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  • 2015-2019  (6)
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  • 1
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    Seawater carbonate chemistry and phytoplankton biomass and species composition of a unique temperate rocky coastal hydrothermal vent system (2019)
    PANGAEA
    Details
    Publication Date: 2024-03-15
    Description: In situ effects of ocean acidification are increasingly studied at submarine CO2 vents. Here we present a preliminary investigation into the water chemistry and biology of cool temperate CO2 vents near Whakaari–White Island, New Zealand. Water samples were collected inside three vent shafts, within vents at a distance of 2 m from the shaft and at control sites. Vent samples contained both seawater pH on the total scale (pHT) and carbonate saturation states that were severely reduced, creating conditions as predicted for beyond the year 2100. Vent samples showed lower salinities, higher temperatures and greater nutrient concentrations. Sulfide levels were elevated and mercury levels were at concentrations considered toxic at all vent and control sites, but stable organic and inorganic ligands were present, as deduced from Cu speciation data, potentially mediating harmful effects on local organisms. The biological investigations focused on phytoplankton, zooplankton and macroalgae. Interestingly, we found lower abundances but higher diversity of phytoplankton and zooplankton at sites in the direct vicinity of Whakaari. Follow-up studies will need a combination of methods and approaches to attribute observations to specific drivers. The Whakaari vents represent a unique ecosystem with considerable biogeochemical complexity, which, like many other vent systems globally, require care in their use as a model of 'future oceans'.
    Keywords: Alkalinity, total; Ammonium; Ammonium, standard deviation; Aragonite saturation state; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Cell density; CO2 vent; Coast and continental shelf; Community composition and diversity; Entire community; Equitability; Field measurement; Field observation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Nitrogen oxide; Nitrogen oxide, standard deviation; Number of species; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pelagos; pH; Phosphate; Phosphate, standard deviation; Salinity; Shannon Diversity Index; South Pacific; Station label; Temperate; Temperature, water; Type; Whakaari_White_Island
    Type: Dataset
    Format: text/tab-separated-values, 241 data points
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    DATA PANGAEA
  • 2
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    The effects of diet and feeding techniques on growth factors and meat quality of common carp (Cyprinus carpio) (2018)
    Details
    Publication Date: 2021-05-19
    Description: The study was conducted for 134 days. Common carp fry (60g ± 0.3g) were fed using two different diets containing only plant protein sources, without any animal protein inclusions. Diets were administered by two different methods: an automatic band feeder (diet 28/7 F and 28/15 F) for 12 hours a day (9.00 a.m. – 9.00 p.m.) and by hand (diet 28/7 H and 28/15 H) once a day (at 9 am). Weight, Specific Growth Rate (SGR), Protein Efficiency Ratio (PER), Fat Retention Index (FR) and Protein Retention Index (PR) were statistically higher in fish fed with the automatic feeder. On the contrary, the value of FCR was statistically higher in fish fed by hand. Diet composition did not have significant influence on growth performance. Survival rate was not statistically different among groups (85-96%). There was no significant effect of feeding method or diet on meat quality, such as color and fat content.
    Description: Published
    Keywords: Cyprinus carpio ; Automatic feeders ; Common carp ; Feeding by hand ; Feeding techniques ; Plant protein sources ; Quality ; Meet ; Feeding ; Diet
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.639-653
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    PAPER (OA)
  • 3
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    Methamphetamine use in Central Germany: protocol for a qualitative study exploring requirements and challenges in healthcare from the professionals' perspective (2016)
    BMJ Publishing
    In: BMJ Open
    Details
    Publication Date: 2016-06-04
    Description: Introduction The synthetic drug methamphetamine with its high addiction potential is associated with substantial adverse health effects. In Germany, especially Central Germany, the increase in the consumption of methamphetamine has exceeded that of other illegal drugs. The treatment system and service providers are facing new challenges due to this rise in consumption. This qualitative study will explore the demand created by the increasing healthcare needs of methamphetamine-addicted persons in Central Germany, and the difficulty of rehabilitating addicted people. Methods and analysis The collection of empirical data will take place in a consecutive, two-stage process. In the first part of data collection, the experiences and perspectives of 40 professionals from numerous healthcare sectors for methamphetamine-addicted persons will be explored with the help of semistructured face-to-face interviews and probed by the research team. These findings will be discussed in 2 focus groups consisting of the participants of the face-to-face interviews; these group discussions comprise the second part of the data collection process. The interviews will be audio recorded, transcribed, and then subjected to qualitative content analysis. Ethics and dissemination All interviewees will receive comprehensive written information about the study, and sign a declaration of consent prior to the interview. The study will comply rigorously with data protection legislation. The research team has obtained the approval of the Ethical Review Committee at the Martin Luther University Halle-Wittenberg, Germany. The results of the study will be published in high-quality, peer-reviewed international journals, presented at several congresses and used to design follow-up research projects. Trial registration number VfD_METH_MD_15_003600.
    Keywords: Open access, Addiction, Health services research, Qualitative research, Rehabilitation medicine
    Electronic ISSN: 2044-6055
    Topics: Medicine
    Published by BMJ Publishing
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    PAPER CURRENT
  • 4
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    Low-light satellite sees glowing atmospheric waves [Environmental Sciences] (2015)
    National Academy of Sciences
    Details
    Publication Date: 2015-12-09
    Description: Gravity waves (disturbances to the density structure of the atmosphere whose restoring forces are gravity and buoyancy) comprise the principal form of energy exchange between the lower and upper atmosphere. Wave breaking drives the mean upper atmospheric circulation, determining boundary conditions to stratospheric processes, which in turn influence tropospheric weather...
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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    PAPER CURRENT
  • 5
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    Protein phosphorylation and its role in archaeal signal transduction (2016)
    Oxford University Press
    Details
    Publication Date: 2016-09-02
    Description: Reversible protein phosphorylation is the main mechanism of signal transduction that enables cells to rapidly respond to environmental changes by controlling the functional properties of proteins in response to external stimuli. However, whereas signal transduction is well studied in Eukaryotes and Bacteria, the knowledge in Archaea is still rather scarce. Archaea are special with regard to protein phosphorylation, due to the fact that the two best studied phyla, the Euryarchaeota and Crenarchaeaota, seem to exhibit fundamental differences in regulatory systems. Euryarchaeota (e.g. halophiles, methanogens, thermophiles), like Bacteria and Eukaryotes, rely on bacterial-type two-component signal transduction systems (phosphorylation on His and Asp), as well as on the protein phosphorylation on Ser, Thr and Tyr by Hanks-type protein kinases. Instead, Crenarchaeota (e.g. acidophiles and (hyper)thermophiles) only depend on Hanks-type protein phosphorylation. In this review, the current knowledge of reversible protein phosphorylation in Archaea is presented. It combines results from identified phosphoproteins, biochemical characterization of protein kinases and protein phosphatases as well as target enzymes and first insights into archaeal signal transduction by biochemical, genetic and polyomic studies.
    Print ISSN: 0168-6445
    Electronic ISSN: 1574-6976
    Topics: Biology
    Published by Oxford University Press
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    PAPER CURRENT
  • 6
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    Translesion Synthesis Past 7-Guanine DNA-Protein Conjugates [DNA and Chromosomes] (2016)
    The American Society for Biochemistry and Molecular Biology (ASBMB)
    Details
    Publication Date: 2016-11-05
    Description: DNA-protein cross-links (DPCs) are bulky DNA lesions that form both endogenously and following exposure to bis-electrophiles such as common antitumor agents. The structural and biological consequences of DPCs have not been fully elucidated due to the complexity of these adducts. The most common site of DPC formation in DNA following treatment with bis-electrophiles such as nitrogen mustards and cisplatin is the N7 position of guanine, but the resulting conjugates are hydrolytically labile and thus are not suitable for structural and biological studies. In this report, hydrolytically stable structural mimics of N7-guanine-conjugated DPCs were generated by reductive amination reactions between the Lys and Arg side chains of proteins/peptides and aldehyde groups linked to 7-deazaguanine residues in DNA. These model DPCs were subjected to in vitro replication in the presence of human translesion synthesis DNA polymerases. DPCs containing full-length proteins (11–28 kDa) or a 23-mer peptide blocked human polymerases η and κ. DPC conjugates to a 10-mer peptide were bypassed with nucleotide insertion efficiency 50–100-fold lower than for native G. Both human polymerase (hPol) κ and hPol η inserted the correct base (C) opposite the 10-mer peptide cross-link, although small amounts of T were added by hPol η. Molecular dynamics simulation of an hPol κ ternary complex containing a template-primer DNA with dCTP opposite the 10-mer peptide DPC revealed that this bulky lesion can be accommodated in the polymerase active site by aligning with the major groove of the adducted DNA within the ternary complex of polymerase and dCTP.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
    Published by The American Society for Biochemistry and Molecular Biology (ASBMB)
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    PAPER CURRENT
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