In:
Advanced Healthcare Materials, Wiley, Vol. 6, No. 18 ( 2017-09)
Abstract:
Extracellularmatrix (ECM)‐based scaffolds are important for their potential therapeutic application. Treated dentin matrix (TDM), a kind of ECM, seeded with allogeneic dental follicle stem cells (TDM/aDFC) provides a suitable inductive microenvironment for tooth root regeneration. Considering the limited sources, xenogeneic TDM (xTDM) is a possible alternative to allogeneic TDM; however, xTDM‐based scaffold presents severe osteolysis and resorption lacunae causing regenerated tooth root failure. Immune response–induced excessive osteoclastogenesis plays a critical role in xenogeneic scaffold osteolysis and resorption. The impact of antioxidant, tert ‐butylhydroquinone ( t BHQ), on xTDM/aDFCs‐induced osteoclastogenesis and osteoclastic resorption in vivo and in vitro are investigated. t BHQ upregulates heme oxygenase‐1 release and downregulates high mobility group box 1 mRNA expression. mRNA expression of other osteoclast‐related genes including nuclear factor‐kappa Bp65, receptor activator of nuclear factor kappa‐B, nuclear factor of activated T‐cells cytoplasmic 1, cathepsin K, and integrin β3, also decreases significantly. Furthermore, t BHQ‐treated xTDM/aDFCs scaffolds implanted into rhesus macaques show reduced osteolysis and osteoclastic resorption by microcomputed tomography and tartrate‐resistant acid phosphatase staining. t BHQ‐induced suppression of xTDM/aDFC‐induced osteoclastogenesis and osteoclastic resorption presents a new strategy for the regeneration of biological tooth root and could be applied to the regeneration of other complex tissues and organs.
Type of Medium:
Online Resource
ISSN:
2192-2640
,
2192-2659
DOI:
10.1002/adhm.201700127
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2645585-7
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