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  • 2015-2019  (2)
  • 1
    Publication Date: 2016-07-02
    Description: Cryptochromes constitute a group of flavin-binding blue light receptors in bacteria, fungi, plants, and insects. Recently, the response of cryptochromes to light was extended to nearly the entire visible spectral region on the basis of the activity of the animal-like cryptochrome aCRY in the green alga Chlamydomonas reinhardtii. This finding was explained by the absorption of red light by the flavin neutral radical as the dark state of the receptor, which then forms the anionic fully reduced state. In this study, time-resolved UV-visible spectroscopy on the full-length aCRY revealed an unusually long-lived tyrosyl radical with a lifetime of 2.6 s, which is present already 1 μs after red light illumination of the flavin radical. Mutational studies disclosed the tyrosine 373 close to the surface to form the long-lived radical and to be essential for photoreduction. This residue is conserved exclusively in the sequences of other putative aCRY proteins distinguishing them from conventional (6–4) photolyases. Size exclusion chromatography showed the full-length aCRY to be a dimer in the dark at 0.5 mm injected concentration with the C-terminal extension as the dimerization site. Upon illumination, partial oligomerization was observed via disulfide bridge formation at cysteine 482 in close proximity to tyrosine 373. The lack of any light response in the C-terminal extension as evidenced by FTIR spectroscopy differentiates aCRY from plant and Drosophila cryptochromes. These findings imply that aCRY might have evolved a different signaling mechanism via a light-triggered redox cascade culminating in photooxidation of a yet unknown substrate or binding partner.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
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  • 2
    Publication Date: 2015-07-02
    Description: We present a time series of high-resolution spectra of the Type Ia supernova 2014J, which exploded in the nearby galaxy M82. The spectra were obtained with the HEROS échelle spectrograph installed at the 1.2-m TIGRE telescope. We present a series of 33 spectra with a resolution of R   20 000, which covers the important bright phases in the evolution of SN 2014J during the period from 2014 January 24 to April 1. The spectral evolution of SN 2014J is derived empirically. The expansion velocities of the Si  ii P-Cygni features were measured and show the expected decreasing behaviour, beginning with a high velocity of 14 000 km s –1 on January 24. The Ca  ii infrared triplet feature shows a high-velocity component with expansion velocities of 〉20 000 km s –1 during the early evolution apart from the normal component showing similar velocities as Si  ii . Further broad P-Cygni profiles are exhibited by the principal lines of Ca  ii , Mg  ii and Fe  ii . The TIGRE SN 2014J spectra also resolve several very sharp Na  i D doublet absorption components. Our analysis suggests interesting substructures in the interstellar medium of the host galaxy M82, as well as in our Milky Way, confirming other work on this SN. We were able to identify the interstellar absorption of M82 in the lines of Ca  ii H & K at 3933 and 3968 Å as well as K  i at 7664 and 7698 Å. Furthermore, we confirm several diffuse interstellar bands, at wavelengths of 6196, 6283, 6376, 6379and 6613 Å and give their measured equivalent widths.
    Print ISSN: 0035-8711
    Electronic ISSN: 1365-2966
    Topics: Physics
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