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  • 1
    Keywords: Forschungsbericht ; Medizinische Ethik ; Therapie ; Lebensqualität
    Type of Medium: Online Resource
    Pages: 1 Online-Ressource (5 Seiten, 93,33 KB)
    Language: German
    Note: Förderkennzeichen BMBF 01GP1383 , Durchführende Instititutionen und Ansprechpartner dem Berichtsblatt entnommen. - Konferenzdatum ermittelt , Unterschiede zwischen dem gedruckten Dokument und der elektronischen Ressource können nicht ausgeschlossen werden
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  • 2
    Publication Date: 2020-01-02
    Description: Aims: Airway and pulmonary vascular remodeling is an important pathological feature in the pathogenesis of chronic obstructive pulmonary disease (COPD). Tobacco smoke (TS) induces the production of large amounts of reactive oxygen species (ROS) in COPD lungs. We investigated how ROS lead to airway and pulmonary vascular remodeling in COPD. Results: We used in vitro bronchial and pulmonary artery smooth muscle cells (BSMCs and PASMCs), in vivo TS-induced COPD rodent models and lung tissues of COPD patients. We found that H2O2 and TS extract (TSE) induced calpain activation in BSMCs and PASMCs. Calpain activation was elevated in smooth muscle of bronchi and pulmonary arterioles in COPD patients and TS-induced COPD rodent models. Calpain inhibition attenuated H2O2- and TSE-induced collagen synthesis and proliferation in BSMCs and PASMCs. Exposure to TS causes increases in airway resistance, right ventricular systolic pressure (RVSP) and thickening of bronchi and pulmonary arteries. Calpain inhibition by smooth muscle-specific knockout of calpain and the calpain inhibitor MDL28170 attenuated increases in airway resistance, RVSP and thickening of bronchi and pulmonary arteries. Moreover, smooth muscle-specific knockout of calpain did not reduce TS-induced emphysema in the mouse model, but MDL28170 did reduce TS-induced emphysema in the rat model. Innovation: This study provides the first evidence that ROS-induced calpain activation contributes to airway and pulmonary vascular remodeling in TS-induced COPD. Calpain might be a novel therapeutic target for the treatment of COPD. Conclusion: These results indicate that ROS-induced calpain activation contributes to airway and pulmonary vascular remodeling and pulmonary hypertension in COPD.
    Type: Article , PeerReviewed
    Format: text
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  • 3
    Publication Date: 2016-12-22
    Description: Our geological heritage is increasingly threatened by anthropogenic activity. This is especially true of the Pál-völgyi Cave System beneath Budapest. It is among the 150 longest and at the same time most endangered cave systems in the world. The aims of the study were (i) to set up a monitoring system in the cave, (ii) to track the daily changes in the quality and quantity of drip water, and (iii) to determine the exposure of the cave. Monitoring was conducted at two locations in a shallow area next to a fracture zone (site name: TG) and one lying in a tectonically less disturbed, geologically more homogeneous location 20 m deeper (site YC). The data obtained in respect of 13 variables were assessed using descriptive statistics, principal component- and periodicity analyses. At first glance, it was apparent that the eight water quality parameters differed in quantity between the two sites. Furthermore, using principal component analysis it was shown that in the fractured-shallow setting, anthropogenic activity (external urban pollution, e.g. de-icing, decrease of land cover etc.) is the driving process determining water quality. At the tectonically less fractured site (YC) external influences originating above ground may be added to the natural karst-forming processes. The assessment of drip intensity and electric conductivity again highlighted the differences between the sites in terms of their reaction to precipitation. With regard to diurnal periodicity, although pH and Eh indicated a mature periodic behavior at both sites (covering 56−65 % of the total observed time), at site TG electric conductivity displayed diurnal periodicity over only 21 % of the total time, compared to 56 % at YC. All results pointed towards a conclusion that at site YC daily periodicity and water quality are much more connected to natural processes, while at site TG anthropogenic external influences suppress these. Key words: cave drip water, hydrochemistry, karst, Pál-völgyi Cave System, time series analysis, urban pollution.   Analiza prenikle vode v urbani kraški jami pod madžarsko prestolnico (Budimpešta) Naša geološka dediščina je vse bolj ogrožena zaradi človekovih dejavnosti. To še posebej velja za jamski sistem Pál-völgyi pod Budimpešto. Sodi med 150 najdaljših in hkrati najbolj ogroženih jamskih sistemov na svetu. Cilji raziskave so bili (i) vzpostaviti sistem spremljanja izbranih parametrov v jami, (ii) spremljati dnevne spremembe kakovosti in količine prenikle vode, in (iii) določiti izpostavljenost jame onesnaženju. Raziskava je bila izvedena na dveh lokacijah v plitvem območju blizu razpoklinske cone (ime vzorčnega mesta: TG) in na tektonsko manj pretrti in geološko bolj homogeni lokaciji 20 m globlje (ime vzorčnega mesta: YC). Pridobljeni podatki 13 spremenljivk so bili obdelani s pomočjo opisne statistike, analize glavnih komponent in periodičostno analizo. Na prvi pogled je bilo očitno, da se je osem parametrov kakovosti vode kvantitativno razlikovalo med obema mestoma. Poleg tega se je s pomočjo analize glavnih komponent pokazalo, da je mesto v plitvem pretrtem območju pod velikim vplivom antropogenih aktivnosti (urbano onesnaženje, npr. sredstva proti zmrzovanju, zmanjšanje pokrovnih plasti, itd.), ki vplivajo na kakovost vode. Na tektonsko manj preoblikovanem mestu (YC) se zunanji vplivis površja mešajo z naravnimi kraškimi procesi.  Ocena intenzivnosti kapljanja in električne prevodnosti je ponovno izpostavila razlike med vzorčnimi mesti v smislu njunega odziva na padavine. V zvezi z dnevno periodičnostjo je vzorčno mesto TG kazalo dnevno periodičnost več kot 21 % celotnega časa, mesto YC 56 %, čeprav sta pH in Eh izkazali zrelo periodično obnašanje (56−65 % celotnega opazovanega časa) na obeh mestih. Vsi rezultati kažejo na sklep, da je na mestu YC dnevna periodičnost in kakovost vode veliko bolj povezana z naravnimi procesi, medtem ko jih na mestu TG antropogeni zunanji vplivi zadušijo. Ključne besede: jamska prenikla voda, hidrokemija, kras, jamski sistem Pál-völgyi, analiza časovne vrste, urbano onesnaževanje.  
    Print ISSN: 0583-6050
    Electronic ISSN: 1580-2612
    Topics: Geosciences
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