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  • 2010-2014  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 7 (1960), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Three strains of Hypotrichomonas acosta were isolated in axenic culture. Attempts to develop a defined medium directly from a defined medium suitable for Tetrahymena pyriformis were unsuccessful. Development of partly defined media by substitution for undefined materials in Diamond's medium were more successful. Horse serum was replaced by 1 mg. % TEM-4T (a diacetyl tartaric acid ester of monoglycerides from tallow) and 0.5 mg.% cholesterol. Yeast extract was replaced by a mixture of ribonucleotides. Inclusion of several additional components permitted reducing the Trypticase concentration from 2% to 0.25%.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 17 (1970), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The life cycles of 2 strains of Allogromia laticollaris (CSH and TPA) raised on experimentally restricted diets have been studied. Cloned cultures of the strains differed in nutritional requirements for continuous reproduction. The TPA strain was more fecund. Both strains have a basically apogamic (asexual) life cycle; only occasionally were gamonts (sexually reproducing individuals) produced. They were autogamous (self-fertilizing) and fusion of the gametes took place within the parental test.We found in CSH clones a regular alternation of vesicularly nucleated and compactly multinucleated diploid generations. The details of the life cycle of either strain are not known well enough to be able to construct life cycle diagrams more meaningful than that of Arnold. The 2 strains studied differ in cytologic and nuclear detail from each other and from the strain studied by Arnold.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 16 (1969), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. The growth and reproduction of Allogromia laticollaris was studied. More schizozoites were generally produced in mixtures of food organisms than on single algal foods. In the presence of moderate numbers of bacteria, cultures with Phaeodactylum tricornutum, Chlorococcum sp., Nannochloris sp., and an unidentified chlorophyte (BL-1), added singly, were also highly productive. Schizogony was the dominant asexual form of reproduction. Binary fission and cytotomy also occurred in bacterized otherwise unfed controls. 35S and 32P are convenient labels for measuring growth of A. laticollaris when introduced into the system in the range of 1 × 104 - 1 × 105 dpm/ml (32P specific activity ∼ 2.03 MCi/g; 35S specific activity ∼ 95 μCi/g). Small allogromiids grew faster than did larger ones. By means of the Taylor series modification of the classical least-squares method, a continuous life-cycle representation was calculated for A. laticollaris for the conditions of the experiment. Four points of cell volume growth were maxima for reproduction: 1.0 × 107μ per organism for curve I; 2.2 × 107μ3 and 1.2 × 107μ3 for curve II; and 6.7 × 107μ3 for curve III.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 16 (1969), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Bacteria were required for the sustained reproduction of 4 species of foraminifera in gnotobiotic culture. None of the species of algae tested, singly or in combination, supported continuous reproduction of the foraminifera in bacteria-free gnotobiotic culture. It is inferred that bacteria have some nutritional factor required by the foraminifera that is either unavailable or unavailable in sufficient quantity in an exclusively algal diet.Gnotobiotic clones of Quinqueloculina lata, Spiroloculina hyalina, Rosalina leei, and Allogromia laticollaris were established on bacteria + algae (usually 1 or 2 species). In balanced gnotobiotic cultures neither light nor foraminiferan density (organisms/ml) were limiting. As cultures aged, pH shifted and limited growth. When waste products were removed by washing, reproductive rates were higher.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 9 (1962), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Several trichomonads from poikilotherms (Hypotrichomonas acosta, Monocercomonas colubrorum and M. sp., Trichomonas batrachorum, and Tritrichomonas augusta) grew in a nearly-defined medium to a level of 10–40% that permitted with media containing casein digests; T. augusta (4 strains) grew best. The defined medium contained TEM-4T (a semisynthetic fat based on beef-tallow fatty acids) and ascorbyl “palmitate” as sources of fatty acids. Besides ascorbyl palmitate, thiomalic and ascorbic acids, and tocopherol served as reducers and antiöxidants. High concentrations of nucleotides (adenylic, cytidylic, guanylic, and uridylic acids) seemed necessary. Indigo carmine was a useful redox indicator; the flagellates never grew in the oxidized zone. Extra trace elements and amino acids or rich natural media permitted better growth at about 34.5°C—the upper temperature limit for these trichomonads, raising the issue of a biochemical basis of a thermal barrier to the invasion of warmblooded hosts. Except for the limited carbohydrate in the nucleotides, carbohydrate could be omitted, the main substrates being a combination of glycerol, acetate, and gylcero-phosphate, posing the question of the identity of the anaerobic, presumably non-glycolytic, energy-yielding pathway.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 13 (1966), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Polysphondylium pallidum WS-320 grows indefinitely as vegetative amebae in a liquid medium where (a) substrates comprise sucrose, glycerol, acetate, lactate, citrate, and glutamate; (b) essential nutrients (riboflavin, lysine, glycine, and possibly several other amino acids that may be essential) are supplied. The growth thus supported (2 × 106 cells/ml) is more than doubled by provision of a mixture of crude fatty acids, an acid hydrolysate of casein supplemented with B vitamins, purines, pyrimidines, and fat-soluble antioxidants.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 27 (1980), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS The time is ripe for protozoan ecologists to begin the general evaluation of the role of food quality in the energy transformations and materials flow in food webs involving protozoa and other small organisms. Current evidence suggests that major pathways of energy flow at any particular time depend upon the matches between prey species and consumers. There are 2 components to food quality related (informational) energy flow: (a) the information present in the molecular constitution of the prey; and (b) the ability of the protozoa to recognize and use it. If the evidence obtained from trophodynamic studies of 2 marine ciliates, Uronema marinum Dujardin and Euplotes vannus Müller indicates a generalized ability of protozoa to regulate catabolic reactions to points which optimize energetic gains from their food, then it may be one of the keys to the evolutionary successes of the group.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 16 (1969), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A symbiotic alga from the foraminifer Archaias angulatus was isolated axenically. Algae from crushed hosts were coccoid and highly vacuolated; division stages within an envelope were common. Biflagellate motile piriform organisms predominated in newly transferred cultures and were gradually replaced by the coccoid, highly vacuolated stage. Incorporation of 14C label in intact Archaias was greatest for organisms fed and incubated in light. Starved symbiotized organisms incubated in the light incorporated ∼30% as much label as fed counterparts, There was no obvious difference in 45Ca incorporation between fed and starved organisms. Light significantly enhanced calcification. The Archaias symbiont infected Rosalina leei but not Quinqueloculina spp.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 13 (1966), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Tracer technic has proved to be an excellent tool in the study of predator-prey relationships among the foraminifera. More than fifty axenic species of protists including diatoms, dinoflagellates, chlorophytes, chrysophytes, cyanophytes, bacteria and yeasts were tested as potential food for Allogromia sp (NF), A. laticollaris, Am. monia beccarii, Quinqueloculina spp, Rosalina floridana, Anomalina sp, Elphidium sp, Spiroloculina hyalina, Globigerina bulloides, and Globorotalia truncatulinoides. Although many types of potential food are present in the environment, foraminifera select only certain organisms. The yeasts, cyanophytes, dinoflagellates, chrysophytes and most bacteria tested were not eaten. Selected species of diatoms, chlorophytes and bacteria were eaten in large quantity. Three additional factors affect feeding: the “age” of the food organism, the “age” of the foraminifer or its position in the life cycle, and the concentration of the food. Feeding by foraminifera on most food is erratic below a concentration of 103 organisms and is approximately directly proportional to concentration within a range of 103-106 organisms per 10 ml experimental tube. A natural bloom of Protelphidium tisburyensis was analyzed. A high concentration of 6 species of diatoms characterized the community. A “bloom”-feeder hypothesis for foraminiferal nutrition is presented.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 10 (1963), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. A polymorphic allogromiid (our strain NF) was isolated in monoxenic culture but attempts at its axenic culture failed. Growth of monoxenic cultures was stimulated by various metals and vitamins. The morphology and life cycle of this allogromiid with a prominent collar have been studied in detail. It varied in form from ovoid to elongate bioral “Shepheardella-like” forms and to irregular polyoral organisms. The organisms ranged in length from 56–385 μ (118 μ± 50.54) and in width from 35–385 μ (99 μ± 39.34). The number of nuclei per organism averaged 8.9 ± 6.3 (range 1–40). Reproduction of “Shepheardella-like” forms was by binary fission. Three types of budding have been observed and, rarely, schizogony. No evidence for sexual reproduction has thus far been seen.
    Type of Medium: Electronic Resource
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