In:
Molecular Ecology Resources, Wiley, Vol. 13, No. 1 ( 2013-01), p. 57-65
Abstract:
The island of M adagascar is a key hot spot for the genus E uphorbia , with at least 170 native species, almost all endemic. Threatened by habitat loss and illegal collection of wild plants, nearly all M alagasy E uphorbia are listed in CITES A ppendices I and II . The absence of a reliable taxonomic revision makes it particularly difficult to identify these plants, even when fertile, and thereby compromises the application of CITES regulations. DNA barcoding, which can facilitate species‐level identification irrespective of developmental stage and the presence of flowers or fruits, may be a promising tool for monitoring and controlling trade involving threatened species. In this study, we test the potential value of barcoding on 41 E uphorbia species representative of the genus in M adagascar, using the two widely adopted core barcode markers ( mat K and rbc L ), along with two additional DNA regions, nuclear internal transcribed spacer ( ITS ) and the chloroplastic intergenic spacer psbA‐trn H . For each marker and for selected marker combinations, inter‐ and intraspecific distance estimates and species discrimination rates are calculated. Results using just the ‘official’ barcoding markers yield overlapping inter‐ and intraspecific ranges and species discrimination rates below 60%. When ITS is used, whether alone or in combination with the core markers, species discrimination increases to nearly 100%, whereas the addition of psb A ‐trn H produces less satisfactory results. This study, the first ever to test barcoding on the large, commercially important genus E uphorbia shows that this method could be developed into a powerful identification tool and thereby contribute to more effective application of CITES regulations.
Type of Medium:
Online Resource
ISSN:
1755-098X
,
1755-0998
DOI:
10.1111/men.2012.13.issue-1
DOI:
10.1111/1755-0998.12028
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2406833-0
SSG:
12
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