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  • 2010-2014  (11)
  • 2005-2009  (2)
  • 2000-2004  (1)
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  • 1
    Book
    Book
    How does availability of allochthonous, dissolved organic matter (DOM) influence bacterial community composition in clear water and humic lakes? (2009)
    Details Availability
    Keywords: Hochschulschrift ; Landkreis Mecklenburgische Seenplatte ; Kohlendioxidbelastung ; Mikrobieller Abbau
    Type of Medium: Book
    Pages: XXVII, 178 S. , Ill., graph. Darst.
    DDC: 579.31714094315
    Language: English
    Note: Oldenburg, Univ., Diss., 2009
    Location Call Number Limitation Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Production of macroaggregates from dissolved exopolymeric substances (EPS) of bacterial and diatom origin (2005)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 53 (2005), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Exopolymeric substances (EPS) isolated from a pure culture of the marine bacterium Marinobacter sp. and the marine diatom Skeletonema costatum (axenic) were partially purified, chemically characterized and used as dissolved organic matter (DOM) for the production of macroaggregates. The role of organic particles such as transparent exopolymeric particles (TEP) and Coomassie stained particles (CSP) in the production of macroaggregates was experimentally assessed. Three experimental rolling tanks containing sterile medium with: (1) EPS, (2) EPS + live diatom cells and (3) EPS + killed bacteria, and three control tanks without any added EPS were used for macroaggregate production. Changes in abundance and average size of macroaggregates were monitored using image analysis, whereas TEP and CSP were enumerated microscopically. In the presence of microbial EPS, macroaggregates of a size of 23–35 mm2 were produced. Aggregate size and abundance considerably varied with both time and source of EPS. No correlation was observed for macroaggregate size and abundance with either TEP or CSP. One-way ANOVA demonstrated significant differences in the variance of particle abundance and size in tanks having only EPS or EPS in combination with live diatom cells. Our data suggest that production of macroaggregates was influenced by polymer chemistry and surface properties of colliding particles, whereas TEP and CSP concentrations were influenced by molecular weight of EPS and the presence of growing cells. Interestingly, macroaggregates were formed in the near absence of TEP and CSP, highlighting the role of other unknown processes in the transformation of DOM to particulate organic matter (POM) in aquatic environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/j.femsec.2004.12.013
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Antagonistic activity of bacteria isolated from organic aggregates of the German Wadden Sea (2004)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology ecology 47 (2004), S. 0 
    Details
    ISSN: 1574-6941
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Marine aggregates are densely colonized by bacteria, and inter-specific interactions such as inhibition are important for colonization by aggregate-associated bacteria and thus affect the turnover of organic matter in the sea. In order to study antagonistic activities we carried out inhibition tests with 51 isolates obtained exclusively from aggregates of the German Wadden Sea. 16S rRNA gene sequences of all isolates revealed that 35% of the isolates affiliated with the Flavobacteria/Sphingobacteria group, 24% and 16% with α- and γ-Proteobacteria, respectively, 16% with the Bacillus/Clostridium group, and 10% with Actinobacteria. The relatively high percentage of Gram-positive bacteria may be related to specific features of the Wadden Sea environment. After 11 days of incubation using Burkholder agar diffusion assays the percentage of inhibitory isolates was 54.1% and this decreased to 20.7% after 20 days of incubation but it did not decline for members of the Bacillus/Clostridium group. Inhibitory activity was expressed in strain-specific patterns even though some isolates were closely related according to their 16S rRNA gene sequences. Antagonistic activity was lowest for Flavobacteria/Sphingobacteria (35%) and highest for Actinobacteria (80%). We further examined whether growth of isolates was affected when they were placed on lawns of certain other isolates. In parallel with lowest percentage of inhibitory isolates, highest growth occurred on lawns of the Flavobacteria/Sphingobacteria group whereas it was lowest on lawns of Actinobacteria and the Bacillus/Clostridium group. The high inhibitory activity of both groups of Gram-positive bacteria fits well with data from chemical screening using matrix-assisted laser desorption ionization time of flight mass spectrometry. Hence, inhibitory activity greatly influences inter-specific interactions and may impact microbial degradation and remineralization of particulate organic matter in aquatic environments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0168-6496(03)00305-2
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    Paper (German National Licenses)
    Fulltext
  • 4
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    Dynamics of extracellular enzyme activities in seawater under changed atmospheric pCO2: a mesocosm investigation (2011)
    Inter Research
    In:  Aquatic Microbial Ecology, 64 (3). pp. 285-298.
    Details
    Publication Date: 2015-09-21
    Description: As part of the PeECE II mesocosm project, we investigated the effects of pCO2 levels on the initial step of heterotrophic carbon cycling in the surface ocean. The activities of microbial extracellular enzymes hydrolyzing 4 polysaccharides were measured during the development of a natural phytoplankton bloom under pCO2 conditions representing glacial (190 µatm) and future (750 µatm) atmospheric pCO2. We observed that (1) chondroitin hydrolysis was variable throughout the pre-, early- and late-bloom phases, (2) fucoidanase activity was measurable only in the glacial mesocosm as the bloom developed, (3) laminarinase activity was low and constant, and (4) xylanase activity declined as the bloom progressed. Concurrent measurements of microbial community composition, using denaturing-gradient gel electrophoresis (DGGE), showed that the 2 mesocosms diverged temporally, and from one another, especially in the late-bloom phase. Enzyme activities correlated with bloom phase and pCO2, suggesting functional as well as compositional changes in microbial communities in the different pCO2 environments. These changes, however, may be a response to temporal changes in the development of phytoplankton communities that differed with the pCO2 environment. We hypothesize that the phytoplankton communities produced dissolved organic carbon (DOC) differing in composition, a hypothesis supported by changing amino acid composition of the DOC, and that enzyme activities responded to changes in substrates. Enzyme activities observed under different pCO2 conditions likely reflect both genetic and population-level responses to changes occurring among multiple components of the microbial loop.
    Type: Article , PeerReviewed
    Format: text
    DOI: 10.3354/ame01522
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    OceanRep: Article in a Scientific Journal - peer-reviewed
  • 5
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    (Table 1) Total carbon content, alkalinity, pH and pCO2 of the experimental carbonate system during time (2012)
    PANGAEA
    Details
    Publication Date: 2023-03-14
    Keywords: Alkalinity, total; Alkalinity, total, standard deviation; BIOACID; Biological Impacts of Ocean Acidification; Calculated using CO2SYS; Carbon, total; Carbon, total, standard deviation; Carbon dioxide, partial pressure; Carbon dioxide, partial pressure, standard deviation; Colorimetry; DATE/TIME; Incubation duration; pH; pH, standard deviation; pH meter KNICK Model 761
    Type: Dataset
    Format: text/tab-separated-values, 120 data points
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    DATA PANGAEA
  • 6
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    Biotic and abiotic parameters and cynobacteria abundance during an experimental set-up with varying pCO2 conditions (2012)
    PANGAEA
    In:  Supplement to: Wannicke, Nicola; Endres, Sonja; Engel, Anja; Grossart, Hans-Peter; Unger, Juliane; Voss, Maren (2012): Response of Nodularia spumigena to pCO2 - Part 1: Growth, production and nitrogen cycling. Biogeosciences, 9(8), 2973-2988, https://doi.org/10.5194/bg-9-2973-2012
    Details
    Publication Date: 2023-05-12
    Description: Heterocystous cyanobacteria of the genus Nodularia form extensive blooms in the Baltic Sea and contribute substantially to the total annual primary production. Moreover, they dispense a large fraction of new nitrogen to the ecosystem when inorganic nitrogen concentration in summer is low. Thus, it is of ecological importance to know how Nodularia will react to future environmental changes, in particular to increasing carbon dioxide (CO2) concentrations and what consequences there might arise for cycling of organic matter in the Baltic Sea. Here, we determined carbon (C) and dinitrogen (N2) fixation rates, growth, elemental stoichiometry of particulate organic matter and nitrogen turnover in batch cultures of the heterocystous cyanobacterium Nodularia spumigena under low (median 315 µatm), mid (median 353 µatm), and high (median 548 µatm) CO2 concentrations. Our results demonstrate an overall stimulating effect of rising pCO2 on C and N2 fixation, as well as on cell growth. An increase in pCO2 during incubation days 0 to 9 resulted in an elevation in growth rate by 84 ± 38% (low vs. high pCO2) and 40 ± 25% (mid vs. high pCO2), as well as in N2 fixation by 93 ± 35% and 38 ± 1%, respectively. C uptake rates showed high standard deviations within treatments and in between sampling days. Nevertheless, C fixation in the high pCO2 treatment was elevated compared to the other two treatments by 97% (high vs. low) and 44% (high vs. mid) at day 0 and day 3, but this effect diminished afterwards. Additionally, elevation in carbon to nitrogen and nitrogen to phosphorus ratios of the particulate biomass formed (POC : POP and PON : POP) was observed at high pCO2. Our findings suggest that rising pCO2 stimulates the growth of heterocystous diazotrophic cyanobacteria, in a similar way as reported for the non-heterocystous diazotroph Trichodesmium. Implications for biogeochemical cycling and food web dynamics, as well as ecological and socio-economical aspects in the Baltic Sea are discussed.
    Keywords: BIOACID; Biological Impacts of Ocean Acidification
    Type: Dataset
    Format: application/zip, 3 datasets
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    DATA PANGAEA
  • 7
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    Impact of CO2 enrichment on organic matter dynamics during nutrient induced coastal phytoplankton blooms (2014)
    PANGAEA
    In:  GEOMAR - Helmholtz Centre for Ocean Research Kiel | Supplement to: Engel, Anja; Piontek, Judith; Grossart, Hans-Peter; Riebesell, Ulf; Schulz, Kai Georg; Sperling, Martin (2014): Impact of CO2 enrichment on organic matter dynamics during nutrient induced coastal phytoplankton blooms. Journal of Plankton Research, 36(3), 641-657, https://doi.org/10.1093/plankt/fbt125
    Details
    Publication Date: 2024-02-01
    Description: A mesocosm experiment was conducted to investigate the impact of rising fCO2 on the build-up and decline of organic matter during coastal phytoplankton blooms. Five mesocosms (~38 m³ each) were deployed in the Baltic Sea during spring (2009) and enriched with CO2 to yield a gradient of 355-862 µatm. Mesocosms were nutrient fertilized initially to induce phytoplankton bloom development. Changes in particulate and dissolved organic matter concentrations, including dissolved high-molecular weight (〉1 kDa) combined carbohydrates, dissolved free and combined amino acids as well as transparent exopolymer particles (TEP), were monitored over 21 days together with bacterial abundance, and hydrolytic extracellular enzyme activities. Overall, organic matter followed well-known bloom dynamics in all CO2 treatments alike. At high fCO2, higher dPOC:dPON during bloom rise, and higher TEP concentrations during bloom peak, suggested preferential accumulation of carbon-rich components. TEP concentration at bloom peak was significantly related to subsequent sedimentation of particulate organic matter. Bacterial abundance increased during the bloom and was highest at high fCO2. We conclude that increasing fCO2 supports production and exudation of carbon-rich components, enhancing particle aggregation and settling, but also providing substrate and attachment sites for bacteria. More labile organic carbon and higher bacterial abundance can increase rates of oxygen consumption and may intensify the already high risk of oxygen depletion in coastal seas in the future.
    Keywords: BIOACID; Biological Impacts of Ocean Acidification; SOPRAN; Surface Ocean Processes in the Anthropocene
    Type: Dataset
    Format: application/zip, 2 datasets
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    DATA PANGAEA
  • 8
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    Station list and links to master tracks in different resolutions of HEINCKE cruise HE299 (2014)
    PANGAEA
    In:  Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
    Details
    Publication Date: 2024-01-12
    Keywords: AWI_Paleo; Calculated; Course; CT; DATE/TIME; HE299; HE299-track; Heincke; LATITUDE; LONGITUDE; North Sea; Paleoenvironmental Reconstructions from Marine Sediments @ AWI; Speed; Underway cruise track measurements
    Type: Dataset
    Format: text/tab-separated-values, 1438 data points
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    DATA PANGAEA
  • 9
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    Station list and links to master tracks in different resolutions of HEINCKE cruise HE320 (2014)
    PANGAEA
    In:  Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven
    Details
    Publication Date: 2024-01-12
    Keywords: AWI_Paleo; Calculated; Course; CT; DATE/TIME; HE320; HE320-track; Heincke; LATITUDE; LONGITUDE; North Sea; Paleoenvironmental Reconstructions from Marine Sediments @ AWI; Speed; Underway cruise track measurements
    Type: Dataset
    Format: text/tab-separated-values, 1438 data points
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    DATA PANGAEA
  • 10
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    (Table 2) Dissolved organic and inorganic nutrients, chlorophyll a, and bacteria abundance under experimental pCO2 conditions (2012)
    PANGAEA
    Details
    Publication Date: 2024-03-08
    Keywords: Bacteria, abundance; Bacteria, abundance, standard deviation; BIOACID; Biological Impacts of Ocean Acidification; Carbon, organic, dissolved; Carbon, organic, dissolved, standard deviation; Chlorophyll a; Chlorophyll a, standard deviation; Counting by flow cytometer; DATE/TIME; Fluorometry (TURNER, 10-AU-005); Nitrogen, inorganic, dissolved; Nitrogen, inorganic, dissolved, standard deviation; Nitrogen, organic, dissolved; Nitrogen, organic, standard deviation; Phosphate; Phosphate, standard deviation; Phosphorus, organic, dissolved; Phosphorus, organic, standard deviation; Spectrophotometer Hitachi U-2000; TOC analyzer (Shimadzu); Treatment
    Type: Dataset
    Format: text/tab-separated-values, 180 data points
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    DATA PANGAEA
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