In:
Cell Biochemistry and Function, Wiley, Vol. 29, No. 4 ( 2011-06), p. 294-302
Abstract:
Dendritic cells (DCs) are professional antigen‐presenting cells that play an important role in anti‐tumour immunity. Endothelial‐like differentiation of DCs is an interesting phenomenon. The specific role of vascular endothelial growth factor‐A (VEGF‐A) on the differentiation of immature DCs (iDCs) and mature DCs (mDCs) is worth further research. Here, we show that VEGF‐A can induce iDCs to differentiate into endothelial‐like cells (ELCs). But it has no obvious influence on mDCs. In the process of endothelial‐like differentiation of iDCs, a sustained activation of extracellular signal‐regulated kinase (ERK1/2) and cAMP response element binding protein (CREB) was detected. VEGF‐A induced the activation of ERK1/2, and led to the nuclear translocation of phosphorylation ERK1/2. Incubation of iDCs with the ERK1/2 upstream kinase MEK1/2 inhibitor PD98059, blocked the phosphorylation of ERK1/2 and CREB as well as the endothelial‐like differentiation of iDCs. These data suggest that VEGF‐A induces endothelial‐like differentiation of iDCs not mDCs through ERK1/2 signalling pathway. Copyright © 2011 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
0263-6484
,
1099-0844
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
1496553-7
SSG:
12
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