In:
PROTEOMICS, Wiley, Vol. 10, No. 20 ( 2010-10), p. 3723-3731
Abstract:
microRNA‐122a (miR‐122a) is a liver‐specific miRNA that is frequently downregulated in hepatocellular carcinoma (HCC). The exact functional role of miR‐122a and its target in HCC remain largely unknown. We developed a lentiviral vector for the expression of pre‐miR‐122a (Lenti‐miR‐122a). Lenti‐miR‐122a inhibited HCC cell growth and induced apoptosis in vitro . We employed proteomic profiling to identify the target proteins of miR‐122a. In total, ten proteins with differential expression in HCC cells infected with Lenti‐miR‐122a were identified. Amongst them, downregulation of peroxiredoxin 2 (PRDXII) by miR‐122a was validated by Western blotting. Using bioinformatics analysis, predictable target sites of miR‐122a were identified in the 5′‐UTR of PRDXII mRNA. Luciferase reporter assay confirmed the regulation of miR‐122a on 5′‐UTR of PRDXII. In conclusion, PRDXII was identified to be the new target of miR‐122a.
Type of Medium:
Online Resource
ISSN:
1615-9853
,
1615-9861
DOI:
10.1002/pmic.201000050
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
2037674-1
SSG:
12
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