GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Retracted: A transcriptomic study of native, expanded, and engineered human corneal endothelial cells
    Wiley ; 2019
    In:  Acta Ophthalmologica Vol. 97, No. S263 ( 2019-12)
    Details
    In: Acta Ophthalmologica, Wiley, Vol. 97, No. S263 ( 2019-12)
    Abstract: Human corneal endothelial cells (HCECs) fail to proliferate sufficiently following pathological injuries presumably due to their cell cycle arrest in the G1 phase (Joyce, 2012; Joyce et al, 1996). The current treatment for corneal endothelial disease includes partial (endothelial keratoplasty) or completes (penetrating keratoplasty) transplantation of the cornea. The shortage of available donor corneas worldwide continues to limit transplantation and thus necessitates alternative compensatory approaches. Stimulating of HCECs in vivo to promote cell proliferation or bioengineering of corneal endothelium for transplantation may offer practical solutions. Several methods for in vitro expansion and maintenance of HCECs have been developed until date but it is difficult to judge their reproducibility. Additionally, the cellular and molecular biology of these cells are not fully understood. Therefore, a comprehensive gene expression study on requisite cell‐cycle processes and associated signaling cascades may help to establish an important step for engineering of a functional corneal endothelium. Methods We attempted to develop a three step culture system to understand the gene expression profiles as well as the associated signaling pathways of native, proliferated and bioengineered HCECs corneal endothelium at different steps by RNA sequencing (RNA seq), quantitative real time PCR (qRT‐PCR) and scanning electron microscopy (SEM). Results Our results characterized 80 genes as markers of HCECs throughout the cell‐cycle stages, primarily focused on the associated cell cycle signaling cascades. Engaged genes were mostly related to entire cell‐cycle stages including the regulators of cell cycle and the cell cycle related signaling pathways suggesting their association with cell proliferation. Conclusion This study adds valuable information to the literature on biology of HCECs and forms a potential for prospective clinical applications of the data for advancing the field of corneal regenerative medicine.
    Type of Medium: Online Resource
    ISSN: 1755-375X , 1755-3768
    URL: Issue
    URL: Article
    DOI: 10.1111/aos.v97.s263
    DOI: 10.1111/j.1755-3768.2019.5242
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2466981-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Substrates for Expansion of Corneal Endothelial Cells towards Bioengineering of Human Corneal Endothelium
    MDPI AG ; 2015
    In:  Journal of Functional Biomaterials Vol. 6, No. 3 ( 2015-09-11), p. 917-945
    Details
    In: Journal of Functional Biomaterials, MDPI AG, Vol. 6, No. 3 ( 2015-09-11), p. 917-945
    Type of Medium: Online Resource
    ISSN: 2079-4983
    URL: Article
    DOI: 10.3390/jfb6030917
    Language: English
    Publisher: MDPI AG
    Publication Date: 2015
    detail.hit.zdb_id: 2648525-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Tear Metabolomics in Dry Eye Disease: A Review
    MDPI AG ; 2019
    In:  International Journal of Molecular Sciences Vol. 20, No. 15 ( 2019-08-01), p. 3755-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 20, No. 15 ( 2019-08-01), p. 3755-
    Abstract: Dry eye disease (DED) is a multifactorial syndrome that can be caused by alteration in the quality or quantity of the precorneal tear film. It is considered one of the most common ocular conditions leading patients to seek eye care. The current method for diagnostic evaluations and follow-up examinations of DED is a combination of clinical signs and symptoms determined by clinical tests and questionnaires, respectively. The application of powerful omics technologies has opened new avenues toward analysis of subjects in health and disease. Metabolomics is a new emerging and complementary research discipline to all modern omics in the comprehensive analysis of biological systems. The identification of distinct metabolites and integrated metabolic profiles in patients can potentially inform clinicians at an early stage or during monitoring of disease progression, enhancing diagnosis, prognosis, and the choice of therapy. In ophthalmology, metabolomics has gained considerable attention over the past decade but very limited such studies have been reported on DED. This paper aims to review the application of tear metabolomics in DED.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms20153755
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2019364-6
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Hyaluronan-Based Hydrogel Scaffolds for Limbal Stem Cell Transplantation: A Review
    MDPI AG ; 2019
    In:  Cells Vol. 8, No. 3 ( 2019-03-14), p. 245-
    Details
    In: Cells, MDPI AG, Vol. 8, No. 3 ( 2019-03-14), p. 245-
    Abstract: Hyaluronan (HA), also termed hyaluronic acid or hyaluronate, is a major component of the extracellular matrix. This non-sulfated glycosaminoglycan plays a key role in cell proliferation, growth, survival, polarization, and differentiation. The diverse biological roles of HA are linked to the combination of HA’s physicochemical properties and HA-binding proteins. These unique characteristics have encouraged the application of HA-based hydrogel scaffolds for stem cell-based therapy, a successful method in the treatment of limbal stem cell deficiency (LSCD). This condition occurs following direct damage to limbal stem cells and/or changes in the limbal stem cell niche microenvironment due to intrinsic and extrinsic insults. This paper reviews the physical properties, synthesis, and degradation of HA. In addition, the interaction of HA with other extracellular matrix (ECM) components and receptor proteins are discussed. Finally, studies employing HA-based hydrogel scaffolds in the treatment of LSCD are reviewed.
    Type of Medium: Online Resource
    ISSN: 2073-4409
    URL: Article
    DOI: 10.3390/cells8030245
    Language: English
    Publisher: MDPI AG
    Publication Date: 2019
    detail.hit.zdb_id: 2661518-6
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...