In:
Journal of Biomedical Science, Springer Science and Business Media LLC, Vol. 18, No. 1 ( 2011-12)
Abstract:
The extracellular calcium-sensing receptor (CaSR) belongs to family C of the G protein coupled receptors. Whether the CaSR is expressed in the pulmonary artery (PA) is unknown. Methods The expression and distribution of CaSR were detected by RT-PCR, Western blotting and immunofluorescence. PA tension was detected by the pulmonary arterial ring technique, and the intracellular calcium concentration ([Ca 2+ ] i ) was detected by a laser-scanning confocal microscope. Results The expressions of CaSR mRNA and protein were found in both rat pulmonary artery smooth muscle cells (PASMCs) and PAs. Increased levels of [Ca 2+ ] o (extracellular calcium concentration) or Gd 3+ (an agonist of CaSR) induced an increase of [Ca 2+ ] i and PAs constriction in a concentration-dependent manner . In addition, the above-mentioned effects of Ca 2+ and Gd 3+ were inhibited by U73122 (specific inhibitor of PLC), 2-APB (specific antagonist of IP 3 receptor), and thapsigargin (blocker of sarcoplasmic reticulum calcium ATPase). Conclusions CaSR is expressed in rat PASMCs, and is involved in regulation of PA tension by increasing [Ca 2+ ] i through G-PLC-IP 3 pathway.
Type of Medium:
Online Resource
ISSN:
1423-0127
DOI:
10.1186/1423-0127-18-16
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2011
detail.hit.zdb_id:
1482918-6
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