In:
European Journal of Haematology, Wiley, Vol. 89, No. 4 ( 2012-10), p. 302-310
Abstract:
Spindle‐shaped diffuse large B‐cell lymphoma (Sp‐ DLBCL ) has been recognized as a rare morphologic variant of DLBCL . However, the biological processes that contribute to the specific features of Sp‐ DLBCL remain poorly understood. In this study, a combined immunophenotypic and genetic analysis was performed in 10 Sp‐ DLBCL . First, we investigated several unique markers for anaplasia ( CD 30, ALK , CD 68, and EBER ‐ ISH ), mesenchyma ( SMA , desmin, and vimentin), and B‐cell differentiation ( CD 10, BCL 6 , and MUM 1). We also performed conventional cytogenetic and fluorescence in situ hybridization studies to look for common chromosomal break points ( BCL 2 , BCL 6 , and MYC ). We found that most Sp‐ DLBCL s were germinal center B cell‐like and that none had any other specific phenotypes or any karyotypic abnormalities. Instead, T cells, CD 68‐positive macrophages and SMA ‐positive myofibroblasts were significantly increased in Sp‐ DLBCL when compared with conventional GCB origin DLBCL cases ( n = 10) ( P = 0.012, P 〈 0.001, and P 〈 0.0001, respectively). To further characterize Sp‐ DLBCL , we next compared the expression of fibroblast growth factor 2 ( FGF 2) and transforming growth factor‐β1 ( TGF β1) between the two types of DLBCL . Finally, we confirmed that the number of FGF 2‐ and TGF β1‐positive stromal cells was markedly increased in Sp‐ DLBCL and that the difference between these and conventional GCB origin DLBCL s was significant ( P 〈 0.0001 and P = 0.0017, respectively). Thus, T‐cell/myofibrohistio‐rich stromal alterations in Sp‐ DLBCL , especially those mediated by TGF β1 and FGF 2, may play a role in the transition of lymphoma cells into those with spindle‐shaped features.
Type of Medium:
Online Resource
ISSN:
0902-4441
,
1600-0609
DOI:
10.1111/ejh.2012.89.issue-4
DOI:
10.1111/j.1600-0609.2012.01826.x
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
2027114-1
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