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Heritable genome editing in C. elegans via a CRISPR-Cas9 system (2013)

Ari E Friedland; Yonatan B Tzur; Kevin M Esvelt; Monica P Colaiácovo; George M Church; John A Calarco

Nature Publishing Group (NPG)

In: Nature Methods

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Publication Date: 2013-07-31

Description: Nature Methods 10, 741 (2013). doi:10.1038/nmeth.2532 Authors: Ari E Friedland, Yonatan B Tzur, Kevin M Esvelt, Monica P Colaiácovo, George M Church & John A Calarco We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

Print ISSN: 1548-7091

Electronic ISSN: 1548-7105

Topics: Biology , Medicine

Published by Nature Publishing Group (NPG)

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Ecogenomic sensor reveals controls on N2-fixing microorganisms in the North Pacific Ocean (2014)

Julie C Robidart; Matthew J Church; John P Ryan; François Ascani; Samuel T Wilson; Deniz Bombar; Roman Marin; Kelvin J Richards; David M Karl; Christopher A Scholin; Jonathan P Zehr

Nature Publishing Group (NPG)

In: ISME Journal

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Publication Date: 2014-05-21

Description: Ecogenomic sensor reveals controls on N2-fixing microorganisms in the North Pacific Ocean The ISME Journal 8, 1175 (June 2014). doi:10.1038/ismej.2013.244 Authors: Julie C Robidart, Matthew J Church, John P Ryan, François Ascani, Samuel T Wilson, Deniz Bombar, Roman Marin, Kelvin J Richards, David M Karl, Christopher A Scholin & Jonathan P Zehr

Keywords: autonomous sensingbiosensorsdiazotrophsmicrobial oceanographynitrogen fixationtime-series

Print ISSN: 1751-7362

Electronic ISSN: 1751-7370

Topics: Biology

Published by Nature Publishing Group (NPG) on behalf of The International Society for Microbial Ecology (ISME).

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