In:
Otolaryngology–Head and Neck Surgery, Wiley, Vol. 147, No. 6 ( 2012-12), p. 1020-1026
Abstract:
The purpose of the present study was to investigate the effect of nucleotide‐binding oligomerization domain 1 (NOD1), an innate immune sensor, on allergic inflammation and induction of regulatory T cells in a mouse model of allergic rhinitis. We also aimed to explore whether there were differences in the effect of NOD1 ligand according to the timing of administration. Study Design An in vivo study using an animal model. Setting Catholic Research Institutes of Medical Science. Subjects and Methods Forty BALB/c mice were divided into 4 groups: control, OVA, pre‐NOD1, and post‐NOD1. Ovalbumin (OVA) was used for sensitization and challenge. The pre‐NOD1 group received NOD1 ligand intranasally before sensitization, whereas the post‐NOD1 group received it after sensitization. The effects of allergic inflammation and regulatory T cells were compared among the groups. Results In the post‐NOD1 group, serum OVA‐specific IgE, eosinophil counts, interleukin (IL)–13 levels, and GATA‐3 mRNA expression were significantly increased and Foxp3 + mRNA expression and CD4 + Foxp3 + T cells were decreased compared with the OVA group. In the pre‐NOD1 group, Foxp3 mRNA expression and CD4 + Foxp3 + T cells were significantly decreased compared with the OVA group. Although not significant, the pre‐NOD1 group showed increases in serum OVA‐specific IgE, eosinophil counts, IL‐13 levels, and GATA‐3 mRNA expression compared with the OVA group. Conclusion The innate immune response through NOD1 enhances allergen‐specific Th2 response and suppresses induction of regulatory T cells in a mouse model of allergic rhinitis, and the effects are different depending on the timing of exposure to NOD1 ligand.
Type of Medium:
Online Resource
ISSN:
0194-5998
,
1097-6817
DOI:
10.1177/0194599812461999
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
2008453-5
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