In:
Journal of Integrative Plant Biology, Wiley, Vol. 56, No. 3 ( 2014-03), p. 315-332
Abstract:
In halophytic plants, the high‐affinity potassium transporter HKT gene family can selectively uptake K + in the presence of toxic concentrations of Na + . This has so far not been well examined in glycophytic crops. Here, we report the characterization of SbHKT1;4 , a member of the HKT gene family from Sorghum bicolor . Upon Na + stress, SbHKT1;4 expression was more strongly upregulated in salt‐tolerant sorghum accession, correlating with a better balanced Na + /K + ratio and enhanced plant growth. Heterogeneous expression analyses in mutants of Saccharomyces cerevisiae and Arabidopsis thaliana indicated that overexpressing SbHKT1;4 resulted in hypersensitivity to Na + stress, and such hypersensitivity could be alleviated with the supply of elevated levels of K + , implicating that SbHKT1;4 may mediate K + uptake in the presence of excessive Na + . Further electrophysiological evidence demonstrated that SbHKT1;4 could transport Na + and K + when expressed in Xenopus laevis oocytes. The relevance of the finding that SbHKT1;4 functions to maintain optimal Na + /K + balance under Na + stress to the breeding of salt‐tolerant glycophytic crops is discussed.
Type of Medium:
Online Resource
ISSN:
1672-9072
,
1744-7909
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2130095-1
SSG:
12
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