In:
IUBMB Life, Wiley, Vol. 66, No. 3 ( 2014-03), p. 220-227
Abstract:
As a group of heterogeneous multipotent cells, mesenchymal stem cells (MSCs) have potential in treatment of a variety of clinical diseases. However, the low survival of the transplanted MSCs reduced their therapeutic effects. In this study, we revealed that rno‐miR‐203 suppressed activity and colony formation and enhanced apoptosis of the rat bone marrow‐derived MSCs (BM‐MSCs). Using bioinformatics analysis, we found a potential miR‐203 binding site within rat phosphatidylinositol 3‐kinase (PI3K) 3′UTR, and fluorescent reporter experiments validated the direct and negative regulation of PI3K expression by miR‐203 through this site. Ectopic expression of PI3K rescued BM‐MSCs from depressed activity induced by miR‐203, and suppression of PI3K attenuated the increased BM‐MSCs activity by miR‐203 inhibitor treatment. Moreover, miR‐203 blocking partly protected BM‐MSCs from impairment caused by low nutrition. We conclude that inhibition of endogenous miR‐203 elevated PI3K expression, which may strengthen PI3K/Akt pathway and promote BM‐MSCs activity and survival. © 2014 IUBMB Life, 66(3):220–227, 2014
Type of Medium:
Online Resource
ISSN:
1521-6543
,
1521-6551
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2009952-6
detail.hit.zdb_id:
1492141-8
SSG:
12
Permalink