In:
Journal of Pharmacy and Pharmacology, Oxford University Press (OUP), Vol. 63, No. 9 ( 2011-08-09), p. 1169-1174
Abstract:
Previously, the flavonoid (±)-catechin was shown to exert potent neuroprotective action in the mouse 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson's disease model. The purpose of this study was to investigate whether the different enantiomers of catechin ((+)-catechin, (−)-catechin and (±)-catechin, a 50 : 50 mixture of (+)-catechin and (−)-catechin) could protect SH-SY5Y cells against 1-methyl-4-phenylpyridinium ion (MPP+) toxicity by decreasing the generation of oxygen free radicals. The inhibitive effect of (±)-catechin on JNK/c-Jun activation was investigated. Methods The effects of (+)-catechin, (−)-catechin or (±)-catechin in protecting against MPP+ toxicity were evaluated and compared in SH-SY5Y cells by testing the release of lactate dehydrogenase. The generation of reactive oxygen species (ROS) was measured by immunochemistry and the phosphorylation level of JNK/c-Jun was determined by Western blotting. Key findings In SH-SY5Y cells, (+)-catechin, (−)-catechin or (±)-catechin reduced apoptosis induced by MPP+ and decreased ROS generation caused by MPP+. Different enantiomers of catechin showed protective effects at similar potency. Moreover (±)-catechin decreased JNK/c-Jun phosphorylation which was increased by MPP+. Conclusions Catechin and its two enantiomers could protect SH-SY5Y cells against MPP+ cytotoxicity at a similar potency. Antioxidative stress and inhibition of the JNK/c-Jun signalling pathway might have been involved in the neuroprotective mechanisms of catechin against MPP+ cytotoxicity in SH-SY5Y cells.
Type of Medium:
Online Resource
ISSN:
0022-3573
,
2042-7158
DOI:
10.1111/j.2042-7158.2011.01293.x
Language:
English
Publisher:
Oxford University Press (OUP)
Publication Date:
2011
detail.hit.zdb_id:
2041988-0
detail.hit.zdb_id:
2050532-2
SSG:
15,3
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