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  • 1
    Online-Ressource
    Online-Ressource
    Association between MIC-1 and Type 2 Diabetes: A Combined Analysis
    Hindawi Limited ; 2019
    In:  Disease Markers Vol. 2019 ( 2019-11-16), p. 1-8
    Details
    In: Disease Markers, Hindawi Limited, Vol. 2019 ( 2019-11-16), p. 1-8
    Kurzfassung: Background and Objectives . Type 2 diabetes mellitus (T2DM) is an epidemic disease that endangers human health seriously. Recently, a large number of reports have revealed that macrophage-inhibiting cytokine-1 (MIC-1) is linked with T2DM, but the results were inconclusive. The aim of this study was to perform bioinformatics analysis of the association between MIC-1 and T2DM. Material and Methods . Datasets and relevant literatures were searched in Gene Expression Omnibus (GEO), PubMed, Google Scholar, and Web of Science till September 20, 2019. Expression levels of MIC-1 were extracted, pooled, and compared between T2DM cases and controls. Results . In summary, 11 GEO datasets and 3 articles with 421 T2DM cases and 711 controls were finally included. The expression level of MIC-1 was significantly higher in T2DM patients compared with controls, with a standard mean difference (SMD) of 0.54 and a 95% confidence interval (95% CI) of 0.24-0.83; in blood samples, the difference was still significant ( SMD = 0.65 ; 95 % CI = 0.24 ‐ 1.06 ). Meanwhile, the expression level of MIC-1 plays a significant role in differentiating T2DM cases from controls; the combined sensitivity, specificity, and odds ratio were 0.83 ( 95 % CI = 0.72 ‐ 0.90 ), 0.59 ( 95 % CI = 0.45 ‐ 0.72 ), and 1.64 ( 95 % CI = 1.35 ‐ 1.99 ), respectively. The summary receiver operating characteristic (SROC) curve demonstrated that the area under the curve (AUC) was 0.81 ( 95 % CI = 0.77 ‐ 0.84 ). Conclusion . Our results suggested that the expression levels of MIC-1 were significantly higher in T2DM patients in multiple tissues including blood samples.
    Materialart: Online-Ressource
    ISSN: 0278-0240 , 1875-8630
    URL: Article
    DOI: 10.1155/2019/7284691
    Sprache: Englisch
    Verlag: Hindawi Limited
    Publikationsdatum: 2019
    ZDB Id: 2033253-1
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    Sonic hedgehog promotes endothelial differentiation of bone marrow mesenchymal stem cells via VEGF-D
    AME Publishing Company ; 2018
    In:  Journal of Thoracic Disease Vol. 10, No. 9 ( 2018-09), p. 5476-5488
    Details
    In: Journal of Thoracic Disease, AME Publishing Company, Vol. 10, No. 9 ( 2018-09), p. 5476-5488
    Materialart: Online-Ressource
    ISSN: 2072-1439 , 2077-6624
    URL: Journal
    URL: Article
    DOI: 10.21037/jtd
    DOI: 10.21037/jtd.2018.09.50
    Sprache: Unbekannt
    Verlag: AME Publishing Company
    Publikationsdatum: 2018
    ZDB Id: 2573571-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Confinement effect of monolayer MoS 2 quantum dots on conjugated polyimide and promotion of solar-driven photocatalytic hydrogen generation
    Royal Society of Chemistry (RSC) ; 2017
    In:  Dalton Transactions Vol. 46, No. 12 ( 2017), p. 3877-3886
    Details
    In: Dalton Transactions, Royal Society of Chemistry (RSC), Vol. 46, No. 12 ( 2017), p. 3877-3886
    Materialart: Online-Ressource
    ISSN: 1477-9226 , 1477-9234
    URL: Article
    DOI: 10.1039/C6DT04916H
    Sprache: Englisch
    Verlag: Royal Society of Chemistry (RSC)
    Publikationsdatum: 2017
    ZDB Id: 1472887-4
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 4
    Online-Ressource
    Online-Ressource
    Visit-to-visit blood pressure variability is associated with gestational hypertension and pre-eclampsia
    Elsevier BV ; 2019
    In:  Pregnancy Hypertension Vol. 18 ( 2019-10), p. 126-131
    Details
    In: Pregnancy Hypertension, Elsevier BV, Vol. 18 ( 2019-10), p. 126-131
    Materialart: Online-Ressource
    ISSN: 2210-7789
    URL: Article
    DOI: 10.1016/j.preghy.2019.09.009
    Sprache: Englisch
    Verlag: Elsevier BV
    Publikationsdatum: 2019
    ZDB Id: 2584464-7
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 5
    Online-Ressource
    Online-Ressource
    Enhancement of Immunoregulatory Function of Modified Bone Marrow Mesenchymal Stem Cells by Targeting SOCS1
    Hindawi Limited ; 2018
    In:  BioMed Research International Vol. 2018 ( 2018), p. 1-10
    Details
    In: BioMed Research International, Hindawi Limited, Vol. 2018 ( 2018), p. 1-10
    Kurzfassung: Objective . The study aim to investigate the role of microRNA-155 (miR-155) on the immunoregulatory function of bone marrow mesenchymal stem cells (MSCs). Methods . MSCs were isolated from 2-week-old Sprague-Dawley rats and identified by flow cytometry using anti-CD29, anti-CD44, anti-CD34, and anti-CD45 antibodies. MSCs were transfected with miR155-mimics, miR155-inhibitor, and control oligos, respectively, and then cocultured with spleen mononuclear cells (SMCs). The mRNA levels of Th1, Th2, Th17, and Treg cell-specific transcription factors (Tbx21, Gata3, Rorc, and Foxp3, resp.) and the miR-155 target gene SOCS1 were detected by quantitative real-time PCR (qPCR) in SMCs. The proportion of CD4 + FOXP3 + Treg cells was detected by flow cytometry. In addition, the effects of MSCs transfected with miR-155 on the migration of rat SMCs were investigated by transwell chamber. Results . CD29 and CD44 were expressed in MSCs, while CD34 and CD45 were negative. The percentage of CD4 + FOXP3 + Treg cells in the SMC population was significantly higher compared with that noted in SMCs control group ( p 〈 0.001 ) following 72 hours of coculture with miR155-mimics-transfected SMCs. In contrast, the percentage of CD4 + FOXP3 + Treg cells in the SMCs cocultured with miR155-inhibitor-transfected MSCs was significantly lower compared with that noted in SMCs control group ( p 〈 0.001 ). MiR155-mimics-transfected MSCs inhibited the expression of Tbx21 , Rorc, and SOCS1 , while the expression of Gata3 and Foxp3 was increased. In contrast to the downregulation of the aforementioned genes, miR155-inhibitor-transfected MSCs resulted in upregulation of Tbx21 , Rorc , and SOCS1 expression levels and inhibition of Gata3 and Foxp3 . In the transwell assay, miR155-mimics-transfected MSCs exhibited lower levels of SMCs migration, while the miR155-inhibitor-transfected MSCs demonstrated significantly higher levels of migration, compared with the blank control group ( p 〈 0.01 , resp.). Conclusion . miR-155 favors the differentiation of T cells into Th2 and Treg cells in MSCs, while it inhibits the differentiation to Th1 and Th17 cells.
    Materialart: Online-Ressource
    ISSN: 2314-6133 , 2314-6141
    URL: Article
    DOI: 10.1155/2018/3530647
    Sprache: Englisch
    Verlag: Hindawi Limited
    Publikationsdatum: 2018
    ZDB Id: 2698540-8
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    Protective action of bone marrow mesenchymal stem cells in immune tolerance of allogeneic heart transplantation by regulating CD 45 RB + dendritic cells
    Wiley ; 2018
    In:  Clinical Transplantation Vol. 32, No. 4 ( 2018-04)
    Details
    In: Clinical Transplantation, Wiley, Vol. 32, No. 4 ( 2018-04)
    Kurzfassung: Bone marrow‐derived mesenchymal stem cells ( BMSC s) could exert a potent immunosuppressive effect and therefore may have a therapeutic potential in T‐cell‐dependent pathologies. We aimed to examine the effects of BMSC s on immune tolerance of allogeneic heart transplantation and the involvement of CD 45 RB + dendritic cells ( DC s). Methods Bone marrow‐derived DC s and BMSC s were co‐cultured, with CD 45 RB expression on the surface of DC s measured by flow cytometry. qRT ‐ PCR and Western blotting were used to detect mRNA and protein levels. Cytometric bead array was performed to determine the serum level of IL ‐10. Survival time of transplanted heart and expression of CD 4 + , CD 8 + , IL ‐2, IL ‐4, IL ‐10, IFN ‐γ were determined. Immunofluorescence assay was employed to determine intensity of C3d and C4d. Results DC s co‐cultured with BMSC s showed increased CD 45 RB and Foxp3 levels. CD 45 RB + DC s co‐cultured with T‐cells CD 4 + displayed increased T‐cell CD 4 + Foxp3 ratio and IL ‐10 than DC s. Both of them extended survival time of transplanted heart, decreased histopathological classification and score, intensity of C3d, C4d, proportion of CD 4 + , expression levels of IL ‐2 and IFN ‐γ, and increased the CD 4 + Foxp3 ratio and levels of IL ‐4 and IL ‐10. CD 45 RB + DC s achieved better protective effects than DC s. Conclusion BMSC s increased the expression of CD 45 RB in the bone marrow‐derived DC s, thereby strengthening immunosuppression capacity of T cells and immune tolerance of allogeneic heart transplantation.
    Materialart: Online-Ressource
    ISSN: 0902-0063 , 1399-0012
    URL: Issue
    URL: Article
    DOI: 10.1111/ctr.2018.32.issue-4
    DOI: 10.1111/ctr.13231
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2018
    ZDB Id: 2739458-X
    ZDB Id: 2004801-4
    Standort Signatur Einschränkungen Verfügbarkeit
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  • 7
    Online-Ressource
    Online-Ressource
    MicroRNA-132, Delivered by Mesenchymal Stem Cell-Derived Exosomes, Promote Angiogenesis in Myocardial Infarction
    Hindawi Limited ; 2018
    In:  Stem Cells International Vol. 2018 ( 2018-09-09), p. 1-11
    Details
    In: Stem Cells International, Hindawi Limited, Vol. 2018 ( 2018-09-09), p. 1-11
    Kurzfassung: Background . To cure ischemic diseases, angiogenesis needs to be improved by various strategies in ischemic area. Considering that microRNA-132 (miR-132) regulates endothelial cell behavior during angiogenesis and the safe and efficacious delivery of microRNAs in vivo is rarely achieved, an ideal vehicle for miR-132 delivery could bring the promise for ischemic diseases. As a natural carrier of biological molecules, exosomes are more and more developed as an ideal vehicle for miRNA transfer. Meanwhile, mesenchymal stem cells could release large amounts of exosomes. Thus, this study aimed to investigate whether MSC-derived exosomes can be used for miR-132 delivery in the treatment of myocardial ischemia. Methods . MSC-derived exosomes were electroporated with miR-132 mimics and inhibitors. After electroporation, miR-132 exosomes were labelled with DiI and added to HUVECs. Internalization of DiI-labelled exosomes was examined by fluorescent microscopy. Expression levels of miR-132 in exosomes and HUVECs were quantified by real-time PCR. The mRNA levels of miR-132 target gene RASA1 in HUVECs were quantified by real-time PCR. Luciferase reporter assay was performed to examine the targeting relationship between miR-132 and RASA1. The effects of miR-132 exosomes on the angiogenic ability of endothelial cells were evaluated by tube formation assay. Matrigel plug assay and myocardial infarction model were used to determine whether miR-132 exosomes can promote angiogenesis in vivo . Results . miR-132 mimics were effectively electroporated and highly detected in MSC-derived exosomes. The expression level of miR-132 was high in HUVECs preincubated with miR-132 mimic-electroporated exosomes and low in HUVECs preincubated with miR-132 inhibitor-electroporated exosomes. The expression level of RASA1, miR-132 target gene, was reversely correlated with miR-132 expression in HUVECs pretreated with exosomes. Luciferase reporter assay further confirmed that RASA1 was a direct target of miR-132. Exosomes loaded with miR-132, as a vehicle for miRNA transfer, significantly increased tube formation of endothelial cells. Moreover, subcutaneous injection of HUVECs pretreated with miR-132 exosomes in nude mice significantly increased their angiogenesis capacity in vivo . In addition, transplantation of miR-132 exosomes in the ischemic hearts of mice markedly enhanced the neovascularization in the peri-infarct zone and preserved heart functions. Conclusions . The findings suggest that the export of miR-132 via MSC-derived exosomes represents a novel strategy to enhance angiogenesis in ischemic diseases.
    Materialart: Online-Ressource
    ISSN: 1687-966X , 1687-9678
    URL: Article
    DOI: 10.1155/2018/3290372
    Sprache: Englisch
    Verlag: Hindawi Limited
    Publikationsdatum: 2018
    ZDB Id: 2573856-2
    Standort Signatur Einschränkungen Verfügbarkeit
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