable.
Type of Medium:
Online Resource
ISSN:
1060-3271
,
1944-7922
DOI:
10.1093/jaoac/94.4.1100
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2011
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