In:
Molecular Systems Biology, EMBO, Vol. 9, No. 1 ( 2013-01)
Abstract:
A new genome‐scale metabolic reconstruction of M. pneumonia is used in combination with external metabolite measurement and protein abundance measurements to quantitatively explore the energy metabolism of this genome‐reduce human pathogen. image We established a detailed biomass composition for M. pneumoniae , thus allowing for growth simulations. Using our metabolic model, we corrected the metabolic network topology and the functional annotation of key metabolic enzymes. M. pneumoniae , unlike other laboratory‐grown bacteria, uses a high fraction of energy (up to 89%) for cellular maintenance and not for growth. Simulating different growth conditions as well as single and double mutant phenotypes, we analyzed pathway connectivity and the impact of gene deletions on the growth performance of M. pneumoniae , highlighting the limited adaptive capabilities of this minimal model organism.
Type of Medium:
Online Resource
ISSN:
1744-4292
,
1744-4292
Language:
English
Publisher:
EMBO
Publication Date:
2013
detail.hit.zdb_id:
2193510-5
SSG:
12
Permalink