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  • The American Society for Microbiology (ASM)  (65)
  • Wiley-Blackwell  (22)
  • 2010-2014  (81)
  • 1995-1999  (6)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 41 (1995), S. 214-222 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Nucleation and growth of bubbles in porous media are important problems encountered in processes such as pressure depletion and boiling. Experiments and numerical simulations are studied in micromodel geometries to understand their basic aspects. Experiments of bubble growth by pressure depletion are carried out in 2-D etched-glass micromodels and in Hele-Shaw cells. Nucleation of bubbles and subsequent growth of gas clusters are visualized. Contrary to the bulk or Hele-Shaw cells, gas clusters in the micromodel have irregular and ramified shapes and share many of the features of an external invasion process (such as percolation during drainage). A pore network numerical model developed simulates the growth of multiple gas clusters under various conditions. It is based on the solution of the convection-diffusion equation and accounts for capillary and viscous forces, which play an important role in determining growth patterns. Numerical simulation agrees well with the experimental results.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 41 (1995), S. 1534-1542 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In recent years, the circulating fluidized bed as a reactor has experienced increasing application in industry. A circulating fluidized bed (CFB) has some unique features as a chemical reactor. The performance of the CEB reactor was investigated in a O.25-m-dia. Riser system with ozone decomposition in the reactor. Both axial and radial profiles of ozone concentration are presented, as well as overall conversions under various reaction conditions. The effects of the operating conditions on the performance of the CFB reactor were examined. The experimental results show that the performance of a circulating fluidized bed as a reactor is much nearer to that of a well-mixed system than that of a plug-flow system. The effectiveness factor of the reactor seems to decrease with increase of solids holdup in the reactor.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1075-4261
    Keywords: cholelithiasis ; gallstone ; bile salts ; periodic precipitation ; fractal precipitation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: This is the first observation that both chaotic and periodic patterns are formed in metal ions-deoxycholate-gel systems. It is an in vitro model for approximating the conditions present during gallstone formation. The experimental results suggest that a nonlinear scientific concept such as the “butterfly effect” should be considered in understanding gallstone formation. This effect suggests that a butterfly flapping its wings in Beijing today may lead to a thunderstorm in New York months later. Applying this concept to biology, minor changes in the local chemical environment within biological systems may lead to large variations in the structure and morphology of gallstone through changes in the behavior of biological mineralization process. © 1997 John Wiley & Sons, Inc. Biospect 3: 195-205, 1997
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1075-4261
    Keywords: black pigment gallstone ; FTIR ; EPR ; nonlinear phenomena ; vibrational mode ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Further spectroscopic studies of gallstones are reviewed with an emphasis on the formation of black pigment gallstones. This type of gallstone appears mainly in Western countries, with only 3% of the cholelithiasis patients in China having black gallstones. Fourier transform infrared absorption and electron paramagnetic resonance are used as spectroscopic probes of gallstones and their metal bilirubinate components. Nonlinear phenomena in gallstone formation were investigated through the appearance of ring structure in gallstones and fractal patterns in the formation in the precipitates of bile salt systems. Although a complete understanding of gallstone formation has not yet been achieved, interesting progress toward this goal has been made recently. © 1997 John Wiley & Sons, Inc. Biospectroscopy 3: 381-391, 1997
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 2131-2152 
    ISSN: 0029-5981
    Keywords: discontinuous Galerkin finite element method ; predictor-multicorrector ; adaptive time integration ; structural dynamics ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper studies a time-discontinuous Galerkin finite element method for structural dynamic problems, by which both displacements and velocities are approximated as piecewise linear functions in the time domain and may be discontinuous at the discrete time levels. A new iterative solution algorithm which involves only one factorization for each fixed time step size and a few iterations at each step is presented for solving the resulted system of coupled equations. By using the jumps of the displacements and the velocities in the total energy norm as error indicators, an adaptive time-stepping procedure for selecting the proper time step size is described. Numerical examples including both single-DOF and multi-DOF problems are used to illustrate the performance of these algorithms. Comparisons with the exact results and/or the results by the Newmark integration scheme are given. It is shown that the time-discontinuous Galerkin finite element method discussed in this study possesses good accuracy (third order) and stability properties, its numerical implementation is not difficult, and the higher computational cost needed in each time step is compensated by use of a larger time step size.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 39 (1996), S. 619-633 
    ISSN: 0029-5981
    Keywords: gradient plasticity ; strain localization ; mixed strain element method ; large strain ; consistent algorithm ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A finite element method for gradient elasto-plastic continuum in which the yield strength of strain hardening/softening materials not only depends on the effective plastic strain but also on its Laplacian is presented. The consistent integration algorithm to update the stress and the internal state variable at integration points and the consistent compliance matrix for the gradient plasticity are formulated in the non-local sense.The methodology to derive the finite element formulation for the gradient plasticity at large strains presented in this paper is applicable to general finite element analysis; the formulation in the context of the two-dimensional four-noded mixed finite element with one integration point and mean von Mises yield criterion is particularly derived.Numerical examples are tested to demonstrate the capability and performance of the present finite element method at large strain in solving for the strain localization problem.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Publication Date: 2012-12-29
    Description: Infectious bursal disease (IBD) is an acute, highly contagious, and immunosuppressive avian disease caused by IBD virus (IBDV). Although IBDV-induced immunosuppression has been well established, the underlying exact molecular mechanism for such induction is not very clear. We report here the identification of IBDV VP4 as an interferon suppressor by interaction with the glucocorticoid-induced leucine zipper (GILZ) in host cells. We found that VP4 suppressed the expression of type I interferon in HEK293T cells after tumor necrosis factor alpha (TNF-α) treatment or Sendai virus (SeV) infection and in DF-1 cells after poly(I·C) stimulation. In addition, the VP4-induced suppression of type I interferon could be completely abolished by knockdown of GILZ by small interfering RNA (siRNA). Furthermore, knockdown of GILZ significantly inhibited IBDV growth in host cells, and this inhibition could be markedly mitigated by anti-alpha/beta interferon antibodies in the cell cultures ( P 〈 0.001). Thus, VP4-induced suppression of type I interferon is mediated by interaction with GILZ, a protein that appears to inhibit cell responses to viral infection.
    Print ISSN: 0022-538X
    Electronic ISSN: 1098-5514
    Topics: Medicine
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  • 8
    Publication Date: 2011-08-23
    Description: Producing optimal grain yields while reducing adverse environmental impacts of over-fertilization is essential in intensive, but sustainable, farming systems. We investigated the effects of long-term (1982–2005) application of chemical nitrogen (N), N + chemical phosphorus (P) and N + P + chemical potassium (K) on grain yield, nitrogen recovery efficiency (NRE) and N losses in two rice–wheat sites in subtropical China where pig manure was applied (Suining and Wuchang). Four (Suining) or five (Wuchang) treatments were examined: no-fertilizer, chemical N plus manure (NM), chemical NP plus manure (NPM), chemical NPK plus manure (NPKM) or chemical NPK plus 1.6 times manure (NPKhM, Wuchang only). Fertilizers resulted in 1.5–2.5 times higher grain yields than no-fertilizer, which led to a NRE in the range from 21.0 to 58.3%. Grain yields of rice and wheat were significantly increased by 22.6–25.9 and 34.4–37.5%, respectively, under NPM and NPKM (similar to each other) compared to NM at Suining. Yields were similar for NM, NPK, NPKM and NPKhM at Wuchang. The N accumulation and NRE among fertilizers were in the order NM 〈 NPM = NPKM at the low amount of manure-applied site (Suining), but NM = NPM = NPKM at the high amount of manure-applied site (Wuchang). The ratio of N losses to total N input was 21.4–49.1% at the studied sites. Soil total N accumulated at a rate of 0.01–0.04 g/kg/yr during 1982–2005 with fertilizers and decreased or was constant in soil without fertilizer. Application of chemical P and K fertilizers could be reduced or eliminated after long-term manure application at these two sites, while maintaining optimal grain yields and enhancing soil N accumulation.
    Print ISSN: 0266-0032
    Electronic ISSN: 1475-2743
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley-Blackwell
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  • 9
    Publication Date: 2013-11-20
    Description: The development of a safe and efficient dengue vaccine represents a global challenge in public health. Chimeric dengue viruses (DENV) based on an attenuated flavivirus have been well developed as vaccine candidates by using reverse genetics. In this study, based on the full-length infectious cDNA clone of the well-known Japanese encephalitis virus live vaccine strain SA14-14-2 as a backbone, a novel chimeric dengue virus (named ChinDENV) was rationally designed and constructed by replacement with the premembrane and envelope genes of dengue 2 virus. The recovered chimeric virus showed growth and plaque properties similar to those of the parental DENV in mammalian and mosquito cells. ChinDENV was highly attenuated in mice, and no viremia was induced in rhesus monkeys upon subcutaneous inoculation. ChinDENV retained its genetic stability and attenuation phenotype after serial 15 passages in cultured cells. A single immunization with various doses of ChinDENV elicited strong neutralizing antibodies in a dose-dependent manner. When vaccinated monkeys were challenged with wild-type DENV, all animals except one that received the lower dose were protected against the development of viremia. Furthermore, immunization with ChinDENV conferred efficient cross protection against lethal JEV challenge in mice in association with robust cellular immunity induced by the replicating nonstructural proteins. Taken together, the results of this preclinical study well demonstrate the great potential of ChinDENV for further development as a dengue vaccine candidate, and this kind of chimeric flavivirus based on JE vaccine virus represents a powerful tool to deliver foreign antigens.
    Print ISSN: 0022-538X
    Electronic ISSN: 1098-5514
    Topics: Medicine
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  • 10
    Publication Date: 2013-10-17
    Description: This paper reports an approach to enable rapid concentration and recovery of bacterial cells from aqueous chicken homogenates as a preanalytical step of detection. This approach includes biochemical pretreatment and prefiltration of food samples and development of an automated cell concentration instrument based on cross-flow microfiltration. A polysulfone hollow-fiber membrane module having a nominal pore size of 0.2 μm constitutes the core of the cell concentration instrument. The aqueous chicken homogenate samples were circulated within the cross-flow system achieving 500- to 1,000-fold concentration of inoculated Salmonella enterica serovar Enteritidis and naturally occurring microbiota with 70% recovery of viable cells as determined by plate counting and quantitative PCR (qPCR) within 35 to 45 min. These steps enabled 10 CFU/ml microorganisms in chicken homogenates or 10 2 CFU/g chicken to be quantified. Cleaning and sterilizing the instrument and membrane module by stepwise hydraulic and chemical cleaning (sodium hydroxide and ethanol) enabled reuse of the membrane 15 times before replacement. This approach begins to address the critical need for the food industry for detecting food pathogens within 6 h or less.
    Print ISSN: 0099-2240
    Electronic ISSN: 1098-5336
    Topics: Biology
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