In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-01-06)
Abstract:
Centromeres are unique chromosomal loci that promote the assembly of kinetochores, macromolecular complexes that bind spindle microtubules during mitosis. In most organisms, centromeres lack defined genetic features. Rather, they are specified epigenetically by a centromere-specific histone H3 variant, CENP-A. The Mis18 complex, comprising the Mis18α:Mis18β subcomplex and M18BP1, is crucial for CENP-A homeostasis. It recruits the CENP-A-specific chaperone HJURP to centromeres and primes it for CENP-A loading. We report here that a specific arrangement of Yippee domains in a human Mis18α:Mis18β 4:2 hexamer binds two copies of M18BP1 through M18BP1’s 140 N-terminal residues. Phosphorylation by Cyclin-dependent kinase 1 (CDK1) at two conserved sites in this region destabilizes binding to Mis18α:Mis18β, limiting complex formation to the G1 phase of the cell cycle. Using an improved viral 2A peptide co-expression strategy, we demonstrate that CDK1 controls Mis18 complex recruitment to centromeres by regulating oligomerization of M18BP1 through the Mis18α:Mis18β scaffold.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.23352.001
DOI:
10.7554/eLife.23352.002
DOI:
10.7554/eLife.23352.003
DOI:
10.7554/eLife.23352.004
DOI:
10.7554/eLife.23352.005
DOI:
10.7554/eLife.23352.006
DOI:
10.7554/eLife.23352.007
DOI:
10.7554/eLife.23352.008
DOI:
10.7554/eLife.23352.009
DOI:
10.7554/eLife.23352.010
DOI:
10.7554/eLife.23352.011
DOI:
10.7554/eLife.23352.012
DOI:
10.7554/eLife.23352.013
DOI:
10.7554/eLife.23352.014
DOI:
10.7554/eLife.23352.015
DOI:
10.7554/eLife.23352.016
DOI:
10.7554/eLife.23352.017
DOI:
10.7554/eLife.23352.018
DOI:
10.7554/eLife.23352.019
DOI:
10.7554/eLife.23352.020
DOI:
10.7554/eLife.23352.021
DOI:
10.7554/eLife.23352.022
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
Permalink