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  • Gas chromatography  (3)
  • sarcoplasmic reticulum  (3)
  • Plasma renin concentration  (2)
  • Springer  (8)
  • American Heart Association (AHA)
  • National Academy of Sciences
  • 2010-2014
  • 1995-1999  (6)
  • 1970-1974  (2)
  • 1955-1959
Document type
Keywords
Publisher
  • Springer  (8)
  • American Heart Association (AHA)
  • National Academy of Sciences
Years
  • 2010-2014
  • 1995-1999  (6)
  • 1970-1974  (2)
  • 1955-1959
  • 1985-1989  (1)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Bestimmung der Plasma-Renin-Konzentration mittels Radioimmunoassays für Angiotensin I (1972)
    Springer
    Journal of molecular medicine 50 (1972), S. 702-705 
    Details
    ISSN: 1432-1440
    Keywords: Plasma renin concentration ; radioimmunoassay ; angiotensin I ; Plasma-Renin-Konzentration ; radioimmunologischer Nachweis ; Angiotensin I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Ein Radioimmunoassay für Angiotensin I und seine Anwendung für die Messung der Reninkonzentration im Plasma werden beschrieben. Die zur Herstellung von Angiotensinantikörpern und radioaktiv markiertem Angiotensin sowie zur Trennung von gebundenem und freiem Hormon benutzten Verfahren werden mitgeteilt. Die Empfindlichkeit der Methode erlaubt den Nachweis von zwanzig Pikogramm Angiotensin I. Zur Messung der Reninkonzentration wurde substratfreies Plasma mit Schafsubstrat im Überschuß versetzt und in Anwesenheit von Inhibitoren von „converting enzyme“ und Angiotensinasen bei 37°C inkubiert. Das gebildete Angiotensin wurde in 20 µl des proteinfreien Inkubationsgemisches bestimmt. Die initiale Geschwindigkeit der Angiotensinbildung wurde zur Berechnung der Reninkonzentration herangezogen. Als eine Einheit wurde die Reninmenge definiert, die ein Nanogramm Angiotensin I pro Stunde Inkubation bildet. Normalwerte unter kontrollierter natriumreicher und natriumarmer Diät wurden ermittelt. Die Empfindlichkeit der Methode erlaubt die Messung der Reninkonzentration im Plasma von Patienten mit primärem Aldosteronismus.
    Notes: Summary A radioimmunoassay for angiotensin I and its application to the measurement of plasma renin concentration are described. Outlined are the methods which were used to elicite antibodies against angiotensin I, to iodinate angiotensin I with iodine125, and to separate free from antibody-bound hormone. The method is sensitive enough to detect 20 picogrammes of angiotensin I. Substrate-free plasma was mixed with excess of sheep substrate. The mixture was incubated at 37°C in the presence of inhibitors of converting enzyme and angiotensinases. The generated angiotensin I was measured in 20 µl of the protein-free incubation mixture. The initial velocity of angiotensin generation was used to calculate the renin concentration. One unit was defined as the amount of renin which generates one nanogram of angiotensin per hour. Normal values of plasma renin concentration were obtained both during sodium loading and sodium depletion. The sensitivity of the method allows the measurement of plasma renin in patients with primary aldosteronism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01495529
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  • 2
    Electronic Resource
    Electronic Resource
    Die Plasma-Renin-Konzentration beim primären Hyperaldosteronismus unter Suppressions- und Stimulationsbedingungen (1972)
    Springer
    Journal of molecular medicine 50 (1972), S. 783-786 
    Details
    ISSN: 1432-1440
    Keywords: Primary aldosteronism ; Plasma renin concentration ; Primärer Hyperaldosteronismus ; Plasma-Renin-Konzentration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 13 Patienten mit einem primären Hyperaldosteronismus wurde die Plasma-Renin-Konzentration unter Stimulations- und unter Suppressionsbedingungen gemessen. Eine hochempfindliche Methode zur Reninmessung, die sich eines Radioimmunoassays für Angiotensin I bediente, wurde verwandt. Bei jedem der Patienten ließ sich die Reninkonzentration im Plasma gut messen. Die Werte lagen hochsignifikant unter den Normbereichen, die unter denselben Bedingungen ermittelt worden waren. Am eindrücklichsten war der Unterschied zwischen normalen und pathologischen Werten unter Stimulationsbedingungen. Nach Kochsalzentzug stieg die Reninkonzentration zwar bei nahezu allen Patienten signifikant an, der Anstieg war jedoch subnormal, gemessen an dem gesunden Kontrollkollektiv. Renin ist auch beim primären Aldosteronismus im Plasma vorhanden und reagiert auf Stimulation qualitativ regelrecht, quantitativ jedoch subnormal.
    Notes: Summary Plasma renin concentration (PRC) was measured in thirteen patients with primary aldosteronism during high and low sodium intake. A highly sensitive method using a radioimmunoassay for angiotensin I was applied for the determinations of PRC. PRC was detectable in each patient. The values obtained were significantly lower than the normal ranges which were assessed under the same conditions. Most impressive was the difference between normal and pathological values during sodium deprivation. PRC rose in almost each patient after several days of dietary sodium restriction. The increase was subnormal, however, when compared with normal controls. Renin is present in plasma of patients with primary aldosteronism. It responds qualitatively normally, but quantitatively subnormally when stimulated by sodium deprivation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01490305
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of different culture conditions on sarcoplasmic reticular calcium transport in isolated neonatal rat cardiomyocytes (1998)
    Springer
    Molecular and cellular biochemistry 188 (1998), S. 177-185 
    Details
    ISSN: 1573-4919
    Keywords: neonatal rat cardiomyocyte culture ; sarcoplasmic reticulum ; phospholamban ; calcium ATPase ; calcium transport ; thyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This study investigates sarcoplasmic reticulum (SR) calcium-(Ca2+) transport ATPase (SERCA2a) and phospholamban (PLB) in cultured spontaneously contracting neonatal rat cardiomyocytes (CM) to ascertain the function of both SR proteins under various culture conditions. The two major SR proteins were readily detectable in cultured CM by immunofluorescent microscopy using specific anti-SERCA2 and anti-PLB antibodies. Double labeling technique revealed that PLB-positive CM also labeled with anti-SERCA2. Coexpression of SERCA2 and PLB in CM was supported by measurement of cell homogenate oxalate-supported Ca2+ uptake which was completely inhibited by thapsigargin and stimulated by protein kinase A-catalyzed phosphorylation. Under serum-free conditions, incubation of CM with the SERCA2a expression modulator 3,3′,5-triiodo-L-thyronine (100 nM, 72 h) resulted in elevated Ca2+ uptake of +33%. Specific Ca2+ uptake activity was not altered if insulin was omitted from the serum-free culture medium but total SR Ca2+ transport activity was reduced under this culture condition. The results indicate that primary culture of spontaneously contracting neonatal rat CM can be employed as a useful model system for investigating both short- and long-term mechanisms determining the Ca2+ re-uptake function of the SR under defined culture conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006850724830
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  • 4
    Electronic Resource
    Electronic Resource
    Early postnatal changes in sarcoplasmic reticulum calcium transport function in spontaneously hypertensive rats (1996)
    Springer
    Molecular and cellular biochemistry 163-164 (1996), S. 57-66 
    Details
    ISSN: 1573-4919
    Keywords: heart ; postnatal development ; sarcoplasmic reticulum ; phospholamban ; calcium transport ; spontaneously hypertensive rats ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This comparative study investigates the relationship between sarcoplasmic reticulum (SR) calcium(Ca2+)-ATPase transport activity and phospholamban (PLB) phosphorylation in whole cardiac homogenates of spo`ntaneously hypertensive rats (SHR) and their parent, normotensive Wistar Kyoto (WKY) strain during early postnatal development at days 1, 3, 6, 12 and at day 40 to ascertain any difference in SR Ca2+ handling before the onset of hypertension. At day 1, the rate of homogenate oxalate-supported Ca2+ uptake was significantly higher in SHR than in WKY (0.25 ± 0.02 vs 0.12 ± 0.01 nmoles Ca2+/mg wet ventricular weight/min, respectively; p 〈 0.001). This interstrain difference disappeared with further developmental increase in SR Ca2+ transport. Western Blot analysis and a semiquantitative ELISA did not reveal any difference in the amount of immunoreactive PLB (per mg of total tissue protein) between strains at any of the ages studied. In addition, levels of phosphorylated PLB formed in vitro in the presence of radiolabelled ATP and catalytic (C) subunit of protein kinase A did not differ between SHR and WKY at days 1, 3, 6 and 12. At day 40, C subunit-catalyzed formation of 32P-PLB was reduced by 66% (p 〈 0.001) in SHR when compared to age-matched WKY In the early postnatal period between day 1 and 12 SR Ca2+-transport values were linearly related to the respective 32P-PLB levels of both SHR and WKY rats. The results indicate that cardiac SR of SHR can sequester Ca2+ at a much higher rate immediately after birth compared to WKY rats. The disappearance of this interstrain difference with further development suggests that some endogenous neuroendocrine or nutritional factor(s) from the hypertensive mother may exert an influence upon the developing heart in utero resulting in a transiently advanced maturation of the SR Ca2+ transport function in SHR pups at the time of birth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408641
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  • 5
    Electronic Resource
    Electronic Resource
    Mechanisms of thyroid hormone control over sensitivity and maximal contractile responsiveness to β-adrenergic agonists in atria (1998)
    Springer
    Molecular and cellular biochemistry 184 (1998), S. 419-426 
    Details
    ISSN: 1573-4919
    Keywords: atria ; thyroid hormones ; β-adrenergic effect ; sarcoplasmic reticulum ; phospholamban
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract This paper discusses the mechanisms of two basic effects of thyroid hormones on atrial responses to β-adrenergic agonists, i.e. increased inotropic sensitivity and decreased maximal contractile responsiveness. The increased sensitivity of atria to β-adrenergic agonists under thyroid hormones appears to be related to increases in β-adrenoceptor density and Gs/Gi protein ratio, leading to activation of Gs-mediated pathway, but suppression of Gi-mediated pathway of adenylate cyclase regulation. Therefore, the i/c concentrations of cAMP and corresponding inotropic responses achieve their maximums at lower doses of β-adrenergic agonist. Thyroid hormones also decrease the expression of phospholamban, but increase the expression of sarcoplasmic reticulum Ca+2-pump. As a result, the basal activity of sarcoplasmic reticulum Ca+2-pump increases, but its β-adrenergic activation through phosphorylation of phospholamban decreases. It is suggested that these changes are causal for decreased maximal inotropic and lusitropic responses of atria to β-adrenergic agonists.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006835213108
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  • 6
    Electronic Resource
    Electronic Resource
    Quantitative determination of toxic mono- and non-ortho polychlorinated biphenyls in cod liver oil after selective liquid chromatographic separation (1995)
    Springer
    Chromatographia 41 (1995), S. 585-593 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Column liquid chromatography ; Polychlorinated biphenyls ; Cod liver oil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary By application of a chromatographic column filled with Supelclean ENVI-Carb/Celite and elution with three solvents of different polarity three PCB fractions were obtained. Fraction A contained poly-ortho PCBs, Fraction B mono-ortho PCBs, and Fraction C non-ortho PCBs. The Supelclean ENVI-Carb/Celite column was used in combination with a sample preparation procedure for pre-cleaning of acid-stable chlorinated hydrocarbons such as DDT and its metabolites, HCH isomers, and regulation-relevant PCB congeners. The method was optimized using standard solutions of 55 PCB congeners, 8 chlorinated pesticides and contaminated cod liver oil samples. The influence of traces of remaining matrix on the elution profile of the organochlorine compounds on Supelclean ENVI-Carb/Celite was observed. Quantitation was carried out by GC-ECD with fused silica capillary columns of different polarity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02269724
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  • 7
    Electronic Resource
    Electronic Resource
    Fast and effective sample preparation for determination of organochlorine compounds in fatty tissue of marine mammals using microwave extraction (1996)
    Springer
    Chromatographia 42 (1996), S. 300-304 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Microwave extraction ; Sample clean-up ; Organochlorine compounds ; Blubber of marine mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A rapid and effective method is described for the extraction of organochlorine compounds (PCB 153, PCB 138, PCB 180, p,p′-DDE, α-HCH, γ-HCH, ε-HCH and HCB) from seal blubber and pork fat withn-hexane using a microwave technique. Heating of the non-polarn-hexane was achieved using a microwave transformer. The lipid content of the samples obtained by this extraction was identical to that by Soxhlet extraction. After separation of sample matrix and organochlorines on a silica gel column the organochlorine compounds were determined by GC-ECD. The efficiency of the method was tested with 500 mg spiked fat, extracted using various numbers of extraction cycles. Recoveries of organochlorine compounds in grey seal blubber and spiked pork fat generally exceeded 90 %.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02290313
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  • 8
    Electronic Resource
    Electronic Resource
    Enantioseparation of the compounds of technical toxaphene (CTTs) on 35% heptakis (6-O-tert-butyldimethylsilyl-2,3-di-O-Methyl)-β-cyclodextrin in OV1701 (1998)
    Springer
    Chromatographia 47 (1998), S. 565-569 
    Details
    ISSN: 1612-1112
    Keywords: Gas chromatography ; Chiral stationary phases ; Enantioseparation ; Toxaphene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The separation of the enantiomers of the compounds of technical toxaphene (CTTs) on heptakis(6-O-tert-butyldimethylsilyl-2,3-di-O-methyl)-β-cyclodextrin (β-TBDM) has been studied by gas chromatography with electron-capture detection (GC-ECD). Enantiomers of eight of the nine CTTs under investigation were separated on this chiral stationary phase. Separations of the enantioners of CTTs have hitherto been achieved ontert-butyldimethylsilylated β-cyclodextrin (β-BSCD). The chiral resolution values and separation factors of the CTTs on β-TBDM have been compared with those obtained on β-BSCD. Although several components coeluted, enantioselective determination of three CTTs was possible in an extract of seal blubber. For each CTT the first-eluting enantiomer was enantioenriched. Enantioselective accumulation of2-endo, 3-exo,5-endo,6-exo,-8,8,9,10-octachlorobornane (B8-1412) in biota has been established for the first time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02467496
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